CatLogenzyme kinetics catalog

EC 1.1.1.158: UDP-N-acetylmuramate dehydrogenase

OxidoreductasesOpen in Browse

CatLog read 31 records for EC 1.1.1.158 from 1 paper, for 1 organism. Names in these papers: UDP-N-acetylenolpyruvylglucosamine reductase (MurB).

EC 1.1.1.158 is no longer current; BRENDA: “transferred, now EC 1.3.1.98”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

PaperOrganismsRecords
Steady-state kinetic mechanism of Escherichia coli UDP-N-acetylenolpyruvylglucosamine reductaseEscherichia coli31

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia colialpha-nicotinamide adenine dinucleotide phosphate reduced form420.7854 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia colibeta-nicotinamide adenine dinucleotide 3'-phosphate reduced form490.25200 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane pH 8.0; UNAGEP fixed at 125 µM360.0221.6 × 10³ calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane pH 8.0; UNAGEP fixed at 125 µM140.043330 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane pH 8.0; UNAGEP fixed at 125 µM160.045360 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane pH 8.0; UNAGEP fixed at 125 µM120.028430 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane pH 8.0; UNAGEP fixed at 125 µM260.0122.2 × 10³ calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane pH 8.0; UNAGEP fixed at 125 µM4.80.021230 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane pH 8.0; UNAGEP fixed at 125 µM40.06265 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane pH 8.0; UNAGEP fixed at 2500 µM10.175.9 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH320.1320 calc.pH 6, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPHUNAGEP fixed at 15 µM (subsaturating)350.0172.1 × 10³ calc.pH 7, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane adjusted to pH with HCl; apparent Km400.0113.6 × 10³ calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH8 x 8 matrix of UNAGEP and NADPH each at 7; 16; 22; 37; 73—0.017—pH 7, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH50 mM Bis-Tris propane adjusted to pH with HCl; 20; 30; 50—0.017—pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliNADPH—0.02—pH 9, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia colinicotinamide hypoxanthine dinucleotide phosphate reduced form360.047770 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia colithionicotinamide adenine dinucleotide phosphate—0.27—pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane pH 8.0; NADPH fixed at 150 µM570.022.8 × 10³ calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane pH 8.0; NADPH fixed at 150 µM160.046350 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane pH 8.0; NADPH fixed at 150 µM350.0132.7 × 10³ calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane pH 8.0; NADPH fixed at 150 µM100.027370 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane pH 8.0; NADPH fixed at 150 µM260.0171.5 × 10³ calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane pH 8.0; NADPH fixed at 150 µM4.90.034140 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane pH 8.0; NADPH fixed at 150 µM4.80.042110 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane pH 8.0; NADPH fixed at 1000 µM2.60.357.4 calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine400.0251.6 × 10³ calc.pH 6, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamineNADPH fixed at 150 µM480.0182.7 × 10³ calc.pH 7, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine8 x 8 matrix of UNAGEP and NADPH each at 7; 16; 22; 37; 73510.0192.7 × 10³ calc.pH 7, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine50 mM Bis-Tris propane adjusted to pH with HCl; 20; 30; 50620.0242.6 × 10³ calc.pH 8, 37 °CPMID 7727397Open
UDP-N-acetylenolpyruvylglucosamine reductase (MurB)Escherichia coliUDP-N-acetylenolpyruvylglucosamine410.042980 calc.pH 9, 37 °CPMID 7727397Open