EC 3.1.3.8: 3-phytase
HydrolasesOpen in Browse
CatLog read 65 records for EC 3.1.3.8 from 7 papers, for 7 organisms. Names in these papers: β-propeller phytase (BPP), phytase (3-phytase), phytase (PhyA), alkaline phytase phy168 and 7 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 135 records for EC 3.1.3.8 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.3.8 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Characterization and application of calcium-dependent β-propeller phytase from Bacillus amyloliquefaciens DS11 | Bacillus amyloliquefaciens | 18 |
| Purification and characterization of a phytase from Klebsiella terrigena | Klebsiella terrigena | 13 |
| Improved thermostability and enzyme activity of a recombinant phyA mutant phytase from Aspergillus niger N25 by directed evolution and site-directed mutagenesis | Aspergillus niger | 8 |
| doi:10.1016/j.bej.2015.02.021 | Bacillus subtilis | 8 |
| The tandemly repeated domains of a β-propeller phytase act synergistically to increase catalytic efficiency | Bacillus sp., Bacillus subtilis and 1 more | 7 |
| Expression and characterization of Aspergillus thermostable phytases in Pichia pastoris | Aspergillus japonicus, Aspergillus niger | 6 |
| Site-directed mutagenesis of disulfide bridges in Aspergillus niger NRRL 3135 phytase (PhyA), their expression in Pichia pastoris and catalytic characterization | Aspergillus niger | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| 168PhyA (beta-propeller phytase) | Bacillus subtilis | myo-inositol hexakisphosphate | 5.92 | 0.24 | 250 | 55 °C | PMID 21707924 | Open record: 168PhyA (beta-propeller phytase), myo-inositol hexakisphosphate |
| alkaline phytase phy168 | Bacillus subtilis | myo-inositol hexakisphosphate | 4966 | 2.19 | 2268 | pH 7, 37 °C | doi:10.1016/j.bej.2015.02.021 | Open record: alkaline phytase phy168, myo-inositol hexakisphosphate |
| alkaline phytase phy168 | Bacillus subtilis | myo-inositol hexakisphosphate | 1838 | 0.78 | 2368 | pH 4.5, 37 °C | doi:10.1016/j.bej.2015.02.021 | Open record: alkaline phytase phy168, myo-inositol hexakisphosphate |
| alkaline phytase phy168variant D24G | Bacillus subtilis | myo-inositol hexakisphosphate | 7368 | 1.4 | 5259 | pH 7, 37 °C | doi:10.1016/j.bej.2015.02.021 | Open record: alkaline phytase phy168 variant D24G, myo-inositol hexakisphosphate |
| alkaline phytase phy168variant D24G | Bacillus subtilis | myo-inositol hexakisphosphate | 2528 | 0.54 | 4696 | pH 4.5, 37 °C | doi:10.1016/j.bej.2015.02.021 | Open record: alkaline phytase phy168 variant D24G, myo-inositol hexakisphosphate |
| alkaline phytase phy168variant D24G/K265N | Bacillus subtilis | myo-inositol hexakisphosphate | 8430 | 1.77 | 4767 | pH 7, 37 °C | doi:10.1016/j.bej.2015.02.021 | Open record: alkaline phytase phy168 variant D24G/K265N, myo-inositol hexakisphosphate |
| alkaline phytase phy168variant D24G/K265N | Bacillus subtilis | myo-inositol hexakisphosphate | 2510 | 0.49 | 5080 | pH 4.5, 37 °C | doi:10.1016/j.bej.2015.02.021 | Open record: alkaline phytase phy168 variant D24G/K265N, myo-inositol hexakisphosphate |
| alkaline phytase phy168variant D24G/K70R/K111E/N121S | Bacillus subtilis | myo-inositol hexakisphosphate | 7.86 × 10³ | 1.5 | 5232 | pH 7, 37 °C | doi:10.1016/j.bej.2015.02.021 | Open record: alkaline phytase phy168 variant D24G/K70R/K111E/N121S, myo-inositol hexakisphosphate |
