CatLogenzyme kinetics catalog

EC 1.3.99.5: 3-oxo-5alpha-steroid 4-dehydrogenase (acceptor)

OxidoreductasesOpen in Browse

CatLog read 9 records for EC 1.3.99.5 from 2 papers, for 3 organisms. Names in these papers: steroid 5alpha-reductase, 3-ketosteroid Δ4-(5α)-dehydrogenase (Δ4-(5α)-KSTD, Ro05698).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 7 records for EC 1.3.99.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.3.99.5 in Browse.

Papers

PaperOrganismsRecords
Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductasesHomo sapiens, Rattus norvegicus6
Structure and catalytic mechanism of 3-ketosteroid-Delta4-(5α)-dehydrogenase from Rhodococcus jostii RHA1 genomeRhodococcus jostii3

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
3-ketosteroid Δ4-(5α)-dehydrogenase (Δ4-(5α)-KSTD, Ro05698)Rhodococcus jostii1-(5α)-androstene-3,17-dione2.40.012.4 × 10³pH 7.4, 30 °CPMID 22833669Open
3-ketosteroid Δ4-(5α)-dehydrogenase (Δ4-(5α)-KSTD, Ro05698)variant S468TRhodococcus jostii1-(5α)-androstene-3,17-dione1.60.179pH 7.4, 30 °CPMID 22833669Open
3-ketosteroid Δ4-(5α)-dehydrogenase (Δ4-(5α)-KSTD, Ro05698)variant W136FRhodococcus jostii1-(5α)-androstene-3,17-dione0.70.06610pH 7.4, 30 °CPMID 22833669Open
steroid 5alpha-reductaseHomo sapiensandrostenedione—0.0017—pH 7, 37 °CPMID 2339109Open
steroid 5alpha-reductaseHomo sapiensprogesterone—8 × 10⁻⁴—pH 7, 37 °CPMID 2339109Open
steroid 5alpha-reductaseHomo sapienstestosterone—0.0036—pH 7, 37 °CPMID 2339109Open
steroid 5alpha-reductaseRattus norvegicusandrostenedione—0.0028—pH 6.6, 37 °CPMID 2339109Open
steroid 5alpha-reductaseRattus norvegicusprogesterone—5 × 10⁻⁴—pH 6.6, 37 °CPMID 2339109Open
steroid 5alpha-reductaseRattus norvegicustestosterone—0.0025—pH 6.6, 37 °CPMID 2339109Open