EC 1.7.1.17: FMN-dependent NADH-azoreductase
OxidoreductasesOpen in Browse
CatLog read 63 records for EC 1.7.1.17 from 5 papers, for 3 organisms. Names in these papers: azoreductase AzoA, azoreductase (soAzoR), azoreductase 1 (paAzoR1), azoreductase (AzoA) and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 25 records for EC 1.7.1.17 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.7.1.17 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Probing the NADH- and Methyl Red-binding site of a FMN-dependent azoreductase (AzoA) from Enterococcus faecalis | Enterococcus faecalis | 21 |
| Expansion of the active site of the azoreductase from Shewanella oneidensis MR-1 | Shewanella oneidensis MR-1 | 18 |
| Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide | Pseudomonas aeruginosa | 12 |
| Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysis | Enterococcus faecalis | 10 |
| Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis | Enterococcus faecalis | 2 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| aerobic FMN-dependent azoreductase AzoA | Enterococcus faecalis | 2-[4-(dimethylamino)phenylazo]benzoic acid | — | 0.024 | — | pH 7.1, 23 °C | PMID 15003265 | Open record: aerobic FMN-dependent azoreductase AzoA, 2-[4-(dimethylamino)phenylazo]benzoic acid |
| aerobic FMN-dependent azoreductase AzoA | Enterococcus faecalis | NADH | — | 0.14 | — | pH 7.1, 23 °C | PMID 15003265 | Open record: aerobic FMN-dependent azoreductase AzoA, NADH |
| azoreductase (AzoA) | Enterococcus faecalis | methyl red | — | 0.0254 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA), methyl red |
| azoreductase (AzoA) | Enterococcus faecalis | NADH | — | 0.158 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA), NADH |
| azoreductase (AzoA)variant W105F | Enterococcus faecalis | methyl red | — | 0.0247 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA) variant W105F, methyl red |
| azoreductase (AzoA)variant W105F | Enterococcus faecalis | NADH | — | 0.175 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA) variant W105F, NADH |
| azoreductase (AzoA)variant W105H | Enterococcus faecalis | methyl red | — | 0.0251 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA) variant W105H, methyl red |
| azoreductase (AzoA)variant W105H | Enterococcus faecalis | NADH | — | 0.171 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA) variant W105H, NADH |
| azoreductase (AzoA)variant W105Q | Enterococcus faecalis | methyl red | — | 0.0282 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA) variant W105Q, methyl red |
| azoreductase (AzoA)variant W105Q | Enterococcus faecalis | NADH | — | 0.169 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA) variant W105Q, NADH |
| azoreductase (AzoA)variant W105Y | Enterococcus faecalis | methyl red | — | 0.0276 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA) variant W105Y, methyl red |
| azoreductase (AzoA)variant W105Y | Enterococcus faecalis | NADH | — | 0.166 | — | pH 7.1, 23 °C | PMID 18757799 | Open record: azoreductase (AzoA) variant W105Y, NADH |
| azoreductase (soAzoR) | Shewanella oneidensis MR-1 | methyl red | 33.86 | 0.01155 | 2.93 × 10³ | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR), methyl red |
| azoreductase (soAzoR) | Shewanella oneidensis MR-1 | NADH | 33.9 | 0.1878 | 180 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR), NADH |
| azoreductase (soAzoR)variant F117A | Shewanella oneidensis MR-1 | methyl red | 70.36 | 0.01603 | 4.39 × 10³ | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant F117A, methyl red |
| azoreductase (soAzoR)variant F117A | Shewanella oneidensis MR-1 | NADH | 68.23 | 0.02714 | 2.51 × 10³ | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant F117A, NADH |
| azoreductase (soAzoR)variant G140F | Shewanella oneidensis MR-1 | NADH | 3.49 | 0.2897 | 10 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant G140F, NADH |
| azoreductase (soAzoR)variant G141F | Shewanella oneidensis MR-1 | NADH | 4.82 | 0.3671 | 10 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant G141F, NADH |
