EC 3.1.3.5: 5'-nucleotidase

HydrolasesOpen in Browse

CatLog read 151 records for EC 3.1.3.5 from 16 papers, for 9 organisms. Names in these papers: cytosolic 5'-nucleotidase II (cN-II), 5'-nucleotidase, cytosol 5'-nucleotidase, cytoplasmic 5'-nucleotidase and 16 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 86 records for EC 3.1.3.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.3.5 in Browse.

Papers

Papers with records for EC 3.1.3.5
Paper	Organisms	Records
Mechanistic studies on bovine cytosolic 5'-nucleotidase II, an enzyme belonging to the HAD superfamily	Bos taurus	20
Biochemical and mass spectrometric characterization of soluble ecto-5'-nucleotidase from bull seminal plasma	Bos taurus (bull)	16
Purification and some properties of cytosol 5'-nucleotidase from rat liver	Rattus norvegicus	14
Identification of Drosophila and human 7-methyl GMP-specific nucleotidases	Drosophila melanogaster, Homo sapiens	13
Human placental cytoplasmic 5'-nucleotidase. Kinetic properties and inhibition	Homo sapiens	12
Purification and properties of bovine liver plasma membrane 5' nucleotidase	Bos taurus	10
myo-Inositol-1-Phosphatase from the Pollen of Lilium longiflorum Thunb	Lilium longiflorum	9
Expression and purification of the 5'-nucleotidase YitU from Bacillus species: its enzymatic properties and possible applications in biotechnology	Bacillus subtilis	9
Phosphorylation of ribavirin and viramidine by adenosine kinase and cytosolic 5'-nucleotidase II: Implications for ribavirin metabolism in erythrocytes	Homo sapiens	8
Structural basis of substrate specificity and selectivity of murine cytosolic 5'-nucleotidase III	Mus musculus	8
Crystal structure of a soluble form of human CD73 with ecto-5'-nucleotidase activity	Homo sapiens	8
Contribution of the two domains of E. coli 5'-nucleotidase to substrate specificity and catalysis	Escherichia coli	8
A large hinge bending domain rotation is necessary for the catalytic function of Escherichia coli 5'-nucleotidase	Escherichia coli	5
Identification, Heterologous Expression, and Functional Characterization of Bacillus subtilis YutF, a HAD Superfamily 5'-Nucleotidase with Broad Substrate Specificity	Bacillus subtilis	5
Identification of UshA as a major enzyme for NAD degradation in Escherichia coli	Escherichia coli	4
Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family	Escherichia coli	2

Records

Records for EC 3.1.3.5 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
5'-nucleotidase	Bos taurus	adenosine 5'-monophosphate	—	0.0987	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, adenosine 5'-monophosphate
5'-nucleotidase	Bos taurus	adenosine 5'-monophosphate	—	0.0153	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, adenosine 5'-monophosphate
5'-nucleotidase	Bos taurus	cytidine 5'-monophosphate	—	0.111	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, cytidine 5'-monophosphate
5'-nucleotidase	Bos taurus	cytidine 5'-monophosphate	—	0.0631	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, cytidine 5'-monophosphate
5'-nucleotidase	Bos taurus	guanosine 5'-monophosphate	—	0.0712	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, guanosine 5'-monophosphate
5'-nucleotidase	Bos taurus	guanosine 5'-monophosphate	—	0.0416	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, guanosine 5'-monophosphate
5'-nucleotidase	Bos taurus	inosine 5'-monophosphate	—	0.116	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, inosine 5'-monophosphate
5'-nucleotidase	Bos taurus	inosine 5'-monophosphate	—	0.0519	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, inosine 5'-monophosphate
5'-nucleotidase	Bos taurus	uridine 5'-monophosphate	—	0.0918	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, uridine 5'-monophosphate
5'-nucleotidase	Bos taurus	uridine 5'-monophosphate	—	0.0745	—	pH 7.4, 37 °C	PMID 6317380	Open record: 5'-nucleotidase, uridine 5'-monophosphate