| alkaline phytase phy168variant D24G/K70R/K111E/N121S | Bacillus subtilis | myo-inositol hexakisphosphate | 2451 | 0.39 | 6236 | pH 4.5, 37 °C | doi:10.1016/j.bej.2015.02.021 | Open record: alkaline phytase phy168 variant D24G/K70R/K111E/N121S, myo-inositol hexakisphosphate |
| PhyH (dual-domain beta-propeller phytase) | Bacillus sp. | myo-inositol hexakisphosphate | 27.72 | 0.5 | 54 | pH 7, 35 °C | PMID 21707924 | Open record: PhyH (dual-domain beta-propeller phytase), myo-inositol hexakisphosphate |
| PhyH (dual-domain beta-propeller phytase) | Bacillus sp. | myo-inositol hexakisphosphate | 17.76 | 1.143 | 15.5 | pH 7, 20 °C | PMID 21707924 | Open record: PhyH (dual-domain beta-propeller phytase), myo-inositol hexakisphosphate |
| PhyH (dual-domain beta-propeller phytase)PhyH-DII (C-terminal domain, residues 319-644) | Bacillus sp. | myo-inositol hexakisphosphate | 4.17 | 1.432 | 2.9 | pH 7, 35 °C | PMID 21707924 | Open record: PhyH (dual-domain beta-propeller phytase) PhyH-DII (C-terminal domain, residues 319-644), myo-inositol hexakisphosphate |
| PhyH-DI-168PhyA fusion phytasePhyH-DI-168PhyA fusion | — | myo-inositol hexakisphosphate | 12.28 | 0.191 | 640 | pH 7, 55 °C | PMID 21707924 | Open record: PhyH-DI-168PhyA fusion phytase PhyH-DI-168PhyA fusion, myo-inositol hexakisphosphate |
| PhyH-DI-PhyP fusion phytasePhyH-DI-PhyP fusion | — | myo-inositol hexakisphosphate | 94.4 | 1.086 | 87 | pH 7, 37 °C | PMID 21707924 | Open record: PhyH-DI-PhyP fusion phytase PhyH-DI-PhyP fusion, myo-inositol hexakisphosphate |
| PhyP (beta-propeller phytase) | Pedobacter nyackensis | myo-inositol hexakisphosphate | 44.94 | 1.28 | 35 | 37 °C | PMID 21707924 | Open record: PhyP (beta-propeller phytase), myo-inositol hexakisphosphate |
| phytase (3-phytase) | Klebsiella terrigena | 1-Naphthyl phosphate | 2 | 250 | 0.008 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), 1-Naphthyl phosphate |
| phytase (3-phytase) | Klebsiella terrigena | 2-Glycerophosphate | 4 | 110 | 0.036 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), 2-Glycerophosphate |
| phytase (3-phytase) | Klebsiella terrigena | 2-Naphthyl phosphate | 6 | 230 | 0.026 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), 2-Naphthyl phosphate |
| phytase (3-phytase) | Klebsiella terrigena | 4-nitrophenyl phosphate | 20 | 120 | 0.17 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), 4-nitrophenyl phosphate |
| phytase (3-phytase) | Klebsiella terrigena | adenosine 5'-diphosphate | 9 | 90 | 0.1 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), adenosine 5'-diphosphate |
| phytase (3-phytase) | Klebsiella terrigena | adenosine 5'-monophosphate | 8 | 95 | 0.084 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), adenosine 5'-monophosphate |
| phytase (3-phytase) | Klebsiella terrigena | adenosine 5'-triphosphate | 6 | 55 | 0.109 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), adenosine 5'-triphosphate |
| phytase (3-phytase) | Klebsiella terrigena | disodium dihydrogen pyrophosphate | 11 | 78 | 0.141 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), disodium dihydrogen pyrophosphate |