| azoreductase (soAzoR)variant H143A | Shewanella oneidensis MR-1 | methyl red | 6.35 | 0.06872 | 90 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant H143A, methyl red |
| azoreductase (soAzoR)variant H143A | Shewanella oneidensis MR-1 | NADH | 29.39 | 0.4338 | 70 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant H143A, NADH |
| azoreductase (soAzoR)variant N96A | Shewanella oneidensis MR-1 | methyl red | 2.9 | 0.09315 | 30 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant N96A, methyl red |
| azoreductase (soAzoR)variant N96A | Shewanella oneidensis MR-1 | NADH | 14.42 | 0.3158 | 50 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant N96A, NADH |
| azoreductase (soAzoR)variant R139A | Shewanella oneidensis MR-1 | methyl red | 21.48 | 0.04809 | 450 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant R139A, methyl red |
| azoreductase (soAzoR)variant R139A | Shewanella oneidensis MR-1 | NADH | 30.2 | 0.2241 | 140 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant R139A, NADH |
| azoreductase (soAzoR)variant R59A | Shewanella oneidensis MR-1 | methyl red | 23.68 | 0.0296 | 800 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant R59A, methyl red |
| azoreductase (soAzoR)variant R59A | Shewanella oneidensis MR-1 | NADH | 32.13 | 0.3433 | 90 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant R59A, NADH |
| azoreductase (soAzoR)variant S16A | Shewanella oneidensis MR-1 | methyl red | 9.8 | 0.07676 | 130 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant S16A, methyl red |
| azoreductase (soAzoR)variant S16A | Shewanella oneidensis MR-1 | NADH | 19.18 | 0.5307 | 40 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant S16A, NADH |
| azoreductase (soAzoR)variant Y119A | Shewanella oneidensis MR-1 | methyl red | 20.02 | 0.01928 | 1.04 × 10³ | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant Y119A, methyl red |
| azoreductase (soAzoR)variant Y119A | Shewanella oneidensis MR-1 | NADH | 17.17 | 0.6551 | 30 | pH 7.4, 30 °C | PMID 29100166 | Open record: azoreductase (soAzoR) variant Y119A, NADH |
| azoreductase 1 (paAzoR1) | Pseudomonas aeruginosa | balsalazideapparent kcat; apparent Km | 36.8 | 0.0986 | 373 calc. | PMID 20057057 | Open record: azoreductase 1 (paAzoR1), balsalazide | |
| azoreductase 1 (paAzoR1) | Pseudomonas aeruginosa | balsalazideapparent kcat/Km | — | — | 373 | PMID 20057057 | Open record: azoreductase 1 (paAzoR1), balsalazide | |
| azoreductase 1 (paAzoR1) | Pseudomonas aeruginosa | methyl redapparent kcat; apparent Km | 12.7 | 0.0927 | 137 calc. | PMID 20057057 | Open record: azoreductase 1 (paAzoR1), methyl red | |
| azoreductase 1 (paAzoR1) | Pseudomonas aeruginosa | methyl redapparent kcat/Km | — | — | 137 | PMID 20057057 | Open record: azoreductase 1 (paAzoR1), methyl red | |
| azoreductase 1 (paAzoR1) | Pseudomonas aeruginosa | reduced nicotinamide adenine dinucleotide | 10 | 0.538 | 19 calc. | PMID 20057057 | Open record: azoreductase 1 (paAzoR1), reduced nicotinamide adenine dinucleotide | |
| azoreductase 1 (paAzoR1) | Pseudomonas aeruginosa | reduced nicotinamide adenine dinucleotide phosphate | 33.6 | 1.197 | 28.1 calc. | PMID 20057057 | Open record: azoreductase 1 (paAzoR1), reduced nicotinamide adenine dinucleotide phosphate | |
| azoreductase 1 (paAzoR1)variant Y131F | Pseudomonas aeruginosa | balsalazideapparent kcat; apparent Km | 24.1 | 0.1029 | 234 calc. | PMID 20057057 | Open record: azoreductase 1 (paAzoR1) variant Y131F, balsalazide | |
| azoreductase 1 (paAzoR1)variant Y131F | Pseudomonas aeruginosa | balsalazideapparent kcat/Km | — | — | 234 | PMID 20057057 | Open record: azoreductase 1 (paAzoR1) variant Y131F, balsalazide | |
| azoreductase 1 (paAzoR1)variant Y131F | Pseudomonas aeruginosa | methyl redapparent kcat; apparent Km | 19.1 | 0.0445 | 429 calc. | PMID 20057057 | Open record: azoreductase 1 (paAzoR1) variant Y131F, methyl red | |