5'-nucleotidase	Escherichia coli	adenosine 5'-monophosphateTable 1	750	0.074	1.0 × 10⁴ calc.	pH 7.5, 25 °C	PMID 15709736	Open record: 5'-nucleotidase, adenosine 5'-monophosphate
5'-nucleotidase	Escherichia coli	adenosine 5'-monophosphatein the text	—	0.052	—	pH 7.5, 25 °C	PMID 15709736	Open record: 5'-nucleotidase, adenosine 5'-monophosphate
5'-nucleotidase	Escherichia coli	para-nitrophenyl phosphate	60	4.741	13 calc.	pH 7.5, 25 °C	PMID 15709736	Open record: 5'-nucleotidase, para-nitrophenyl phosphate
5'-nucleotidasevariant P90C/L424C	Escherichia coli	adenosine 5'-monophosphate	313	0.011	2.8 × 10⁴ calc.	pH 7.5, 25 °C	PMID 15709736	Open record: 5'-nucleotidase variant P90C/L424C, adenosine 5'-monophosphate
5'-nucleotidasevariant P90C/L424C	Escherichia coli	para-nitrophenyl phosphate	350	5.355	65 calc.	pH 7.5, 25 °C	PMID 15709736	Open record: 5'-nucleotidase variant P90C/L424C, para-nitrophenyl phosphate
5'-nucleotidase (5NT)	Escherichia coli	adenosine 5'-diphosphate	613.2	0.0547	1.122 × 10⁴	pH 7	PMID 23333297	Open record: 5'-nucleotidase (5NT), adenosine 5'-diphosphate
5'-nucleotidase (5NT)	Escherichia coli	adenosine 5'-monophosphate	459.1	0.01291	3.56 × 10⁴	pH 7	PMID 23333297	Open record: 5'-nucleotidase (5NT), adenosine 5'-monophosphate
5'-nucleotidase (5NT)	Escherichia coli	adenosine 5'-triphosphate	757	0.0209	3.62 × 10⁴	pH 7	PMID 23333297	Open record: 5'-nucleotidase (5NT), adenosine 5'-triphosphate
5'-nucleotidase (5NT)	Escherichia coli	p-nitrophenyl phosphate	40.8	5.713	7.1	pH 7	PMID 23333297	Open record: 5'-nucleotidase (5NT), p-nitrophenyl phosphate
5'-nucleotidase (5NT)variant 5NT–NDom	Escherichia coli	adenosine 5'-diphosphate	45.6	10.15	4.5	pH 7	PMID 23333297	Open record: 5'-nucleotidase (5NT) variant 5NT–NDom, adenosine 5'-diphosphate
5'-nucleotidase (5NT)variant 5NT–NDom	Escherichia coli	adenosine 5'-monophosphate	—	—	2.89 × 10⁻⁴	pH 7	PMID 23333297	Open record: 5'-nucleotidase (5NT) variant 5NT–NDom, adenosine 5'-monophosphate
5'-nucleotidase (5NT)variant 5NT–NDom	Escherichia coli	adenosine 5'-triphosphate	36.5	6.807	5.4	pH 7	PMID 23333297	Open record: 5'-nucleotidase (5NT) variant 5NT–NDom, adenosine 5'-triphosphate
5'-nucleotidase (5NT)variant 5NT–NDom	Escherichia coli	p-nitrophenyl phosphate	129	21.17	6.1	pH 7	PMID 23333297	Open record: 5'-nucleotidase (5NT) variant 5NT–NDom, p-nitrophenyl phosphate
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	2'-deoxyadenosine 5'-monophosphate	26.25	8.24	3.19	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), 2'-deoxyadenosine 5'-monophosphate
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	2'-deoxyguanosine 5'-monophosphate	13.8	5.61	2.46	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), 2'-deoxyguanosine 5'-monophosphate
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate	11.28	—	—	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	adenosine 5'-monophosphate	28.16	14.32	1.97	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), adenosine 5'-monophosphate
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	cytidine 5'-monophosphate	39.18	17.45	2.25	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), cytidine 5'-monophosphate
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	flavin mononucleotide	22.97	0.096	239	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), flavin mononucleotide
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	guanosine 5'-monophosphate	21.26	7	3.04	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), guanosine 5'-monophosphate
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	inosine 5'-monophosphate	26.34	17.81	1.48	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), inosine 5'-monophosphate
5'-nucleotidase YitU (Ht-YitU_Bs)	Bacillus subtilis	xanthosine 5'-monophosphate	37.49	21.74	1.72	pH 7, 30 °C	PMID 32040605	Open record: 5'-nucleotidase YitU (Ht-YitU_Bs), xanthosine 5'-monophosphate
CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like)	Drosophila melanogaster	7-methylguanosine 5'-monophosphate	6.3	0.013	460	pH 7.5, 25 °C	PMID 23223233	Open record: CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like), 7-methylguanosine 5'-monophosphate
CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like)	Drosophila melanogaster	adenosine 5'-monophosphate	0.28	0.032	10	pH 7.5, 25 °C	PMID 23223233	Open record: CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like), adenosine 5'-monophosphate
CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like)	Drosophila melanogaster	cytidine 5'-monophosphate	12	0.048	250	pH 7.5, 25 °C	PMID 23223233	Open record: CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like), cytidine 5'-monophosphate
CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like)	Drosophila melanogaster	guanosine 5'-monophosphate	0.88	0.102	10	pH 7.5, 25 °C	PMID 23223233	Open record: CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like), guanosine 5'-monophosphate
CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like)	Drosophila melanogaster	uridine 5'-monophosphate	2.3	0.091	30	pH 7.5, 25 °C	PMID 23223233	Open record: CG3362 5' nucleotidase (m7GMP-specific; Drosophila cNIII-like), uridine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	2'-deoxyadenosine 5'-monophosphate	—	0.035	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, 2'-deoxyadenosine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	2'-deoxycytidine 5'-monophosphate	—	0.023	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, 2'-deoxycytidine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	2'-deoxyguanosine 5'-monophosphate	—	0.044	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, 2'-deoxyguanosine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	2'-deoxyinosine 5'-monophosphate	—	0.057	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, 2'-deoxyinosine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	2'-deoxyuridine 5'-monophosphate	—	0.017	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, 2'-deoxyuridine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	adenosine 5'-monophosphate20 mM MgCl2; 20 min incubation	—	0.018	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, adenosine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	adenosine 5'-monophosphateAMP varied at fixed MgCl2 0.1-5.1 mM	—	0.014	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, adenosine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	cytidine 5'-monophosphate	—	0.002	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, cytidine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	guanosine 5'-monophosphate	—	0.017	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, guanosine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	inosine 5'-monophosphate	—	0.03	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, inosine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	uridine 5'-monophosphate	—	0.014	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, uridine 5'-monophosphate
cytoplasmic 5'-nucleotidase	Homo sapiens	xanthosine 5'-monophosphate	—	0.065	—	pH 7.4, 37 °C	PMID 3001058	Open record: cytoplasmic 5'-nucleotidase, xanthosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	2'-deoxyadenosine 5'-monophosphate	—	35	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, 2'-deoxyadenosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	2'-deoxyguanosine 5'-monophosphate	—	1.1	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, 2'-deoxyguanosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	2'-deoxyinosine 5'-monophosphate	—	0.7	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, 2'-deoxyinosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	