CatLogenzyme kinetics catalog

EC 1.5.99.12: cytokinin dehydrogenase

OxidoreductasesOpen in Browse

CatLog read 56 records for EC 1.5.99.12 from 2 papers, for 1 organism. Names in these papers: cytokinin dehydrogenase ZmCKX1, cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX), cytokinin dehydrogenase (ZmCKX1).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 32 records for EC 1.5.99.12 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.5.99.12 in Browse.

Papers

PaperOrganismsRecords
Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptorsZea mays48
High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolyticaZea mays8

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
cytokinin dehydrogenase (ZmCKX1)Zea maysN6-(2-isopentenyl)adenineRosmaric0.4——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase (ZmCKX1)Zea maysN6-(2-isopentenyl)adeninecaffeic0.3——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase (ZmCKX1)Zea maysN6-(2-isopentenyl)adenineo-coumaric0.3——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase (ZmCKX1)Zea maysN6-(2-isopentenyl)adeninehydrocaffeic0.2——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays1,4-naphthoquinone4-aminophenol assay of 3-methyl-2-butenal formation169.6——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays1,4-naphthoquinone4-aminophenol aldehyde assay—0.011—pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2,3-dimethoxy-5-methyl-1,4-benzoquinone4-aminophenol assay of 3-methyl-2-butenal formation143.6——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2,3-dimethoxy-5-methyl-1,4-benzoquinone4-aminophenol aldehyde assay—0.21—pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone123.1——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2,6-dichlorophenol indophenol4-aminophenol assay of 3-methyl-2-butenal formation178.4——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2,6-dichlorophenol indophenol278——pH 8, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2,6-dichlorophenol indophenol4-aminophenol aldehyde assay—0.036—pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2-methyl-1,4-naphthoquinone85.4——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2-methyl-3-phytyl-1,4-naphthoquinone2.3——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mays2-methyl-3-phytyl-1,4-naphthoquinone1.6——pH 8, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysDaphnoretin1——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysDaphnoretin4.2——pH 8, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea mayshorse heart cytochrome c0.8——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-furfuryl)adenine0.15 mM substrate; 0.5 mM Q0 as electron acceptor1.2——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-furfuryl)adenine0.15 mM substrate; aerobic; cosubstrate O20.7——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-furfuryl)adenineaerobic; no electron acceptor added (O2 as acceptor)—0.07—pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-hydroxybenzyl)adenine0.15 mM substrate; 0.5 mM Q0 as electron acceptor0.1——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-hydroxybenzyl)adenine0.15 mM substrate; aerobic; cosubstrate O20.1——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-hydroxybenzyl)adenineaerobic; no electron acceptor added (O2 as acceptor)—0.04—pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-isopentenyl)adenine0.15 mM substrate; 0.5 mM Q0 as electron acceptor143.6——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-isopentenyl)adenine0.15 mM substrate; aerobic; cosubstrate O20.6——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-isopentenyl)adenine0.5 mM Q0 as electron acceptor—0.04—pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-isopentenyl)adenineaerobic; no electron acceptor added (O2 as acceptor)—0.44—pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-isopentenyl)adenosine0.15 mM substrate; 0.5 mM Q0 as electron acceptor129.8——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-isopentenyl)adenosine0.15 mM substrate; aerobic; cosubstrate O20.7——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-isopentenyl)adenosine0.5 mM Q0 as electron acceptor—0.044—pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-isopentenyl)adenosine-5'-monophosphate0.5——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-methoxybenzyl)adenine0.5 mM Q0 as electron acceptor0.7——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(2-methoxybenzyl)adenineaerobic; no electron acceptor added (O2 as acceptor)0.4——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(3-hydroxybenzyl)adenine0.1——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(3-methoxybenzyl)adenine0.2——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(4-hydroxybenzyl)adenine0.5 mM Q0 as electron acceptor0.8——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(4-hydroxybenzyl)adenineaerobic; no electron acceptor added (O2 as acceptor)0.8——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(4-methoxybenzyl)adenine0.5 mM Q0 as electron acceptor1——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-(4-methoxybenzyl)adenineaerobic; no electron acceptor added (O2 as acceptor)0.5——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-benzyladenine0.2——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-benzyladenosine-5'-monophosphate0.1——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-methyl-N6-(3-methyl-2-butenyl)adenine0.5 mM Q0 as electron acceptor0.6——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysN6-methyl-N6-(3-methyl-2-butenyl)adenineaerobic; no electron acceptor added (O2 as acceptor)0.4——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysO20.6——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysplantacyanin0.9——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysScopoletin1.4——pH 6.5, 37 °CPMID 14965342Open
cytokinin dehydrogenase ZmCKX1Zea maysScopoletin1.1——pH 8, 37 °CPMID 14965342Open
cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX)Zea maysN6-(Δ2-isopentenyl)adenineoxidase mode (O2 as electron acceptor, no DCPIP)0.155 × 10⁻⁴300pH 7.4, 30 °CPMID 15927342Open
cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX)Zea maysN6-(Δ2-isopentenyl)adeninecosubstrate DCPIP1280.00111.16 × 10⁵pH 7.4, 30 °CPMID 15927342Open
cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX)Zea maysN6-(Δ2-isopentenyl)adenosineoxidase mode (O2 as electron acceptor, no DCPIP)0.220.0019120pH 7.4, 30 °CPMID 15927342Open
cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX)Zea maysN6-(Δ2-isopentenyl)adenosinecosubstrate DCPIP620.0125.2 × 10³pH 7.4, 30 °CPMID 15927342Open
cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX)Zea maysZeatinoxidase mode (O2 as electron acceptor, no DCPIP)0.270.0015180pH 7.4, 30 °CPMID 15927342Open
cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX)Zea maysZeatincosubstrate DCPIP1350.0091.5 × 10⁴pH 7.4, 30 °CPMID 15927342Open
cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX)Zea maysZeatin ribosideoxidase mode (O2 as electron acceptor, no DCPIP)0.160.00280pH 7.4, 30 °CPMID 15927342Open
cytokinin oxidase/dehydrogenase ZmCKO1 (CKO/CKX)Zea maysZeatin ribosidecosubstrate DCPIP210.063300pH 7.4, 30 °CPMID 15927342Open