| azoreductase 1 (paAzoR1)variant Y131F | Pseudomonas aeruginosa | methyl redapparent kcat/Km | — | — | 429 | PMID 20057057 | Open record: azoreductase 1 (paAzoR1) variant Y131F, methyl red | |
| azoreductase 1 (paAzoR1)variant Y131F | Pseudomonas aeruginosa | reduced nicotinamide adenine dinucleotide | 25.2 | 0.965 | 26.1 calc. | PMID 20057057 | Open record: azoreductase 1 (paAzoR1) variant Y131F, reduced nicotinamide adenine dinucleotide | |
| azoreductase 1 (paAzoR1)variant Y131F | Pseudomonas aeruginosa | reduced nicotinamide adenine dinucleotide phosphate | 35.5 | 0.535 | 66.4 calc. | PMID 20057057 | Open record: azoreductase 1 (paAzoR1) variant Y131F, reduced nicotinamide adenine dinucleotide phosphate | |
| azoreductase AzoA | Enterococcus faecalis | Methyl Red | 59.1 | 0.024 | 2.46 × 10³ | pH 7 | PMID 22387379 | Open record: azoreductase AzoA, Methyl Red |
| azoreductase AzoA | Enterococcus faecalis | NADH | — | 0.143 | 410 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA, NADH |
| azoreductase AzoAvariant A123F | Enterococcus faecalis | Methyl Red | 245 | 0.0485 | 5.05 × 10³ | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant A123F, Methyl Red |
| azoreductase AzoAvariant A123F | Enterococcus faecalis | NADH | — | 0.411 | 600 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant A123F, NADH |
| azoreductase AzoAvariant E16G | Enterococcus faecalis | NADH | 20.8 | 0.25 | 83 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant E16G, NADH |
| azoreductase AzoAvariant L59G | Enterococcus faecalis | Methyl Red | 6.61 | 0.0288 | 230 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant L59G, Methyl Red |
| azoreductase AzoAvariant L59G | Enterococcus faecalis | NADH | — | 2.298 | 2.9 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant L59G, NADH |
| azoreductase AzoAvariant N106A | Enterococcus faecalis | Methyl Red | 1.2 | 0.0112 | 110 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant N106A, Methyl Red |
| azoreductase AzoAvariant N106A | Enterococcus faecalis | NADH | — | 0.793 | 1.5 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant N106A, NADH |
| azoreductase AzoAvariant N121A | Enterococcus faecalis | Methyl Red | 111 | 0.0082 | 1.354 × 10⁴ | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant N121A, Methyl Red |
| azoreductase AzoAvariant N121A | Enterococcus faecalis | NADH | — | 0.292 | 380 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant N121A, NADH |
| azoreductase AzoAvariant R18G | Enterococcus faecalis | Methyl Red | 3.17 | 0.0167 | 190 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant R18G, Methyl Red |
| azoreductase AzoAvariant R18G | Enterococcus faecalis | NADH | — | 0.624 | 5 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant R18G, NADH |
| azoreductase AzoAvariant R21G | Enterococcus faecalis | Methyl Red | 140 | 0.0126 | 1.111 × 10⁴ | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant R21G, Methyl Red |
| azoreductase AzoAvariant R21G | Enterococcus faecalis | NADH | — | 0.38 | 370 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant R21G, NADH |
| azoreductase AzoAvariant R66A | Enterococcus faecalis | Methyl Red | 77.3 | 0.0053 | 1.458 × 10⁴ | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant R66A, Methyl Red |
| azoreductase AzoAvariant R66A | Enterococcus faecalis | NADH | — | 0.581 | 130 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant R66A, NADH |
| azoreductase AzoAvariant V122Y | Enterococcus faecalis | Methyl Red | 17.5 | 0.1092 | 160 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant V122Y, Methyl Red |
| azoreductase AzoAvariant V122Y | Enterococcus faecalis | NADH | — | 0.405 | 44 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant V122Y, NADH |
| azoreductase AzoAvariant W62A | Enterococcus faecalis | Methyl Red | 21.1 | 0.0335 | 630 | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant W62A, Methyl Red |
| azoreductase AzoAvariant W62A | Enterococcus faecalis | NADH | — | 0.003 | 7.03 × 10³ | pH 7 | PMID 22387379 | Open record: azoreductase AzoA variant W62A, NADH |