adenosine 5'-monophosphate	—	14	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, adenosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	cytidine 5'-monophosphate	—	17	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, cytidine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	guanosine 5'-monophosphate500 mM NaCl; V and Km by direct linear plot	—	0.7	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, guanosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	guanosine 5'-monophosphateno NaCl	—	2.1	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, guanosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	guanosine 5'-monophosphateno NaCl; with 5 mM ATP	—	0.3	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, guanosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	inosine 5'-monophosphate500 mM NaCl; V and Km by direct linear plot	—	0.2	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, inosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	inosine 5'-monophosphateno NaCl; Km by direct linear plot	—	1	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, inosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	inosine 5'-monophosphate150 mM Tris-maleate (pH 6.5); Km by direct linear plot	—	0.2	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, inosine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	thymidine 5'-monophosphate	—	22	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, thymidine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	uridine 5'-monophosphate	—	6	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, uridine 5'-monophosphate
cytosol 5'-nucleotidase	Rattus norvegicus	xanthosine 5'-monophosphate	—	2.9	—	pH 6.5, 37 °C	PMID 6260203	Open record: cytosol 5'-nucleotidase, xanthosine 5'-monophosphate
cytosolic 5' nucleotidase III (cNIII)	Homo sapiens	cytidine 5'-monophosphate	—	0.03	—	pH 7.5, 37 °C	PMID 23223233	Open record: cytosolic 5' nucleotidase III (cNIII), cytidine 5'-monophosphate
cytosolic 5' nucleotidase III-like (cNIII-like)	Homo sapiens	7-methylguanosine 5'-monophosphate	0.24	0.0078	30	pH 7.5, 37 °C	PMID 23223233	Open record: cytosolic 5' nucleotidase III-like (cNIII-like), 7-methylguanosine 5'-monophosphate
cytosolic 5' nucleotidase III-like (cNIII-like)	Homo sapiens	adenosine 5'-monophosphate	0.04	0.456	0.1	pH 7.5, 37 °C	PMID 23223233	Open record: cytosolic 5' nucleotidase III-like (cNIII-like), adenosine 5'-monophosphate
cytosolic 5' nucleotidase III-like (cNIII-like)	Homo sapiens	cytidine 5'-monophosphate	7	0.079	90	pH 7.5, 37 °C	PMID 23223233	Open record: cytosolic 5' nucleotidase III-like (cNIII-like), cytidine 5'-monophosphate
cytosolic 5' nucleotidase III-like (cNIII-like)	Homo sapiens	guanosine 5'-monophosphate	0.07	0.355	0.2	pH 7.5, 37 °C	PMID 23223233	Open record: cytosolic 5' nucleotidase III-like (cNIII-like), guanosine 5'-monophosphate
cytosolic 5' nucleotidase III-like (cNIII-like)	Homo sapiens	uridine 5'-monophosphate	6.2	0.439	10	pH 7.5, 37 °C	PMID 23223233	Open record: cytosolic 5' nucleotidase III-like (cNIII-like), uridine 5'-monophosphate
cytosolic 5'-nucleotidase II (cN-II)	Bos taurus	inosine	40	1.1	36 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II), inosine
cytosolic 5'-nucleotidase II (cN-II)	Bos taurus	inosine monophosphate	—	0.1	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II), inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)variant D351E	Bos taurus	inosine	—	0.9	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant D351E, inosine
cytosolic 5'-nucleotidase II (cN-II)variant D356E	Bos taurus	inosine	2.2	1	2.2 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant D356E, inosine
cytosolic 5'-nucleotidase II (cN-II)variant D356E	Bos taurus	inosine monophosphate	—	0.2	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant D356E, inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)variant D356N	Bos taurus	inosine	1.4	10.2	0.14 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant D356N, inosine