| phytase (3-phytase) | Klebsiella terrigena | Fructose 1,6-diphosphate | 10 | 18 | 0.555 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), Fructose 1,6-diphosphate |
| phytase (3-phytase) | Klebsiella terrigena | Fructose 6-phosphate | 6 | 56 | 0.107 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), Fructose 6-phosphate |
| phytase (3-phytase) | Klebsiella terrigena | Glucose 6-phosphate | 2 | 20 | 0.1 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), Glucose 6-phosphate |
| phytase (3-phytase) | Klebsiella terrigena | myo-inositol (1,2,3,4,5,6)-hexakisphosphate (sodium salt) | 180 | 0.3 | 600 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), myo-inositol (1,2,3,4,5,6)-hexakisphosphate (sodium salt) |
| phytase (3-phytase) | Klebsiella terrigena | NADP+ (nicotinamide adenine dinucleotide phosphate) | 46 | 6 | 7.67 | pH 5, 35 °C | PMID 9169005 | Open record: phytase (3-phytase), NADP+ (nicotinamide adenine dinucleotide phosphate) |
| phytase (PhyA) | Aspergillus niger | myo-inositol hexakisphosphate | 350 | 0.07 | 5 × 10³ | 58 °C | PMID 20376636 | Open record: phytase (PhyA), myo-inositol hexakisphosphate |
| phytase (PhyA)variant C215S | Aspergillus niger | myo-inositol hexakisphosphate | 62 | 0.145 | 430 | 37 °C | PMID 20376636 | Open record: phytase (PhyA) variant C215S, myo-inositol hexakisphosphate |
| phytase (PhyA)variant C264G | Aspergillus niger | myo-inositol hexakisphosphate | 36 | 0.056 | 720 | 37 °C | PMID 20376636 | Open record: phytase (PhyA) variant C264G, myo-inositol hexakisphosphate |
| phytase (PhyA)variant C31G | Aspergillus niger | myo-inositol hexakisphosphate | 234 | 0.18 | 1.3 × 10³ | 53 °C | PMID 20376636 | Open record: phytase (PhyA) variant C31G, myo-inositol hexakisphosphate |
| phytase (PhyA)variant C435G | Aspergillus niger | myo-inositol hexakisphosphate | 90 | 0.045 | 2 × 10³ | 42 °C | PMID 20376636 | Open record: phytase (PhyA) variant C435G, myo-inositol hexakisphosphate |
| phytase (PhyA)variant PP-NP^{ep}-6A (E156G/T236A/Q396R) | Aspergillus niger | inositol hexaphosphoric acid, dodecasodium salt | 0.557 | 0.02769 | 20.08 | PMID 29108630 | Open record: phytase (PhyA) variant PP-NP^{ep}-6A (E156G/T236A/Q396R), inositol hexaphosphoric acid, dodecasodium salt | |
| phytase (PhyA)variant Q172R | Aspergillus niger | inositol hexaphosphoric acid, dodecasodium salt | 0.5758 | 0.01794 | 32.13 | PMID 29108630 | Open record: phytase (PhyA) variant Q172R, inositol hexaphosphoric acid, dodecasodium salt | |
| phytase (PhyA)variant Q172R/K432R | Aspergillus niger | inositol hexaphosphoric acid, dodecasodium salt | 0.5532 | 0.02189 | 25.35 | PMID 29108630 | Open record: phytase (PhyA) variant Q172R/K432R, inositol hexaphosphoric acid, dodecasodium salt | |
| phytase (PhyA)variant Q172R/K432R/Q368E | Aspergillus niger | inositol hexaphosphoric acid, dodecasodium salt | 0.4757 | 0.01927 | 24.63 | PMID 29108630 | Open record: phytase (PhyA) variant Q172R/K432R/Q368E, inositol hexaphosphoric acid, dodecasodium salt | |
| phytase (PhyA)variant Q172R/K432R/Q368E/F376Y | Aspergillus niger | inositol hexaphosphoric acid, dodecasodium salt | 0.7103 | 0.02075 | 34.32 | PMID 29108630 | Open record: phytase (PhyA) variant Q172R/K432R/Q368E/F376Y, inositol hexaphosphoric acid, dodecasodium salt | |
