EC 1.14.15.30: 3-ketosteroid 9alpha-monooxygenase
OxidoreductasesOpen in Browse
CatLog read 24 records for EC 1.14.15.30 from 3 papers, for 2 organisms. Names in these papers: KshA5, KshA1, 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase), 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 15 records for EC 1.14.15.30 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.15.30 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Substrate specificities and conformational flexibility of 3-ketosteroid 9α-hydroxylases | Rhodococcus rhodochrous | 15 |
| Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis | Mycobacterium tuberculosis | 5 |
| Characterization of 3-ketosteroid 9α-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis | Mycobacterium tuberculosis | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB) | Mycobacterium tuberculosis | 1,4-androstadiene-3,17-dione | 0.8 | 0.11 | 7.6 | pH 7, 25 °C | PMID 19234303 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB), 1,4-androstadiene-3,17-dione |
| 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB) | Mycobacterium tuberculosis | 4-androstene-3,17-dione | 0.07 | 0.024 | 3 | pH 7, 25 °C | PMID 19234303 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB), 4-androstene-3,17-dione |
| 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB) | Mycobacterium tuberculosis | dioxygenapparent kcat/Km | — | — | 2.45 | pH 7, 25 °C | PMID 19234303 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB), dioxygen |
| 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB) | Mycobacterium tuberculosis | dioxygenapparent kcat; apparent Km | 110 | 46 | 2.4 calc. | pH 7, 25 °C | PMID 19234303 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB: oxygenase KshA reconstituted with reductase KshB), dioxygen |
| 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase) | Mycobacterium tuberculosis | 3-oxo-23,24-bisnorchol-4-en-22-oic acid | 0.08 | 0.003 | 30 | pH 7, 22 °C | PMID 21987574 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase), 3-oxo-23,24-bisnorchol-4-en-22-oic acid |
| 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase) | Mycobacterium tuberculosis | 3-oxo-23,24-bisnorchol-4-en-22-oyl-coenzyme A thioester | 0.61 | 0.0068 | 90 | pH 7, 22 °C | PMID 21987574 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase), 3-oxo-23,24-bisnorchol-4-en-22-oyl-coenzyme A thioester |
| 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase) | Mycobacterium tuberculosis | 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid | 0.25 | 0.07 | 3.5 | pH 7, 22 °C | PMID 21987574 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase), 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid |
| 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase) | Mycobacterium tuberculosis | 3-oxo-23,24-bisnorchola-1,4-dien-22-oyl-coenzyme A thioester | 2.7 | 0.017 | 160 | pH 7, 22 °C | PMID 21987574 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase), 3-oxo-23,24-bisnorchola-1,4-dien-22-oyl-coenzyme A thioester |
| 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase) | Mycobacterium tuberculosis | dioxygen | 2.5 | 0.09 | 29 | pH 7, 22 °C | PMID 21987574 | Open record: 3-ketosteroid 9α-hydroxylase (KshAB; KshA oxygenase + KshB reductase), dioxygen |
| KshA1 | Rhodococcus rhodochrous | 1,4-androstadiene-3,17-dione | 1.3 | 0.05 | 27 | pH 7, 22 °C | PMID 25049233 | Open record: KshA1, 1,4-androstadiene-3,17-dione |
| KshA1 | Rhodococcus rhodochrous | 3-oxo-23,24-bisnorchol-4-en-22-oic acid | 2.6 | 0.0022 | 1.2 × 10³ | pH 7, 22 °C | PMID 25049233 | Open record: KshA1, 3-oxo-23,24-bisnorchol-4-en-22-oic acid |
| KshA1 | Rhodococcus rhodochrous | 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oate | 0.9 | 0.001 | 900 | pH 7, 22 °C | PMID 25049233 | Open record: KshA1, 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oate |
| KshA1 | Rhodococcus rhodochrous | 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oyl CoA | 1.1 | 0.002 | 600 | pH 7, 22 °C | PMID 25049233 | Open record: KshA1, 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oyl CoA |
| KshA1 | Rhodococcus rhodochrous | 4-Estren-3,17-dione | 0.5 | 0.4 | 1.2 | pH 7, 22 °C | PMID 25049233 | Open record: KshA1, 4-Estren-3,17-dione |
| KshA1 | Rhodococcus rhodochrous | O2 | 1.7 | 0.0015 | 1.1 × 10³ | pH 7, 22 °C | PMID 25049233 | Open record: KshA1, O2 |
| KshA1 | Rhodococcus rhodochrous | Testosterone | 1.4 | 0.11 | 13 | pH 7, 22 °C | PMID 25049233 | Open record: KshA1, Testosterone |
| KshA5 | Rhodococcus rhodochrous | 1,4-androstadiene-3,17-dione | 0.7 | — | — | pH 7, 22 °C | PMID 25049233 | Open record: KshA5, 1,4-androstadiene-3,17-dione |
| KshA5 | Rhodococcus rhodochrous | 3-oxo-23,24-bisnorchol-4-en-22-oic acid | 0.6 | 0.0012 | 500 | pH 7, 22 °C | PMID 25049233 | Open record: KshA5, 3-oxo-23,24-bisnorchol-4-en-22-oic acid |
| KshA5 | Rhodococcus rhodochrous | 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oate | 0.5 | 5 × 10⁻⁴ | 1 × 10³ | pH 7, 22 °C | PMID 25049233 | Open record: KshA5, 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oate |
| KshA5 | Rhodococcus rhodochrous | 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oyl CoA | 1.7 | 0.5 | 3.7 | pH 7, 22 °C | PMID 25049233 | Open record: KshA5, 3-oxo-23,24-bisnorcholesta-1,4-diene-22-oyl CoA |
| KshA5 | Rhodococcus rhodochrous | 4-Estren-3,17-dione | 0.8 | 8 × 10⁻⁴ | 1 × 10³ | pH 7, 22 °C | PMID 25049233 | Open record: KshA5, 4-Estren-3,17-dione |
| KshA5 | Rhodococcus rhodochrous | 5α-Androstan-3,17-dione | 0.8 | 0.0061 | 140 | pH 7, 22 °C | PMID 25049233 | Open record: KshA5, 5α-Androstan-3,17-dione |
| KshA5 | Rhodococcus rhodochrous | O2 | 0.8 | 0.049 | 17 | pH 7, 22 °C | PMID 25049233 | Open record: KshA5, O2 |
| KshA5 | Rhodococcus rhodochrous | Testosterone | 0.8 | 5 × 10⁻⁴ | 1.5 × 10³ | pH 7, 22 °C | PMID 25049233 | Open record: KshA5, Testosterone |