cytosolic 5'-nucleotidase II (cN-II)variant D356N	Bos taurus	inosine monophosphate	—	0.2	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant D356N, inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)variant K292M	Bos taurus	inosine	—	0.5	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant K292M, inosine
cytosolic 5'-nucleotidase II (cN-II)variant M53I	Bos taurus	inosine	3.7	0.8	4.6 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant M53I, inosine
cytosolic 5'-nucleotidase II (cN-II)variant M53I	Bos taurus	inosine monophosphate	—	1	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant M53I, inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)variant M53N	Bos taurus	inosine	0.6	1.1	0.55 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant M53N, inosine
cytosolic 5'-nucleotidase II (cN-II)variant M53N	Bos taurus	inosine monophosphate	—	0.1	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant M53N, inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)variant S251A	Bos taurus	inosine	12.3	1	12 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant S251A, inosine
cytosolic 5'-nucleotidase II (cN-II)variant S251A	Bos taurus	inosine monophosphate	—	0.1	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant S251A, inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)variant S251T	Bos taurus	inosine	3.4	1.5	2.3 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant S251T, inosine
cytosolic 5'-nucleotidase II (cN-II)variant S251T	Bos taurus	inosine monophosphate	—	0.3	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant S251T, inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)variant T249S	Bos taurus	inosine	19.8	0.9	22 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant T249S, inosine
cytosolic 5'-nucleotidase II (cN-II)variant T249S	Bos taurus	inosine monophosphate	—	0.2	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant T249S, inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)variant T249V	Bos taurus	inosine	0.3	0.25	1.2 calc.		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant T249V, inosine
cytosolic 5'-nucleotidase II (cN-II)variant T249V	Bos taurus	inosine monophosphate	—	0.4	—		PMID 15606776	Open record: cytosolic 5'-nucleotidase II (cN-II) variant T249V, inosine monophosphate
cytosolic 5'-nucleotidase II (cN-II)	Homo sapiens	1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide2 mM ATP	0.157	0.095	1.65	pH 7.4, 37 °C	PMID 15917509	Open record: cytosolic 5'-nucleotidase II (cN-II), 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide
cytosolic 5'-nucleotidase II (cN-II)	Homo sapiens	1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide0.4 mM BPG (2,3-bisphosphoglycerate)	0.027	0.047	0.58	pH 7.4, 37 °C	PMID 15917509	Open record: cytosolic 5'-nucleotidase II (cN-II), 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide
cytosolic 5'-nucleotidase II (cN-II)	Homo sapiens	1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide2 mM ATP as activator; no BPG	0.023	0.024	0.97	37 °C	PMID 15917509	Open record: cytosolic 5'-nucleotidase II (cN-II), 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide
cytosolic 5'-nucleotidase II (cN-II)	Homo sapiens	1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide	0.067	0.088	0.75	37 °C	PMID 15917509	Open record: cytosolic 5'-nucleotidase II (cN-II), 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide
cytosolic 5'-nucleotidase II (cN-II)	Homo sapiens	1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamidine2 mM ATP	—	—	0.103	pH 7.4, 37 °C	PMID 15917509	Open record: cytosolic 5'-nucleotidase II (cN-II), 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamidine
cytosolic 5'-nucleotidase II (cN-II)	Homo sapiens	1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamidine0.4 mM BPG (2,3-bisphosphoglycerate)	—	—	0.042	pH 7.4, 37 °C	PMID 15917509	Open record: cytosolic 5'-nucleotidase II (cN-II), 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamidine
cytosolic 5'-nucleotidase II (cN-II)	Homo sapiens	inosine2 mM ATP	21.8	2.17	10.2	pH 7.4, 37 °C	PMID 15917509	Open record: cytosolic 5'-nucleotidase II (cN-II), inosine
cytosolic 5'-nucleotidase II (cN-II)	Homo sapiens	inosine0.4 mM BPG (2,3-bisphosphoglycerate)	19.3	1.84	10.5	pH 7.4, 37 °C	PMID 15917509	Open record: cytosolic 5'-nucleotidase II (cN-II), inosine
cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1)	Mus musculus	uridine 5'-monophosphate	1.2	1.1	1.1	pH 7.5, 37 °C	PMID 22925580	Open record: cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1), uridine 5'-monophosphate
cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1)variant E96S	Mus musculus	uridine 5'-monophosphate	0.38	4.8	0.08	pH 7.5, 37 °C	PMID 22925580	Open record: cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1) variant E96S, uridine 5'-monophosphate
cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1)variant H68A	Mus musculus	uridine 5'-monophosphate	0.12	3.2	0.037	pH 7.5, 37 °C	PMID 22925580	Open record: cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1) variant H68A, uridine 5'-monophosphate
cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1)variant N69G	Mus musculus	uridine 5'-monophosphate	1.1	2.1	0.55	pH 7.5, 37 °C	PMID 22925580	Open record: cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1) variant N69G, uridine 5'-monophosphate
cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1)variant S117G	Mus musculus	uridine 5'-monophosphate	1.7	1.1	1.5	pH 7.5, 37 °C	PMID 22925580	Open record: cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1) variant S117G, uridine 5'-monophosphate
cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1)variant T66G	Mus musculus	uridine 5'-monophosphate	1.9	1.1	1.7	pH 7.5, 37 °C	PMID 22925580	Open record: cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1) variant T66G, uridine 5'-monophosphate
cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1)variant W113A	Mus musculus	uridine 5'-monophosphate	0.29	29	0.01	pH 7.5, 37 °C	PMID 22925580	Open record: cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1) variant W113A, uridine 5'-monophosphate
cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1)variant Y114A	Mus musculus	uridine 5'-monophosphate	0.14	2.3	0.06	pH 7.5, 37 °C	PMID 22925580	Open record: cytosolic 5'-nucleotidase III (cN-III, pyrimidine 5'-nucleotidase type 1) variant Y114A, uridine 5'-monophosphate
ecto-5'-nucleotidase CD73 (soluble form sCD73)	Homo sapiens	2'-deoxyadenosine 5'-monophosphate	174	0.114	1.5 × 10³	37 °C	PMID 22997138	Open record: ecto-5'-nucleotidase CD73 (soluble form sCD73), 2'-deoxyadenosine 5'-monophosphate
ecto-5'-nucleotidase CD73 (soluble form sCD73)	Homo sapiens	adenosine 5'-monophosphate	187	0.0038	4.88 × 10⁴	37 °C	PMID 22997138	Open record: ecto-5'-nucleotidase CD73 (soluble form sCD73), adenosine 5'-monophosphate
ecto-5'-nucleotidase CD73 (soluble form sCD73)	Homo sapiens	beta-nicotinamide mononucleotide	102	0.88	120	37 °C	PMID 22997138	Open record: ecto-5'-nucleotidase CD73 (soluble form sCD73), beta-nicotinamide mononucleotide
ecto-5'-nucleotidase CD73 (soluble form sCD73)	Homo sapiens	cytidine 5'-monophosphate	202	0.0564	3.6 × 10³	37 °C	PMID 22997138	Open record: ecto-5'-nucleotidase CD73 (soluble form sCD73), cytidine 5'-monophosphate
ecto-5'-nucleotidase CD73 (soluble form sCD73)	Homo sapiens	guanosine 5'-monophosphate	132	0.0065	2.03 × 10⁴	37 °C	PMID 22997138	Open record: ecto-5'-nucleotidase CD73 (soluble form sCD73), guanosine 5'-monophosphate
