CatLogenzyme kinetics catalog

EC 1.14.13.225: F-actin monooxygenase

OxidoreductasesOpen in Browse

CatLog read 15 records for EC 1.14.13.225 from 1 paper, for 1 organism. Names in these papers: MICAL1 (full-length), MICAL1 MOCH (flavoprotein + CH domains), MICAL1 MOCHLIM (flavoprotein + CH + LIM domains).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 15 records for EC 1.14.13.225 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.13.225 in Browse.

Papers

PaperOrganismsRecords
Properties and catalytic activities of MICAL1, the flavoenzyme involved in cytoskeleton dynamics, and modulation by its CH, LIM and C-terminal domainsHomo sapiens15

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
MICAL1 (full-length)Homo sapiensF-actin (filamentous rabbit skeletal muscle actin, Cytoskeleton AKL99)7.80.029276pH 7.7, 25 °CPMID 26845023Open
MICAL1 (full-length)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)NADPH oxidase reaction (H2O2-producing)0.280.3750.75pH 7, 25 °CPMID 26845023Open
MICAL1 (full-length)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)NADPH oxidase reaction; whole data set fitted to Eq. (6)0.30.3980.73pH 7, 25 °CPMID 26845023Open
MICAL1 (full-length)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)no F-actin; NADPH varied 100–700 μM; enzyme 1250 nM0.84.6520.17pH 7.7, 25 °CPMID 26845023Open
MICAL1 (full-length)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)F-actin fixed at 4.3 μM; NADPH varied 10–400 μM1.90.02964.8pH 7.7, 25 °CPMID 26845023Open
MICAL1 MOCH (flavoprotein + CH domains)Homo sapiensF-actin (filamentous rabbit skeletal muscle actin, Cytoskeleton AKL99)260.00279487pH 7.7, 25 °CPMID 26845023Open
MICAL1 MOCH (flavoprotein + CH domains)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)NADPH oxidase reaction (H2O2-producing)2.50.13418.5pH 7, 25 °CPMID 26845023Open
MICAL1 MOCH (flavoprotein + CH domains)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)NADPH oxidase reaction; whole data set fitted to Eq. (6)2.60.11123.2pH 7, 25 °CPMID 26845023Open
MICAL1 MOCH (flavoprotein + CH domains)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)no F-actin; NADPH varied 10–500 μM; enzyme 190 nM1.70.4593.8pH 7.7, 25 °CPMID 26845023Open
MICAL1 MOCH (flavoprotein + CH domains)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)F-actin fixed at 4.2 μM; NADPH varied 10–200 μM; enzyme 3 nM210.05423pH 7.7, 25 °CPMID 26845023Open
MICAL1 MOCHLIM (flavoprotein + CH + LIM domains)Homo sapiensF-actin (filamentous rabbit skeletal muscle actin, Cytoskeleton AKL99)150.00443.39 × 10³pH 7.7, 25 °CPMID 26845023Open
MICAL1 MOCHLIM (flavoprotein + CH + LIM domains)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)NADPH oxidase reaction (H2O2-producing)3.50.2315.3pH 7, 25 °CPMID 26845023Open
MICAL1 MOCHLIM (flavoprotein + CH + LIM domains)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)NADPH oxidase reaction; whole data set fitted to Eq. (6)2.90.17816.5pH 7, 25 °CPMID 26845023Open
MICAL1 MOCHLIM (flavoprotein + CH + LIM domains)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)no F-actin; NADPH varied 25–1000 μM; enzyme 125 nM4.30.9664.5pH 7.7, 25 °CPMID 26845023Open
MICAL1 MOCHLIM (flavoprotein + CH + LIM domains)Homo sapiensnicotinamide adenine dinucleotide phosphate, reduced form (NADPH)F-actin fixed at 4.4 μM; NADPH varied 20–200 μM6.40.014448pH 7.7, 25 °CPMID 26845023Open