CatLogenzyme kinetics catalog

EC 2.4.1.22: lactose synthase

TransferasesOpen in Browse

CatLog read 27 records for EC 2.4.1.22 from 3 papers, for 3 organisms. Names in these papers: β4Gal-T1 (β-1,4 galactosyltransferase 1), β1,4-galactosyltransferase-I (h-β4Gal-T1), UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase (bovine milk GalT), UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase (rat liver Golgi GalT) and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 4 records for EC 2.4.1.22 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.1.22 in Browse.

Papers

PaperOrganismsRecords
Molecular Mechanisms Underlying the Loop-Closing Dynamics of β-1,4 Galactosyltransferase 1Homo sapiens11
Oligosaccharide preferences of beta1,4-galactosyltransferase-I: crystal structures of Met340His mutant of human beta1,4-galactosyltransferase-I with a pentasaccharide and trisaccharides of the…Homo sapiens10
A kinetic comparison of partially purified rat liver Golgi and rat serum galactosyltransferasesBos taurus, Rattus norvegicus6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase (bovine milk GalT)Bos taurusglucose—2—pH 7.5, 37 °CPMID 6426461Open
UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase (bovine milk GalT)Bos taurusUDP-galactose—0.06—pH 7.5, 37 °CPMID 6426461Open
UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase (rat liver Golgi GalT)Rattus norvegicusglucose—2.3—pH 7.5, 37 °CPMID 6426461Open
UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase (rat liver Golgi GalT)Rattus norvegicusUDP-galactose—0.023—pH 7.5, 37 °CPMID 6426461Open
UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase (rat serum GalT)Rattus norvegicusglucose—1.8—pH 7.5, 37 °CPMID 6426461Open
UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase (rat serum GalT)Rattus norvegicusUDP-galactose—0.009—pH 7.5, 37 °CPMID 6426461Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiens1,2-1,3-arm trisaccharide GlcNAcβ1,2-Manα1,3-Manβ-OR, R = linker –CH2–CH2–CH2–CHCH2 (β-linked to reducing-end mannose)2.470.64.09PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiens1,2-1,6-arm trisaccharide GlcNAcβ1,2-Manα1,6-Manβ-OR, R = linker –CH2–CH2–CH2–CHCH2 (β-linked to reducing-end mannose)1.90.0631.66PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiens1,4-1,3-arm trisaccharide GlcNAcβ1,4-Manα1,3-Manβ-OR, R = linker –CH2–CH2–CH2–CHCH2 (β-linked to reducing-end mannose)3.350.714.69PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiensGlcNAcβ1,2-Man4.483.831.16PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiensGlcNAcβ1,2-Manα1,6(GlcNAcβ1,2-Manα1,3)Man1.741.231.41PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiensN,N',N''-triacetylchitotriose5 mM Mn2+; two-substrate kinetic analysis4.811.513.18PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiensN,N',N''-triacetylchitotrioseMn2+ and UDP-Gal at fixed concentrations—0.96—PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiensN,N'-diacetylchitobiose5 mM Mn2+; two-substrate kinetic analysis4.821.513.19PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiensN,N'-diacetylchitobioseMn2+ and UDP-Gal at fixed concentrations—1.59—PMID 16157350Open
β1,4-galactosyltransferase-I (h-β4Gal-T1)Homo sapiensN-acetylglucosamine3.6100.3PMID 16157350Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)Homo sapiensN-acetyl-D-glucosamine7.620.715210.7 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)Homo sapiensuridine diphosphate galactose4.020.03141128 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant D346AHomo sapiensN-acetyl-D-glucosamine2.8328.910.0979 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant D346AHomo sapiensuridine diphosphate galactose0.830.0118770 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant F222AHomo sapiensN-acetyl-D-glucosamine4.4353.20.0832 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant F222AHomo sapiensuridine diphosphate galactose10.18175.5 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant H190AHomo sapiensN-acetyl-D-glucosamine2.3514.510.162 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant H190AHomo sapiensuridine diphosphate galactose0.130.11391.1 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant I341AHomo sapiensN-acetyl-D-glucosamine40.31.09736.7 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant I341AHomo sapiensuridine diphosphate galactose20.190.04589440.0 calc.pH 7.5, 23 °CPMID 39737871Open
β4Gal-T1 (β-1,4 galactosyltransferase 1)variant R187AHomo sapiensN-acetyl-D-glucosamine0.1360.670.0021 calc.pH 7.5, 23 °CPMID 39737871Open