ecto-5'-nucleotidase CD73 (soluble form sCD73)	Homo sapiens	inosine 5'-monophosphate	104	0.0071	1.47 × 10⁴	37 °C	PMID 22997138	Open record: ecto-5'-nucleotidase CD73 (soluble form sCD73), inosine 5'-monophosphate
ecto-5'-nucleotidase CD73 (soluble form sCD73)	Homo sapiens	thymidine 5'-monophosphate	40	0.008	5 × 10³	37 °C	PMID 22997138	Open record: ecto-5'-nucleotidase CD73 (soluble form sCD73), thymidine 5'-monophosphate
ecto-5'-nucleotidase CD73 (soluble form sCD73)	Homo sapiens	uridine 5'-monophosphate	289	0.0316	9.1 × 10³	37 °C	PMID 22997138	Open record: ecto-5'-nucleotidase CD73 (soluble form sCD73), uridine 5'-monophosphate
m7GMP-specific 5' nucleotidase (partially purified; later identified as the CG3362 product)	Drosophila melanogaster	7-methylguanosine 5'-monophosphate	—	0.004	—	pH 7.5, 25 °C	PMID 23223233	Open record: m7GMP-specific 5' nucleotidase (partially purified; later identified as the CG3362 product), 7-methylguanosine 5'-monophosphate
m7GMP-specific 5' nucleotidase (partially purified; later identified as the CG3362 product)	Drosophila melanogaster	guanosine 5'-monophosphate	—	0.1	—	pH 7.5, 25 °C	PMID 23223233	Open record: m7GMP-specific 5' nucleotidase (partially purified; later identified as the CG3362 product), guanosine 5'-monophosphate
myo-inositol-1-phosphatase	Lilium longiflorum	1D-myo-inositol 1-phosphate	—	0.078	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, 1D-myo-inositol 1-phosphate
myo-inositol-1-phosphatase	Lilium longiflorum	1L-myo-inositol 1-phosphate	—	0.082	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, 1L-myo-inositol 1-phosphate
myo-inositol-1-phosphatase	Lilium longiflorum	adenosine 2'-monophosphate	—	1.58	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, adenosine 2'-monophosphate
myo-inositol-1-phosphatase	Lilium longiflorum	adenosine 3'-monophosphate	—	0.372	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, adenosine 3'-monophosphate
myo-inositol-1-phosphatase	Lilium longiflorum	beta-glycerophosphate (glycerol 2-phosphate)	—	0.061	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, beta-glycerophosphate (glycerol 2-phosphate)
myo-inositol-1-phosphatase	Lilium longiflorum	D-glucose 6-phosphate	—	1.71	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, D-glucose 6-phosphate
myo-inositol-1-phosphatase	Lilium longiflorum	DL-alpha-glycerophosphate (rac-glycerol 1-phosphate)	—	0.382	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, DL-alpha-glycerophosphate (rac-glycerol 1-phosphate)
myo-inositol-1-phosphatase	Lilium longiflorum	L-alpha-glycerophosphate	—	0.562	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, L-alpha-glycerophosphate
myo-inositol-1-phosphatase	Lilium longiflorum	myo-inositol 2-phosphate	—	0.733	—	pH 8.5, 37 °C	PMID 16662572	Open record: myo-inositol-1-phosphatase, myo-inositol 2-phosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-1	Bos taurus (bull)	2'-deoxycytidine 5'-monophosphate	—	0.05	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-1, 2'-deoxycytidine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-1	Bos taurus (bull)	adenosine 5'-monophosphate	—	0.11	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-1, adenosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-1	Bos taurus (bull)	guanosine 5'-monophosphate	—	0.22	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-1, guanosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-1	Bos taurus (bull)	inosine 5'-monophosphate	—	0.15	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-1, inosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-2	Bos taurus (bull)	2'-deoxycytidine 5'-monophosphate	—	0.04	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-2, 2'-deoxycytidine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-2	Bos taurus (bull)	adenosine 5'-monophosphate	—	0.06	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-2, adenosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-2	Bos taurus (bull)	guanosine 5'-monophosphate	—	0.09	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-2, guanosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-2	Bos taurus (bull)	inosine 5'-monophosphate	—	0.07	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-2, inosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-3	Bos taurus (bull)	2'-deoxycytidine 5'-monophosphate	—	0.03	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-3, 2'-deoxycytidine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-3	Bos taurus (bull)	adenosine 5'-monophosphate	—	0.11	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-3, adenosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-3	Bos taurus (bull)	guanosine 5'-monophosphate	—	0.16	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-3, guanosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-3	Bos taurus (bull)	inosine 5'-monophosphate	—	0.06	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-3, inosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-4	Bos taurus (bull)	2'-deoxycytidine 5'-monophosphate	—	0.06	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-4, 2'-deoxycytidine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-4	Bos taurus (bull)	adenosine 5'-monophosphate	—	0.06	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-4, adenosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-4	Bos taurus (bull)	guanosine 5'-monophosphate	—	0.07	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-4, guanosine 5'-monophosphate
soluble ecto-5'-nucleotidase isoform 5'-NT-4	Bos taurus (bull)	inosine 5'-monophosphate	—	0.07	—	37 °C	PMID 12608891	Open record: soluble ecto-5'-nucleotidase isoform 5'-NT-4, inosine 5'-monophosphate
UshA (5'-nucleotidase/UDP-sugar hydrolase, NAD pyrophosphatase)	Escherichia coli	adenosine monophosphate	405	0.0012	3.34 × 10⁵	pH 7.5, 37 °C	PMID 24731828	Open record: UshA (5'-nucleotidase/UDP-sugar hydrolase, NAD pyrophosphatase), adenosine monophosphate
UshA (5'-nucleotidase/UDP-sugar hydrolase, NAD pyrophosphatase)	Escherichia coli	nicotinamide adenine dinucleotide	19.3	0.0053	3.7 × 10³	pH 7.5, 37 °C	PMID 24731828	Open record: UshA (5'-nucleotidase/UDP-sugar hydrolase, NAD pyrophosphatase), nicotinamide adenine dinucleotide
UshA (5'-nucleotidase/UDP-sugar hydrolase, NAD pyrophosphatase)	Escherichia coli	nicotinamide adenine dinucleotide, reduced form	10	0.007	1.4 × 10³	pH 7.5, 37 °C	PMID 24731828	Open record: UshA (5'-nucleotidase/UDP-sugar hydrolase, NAD pyrophosphatase), nicotinamide adenine dinucleotide, reduced form
UshA (5'-nucleotidase/UDP-sugar hydrolase, NAD pyrophosphatase)	Escherichia coli	nicotinamide mononucleotide	1492	0.0111	1.35 × 10⁵	pH 7.5, 37 °C	PMID 24731828	Open record: UshA (5'-nucleotidase/UDP-sugar hydrolase, NAD pyrophosphatase), nicotinamide mononucleotide
YrfG (HAD8)	Escherichia coli	GMP	5.5	1.9	2.9		PMID 16990279	Open record: YrfG (HAD8), GMP
YrfG (HAD8)	Escherichia coli	IMP	2.6	1.2	2.3		PMID 16990279	Open record: YrfG (HAD8), IMP
YutF (5'-nucleotidase)	Bacillus subtilis	5-phosphoribosyl-1-pyrophosphate	0.17	1.27	0.13	pH 6, 37 °C	PMID 27907199	Open record: YutF (5'-nucleotidase), 5-phosphoribosyl-1-pyrophosphate
YutF (5'-nucleotidase)	Bacillus subtilis	guanosine 5'-monophosphate	0.06	6.65	0.009	pH 6, 37 °C	PMID 27907199	Open record: YutF (5'-nucleotidase), guanosine 5'-monophosphate
YutF (5'-nucleotidase)	Bacillus subtilis	p-nitrophenyl phosphate	61	1.64	37	pH 8.9, 25 °C	PMID 27907199	Open record: YutF (5'-nucleotidase), p-nitrophenyl phosphate
YutF (5'-nucleotidase)	Bacillus subtilis	ribose-5-phosphate	1.84	24	0.077	pH 6, 37 °C	PMID 27907199	Open record: YutF (5'-nucleotidase), ribose-5-phosphate
YutF (5'-nucleotidase)	Bacillus subtilis	xanthosine 5'-monophosphate	0.31	1.53	0.21	pH 6, 37 °C	PMID 27907199	Open record: YutF (5'-nucleotidase), xanthosine 5'-monophosphate