| phytase (PhyA)variant Q172R/K432R/Q368E/F376Y/T195L | Aspergillus niger | inositol hexaphosphoric acid, dodecasodium salt | 0.4957 | 0.03073 | 16 | PMID 29108630 | Open record: phytase (PhyA) variant Q172R/K432R/Q368E/F376Y/T195L, inositol hexaphosphoric acid, dodecasodium salt | |
| phytase (PhyA)variant Q368E/K432R | Aspergillus niger | inositol hexaphosphoric acid, dodecasodium salt | 0.4223 | 0.01073 | 39.53 | PMID 29108630 | Open record: phytase (PhyA) variant Q368E/K432R, inositol hexaphosphoric acid, dodecasodium salt | |
| phytase (PhyA)variant T195L/Q368E/F376Y | Aspergillus niger | inositol hexaphosphoric acid, dodecasodium salt | 0.5477 | 0.01547 | 35.73 | PMID 29108630 | Open record: phytase (PhyA) variant T195L/Q368E/F376Y, inositol hexaphosphoric acid, dodecasodium salt | |
| phytase r-PhyA170 | Aspergillus niger | myo-inositol hexakisphosphate (phytic acid dipotassium salt) | 253.5 | 0.16 | 1584 | pH 5.5, 50 °C | PMID 19025560 | Open record: phytase r-PhyA170, myo-inositol hexakisphosphate (phytic acid dipotassium salt) |
| phytase r-PhyA170 | Aspergillus niger | myo-inositol hexakisphosphate (phytic acid dodecasodium salt hydrate) | 281.2 | 0.15 | 1875 | pH 5.5, 50 °C | PMID 19025560 | Open record: phytase r-PhyA170, myo-inositol hexakisphosphate (phytic acid dodecasodium salt hydrate) |
| phytase r-PhyA170 | Aspergillus niger | p-nitrophenyl phosphate | 2.6 | 63.46 | 0.04 | pH 5.5, 50 °C | PMID 19025560 | Open record: phytase r-PhyA170, p-nitrophenyl phosphate |
| phytase r-PhyA86 | Aspergillus japonicus | myo-inositol hexakisphosphate (phytic acid dipotassium salt) | 279.3 | 0.08 | 3491 | pH 5.5, 50 °C | PMID 19025560 | Open record: phytase r-PhyA86, myo-inositol hexakisphosphate (phytic acid dipotassium salt) |
| phytase r-PhyA86 | Aspergillus japonicus | myo-inositol hexakisphosphate (phytic acid dodecasodium salt hydrate) | 231.2 | 0.14 | 1651 | pH 5.5, 50 °C | PMID 19025560 | Open record: phytase r-PhyA86, myo-inositol hexakisphosphate (phytic acid dodecasodium salt hydrate) |
| phytase r-PhyA86 | Aspergillus japonicus | p-nitrophenyl phosphate | 5.25 | 51.33 | 0.1 | pH 5.5, 50 °C | PMID 19025560 | Open record: phytase r-PhyA86, p-nitrophenyl phosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | 16.55 | 0.138 | 119.9 | pH 7 | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.159 | — | pH 4, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.154 | — | pH 4.5, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.151 | — | pH 5, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.148 | — | pH 5.5, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.146 | — | pH 6, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.137 | — | pH 6.5, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.134 | — | pH 7, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.136 | — | pH 7.5, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.143 | — | pH 8, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.157 | — | pH 8.5, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.163 | — | pH 9, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP) | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | — | 0.174 | — | pH 9.5, 37 °C | PMID 22775008 | Open record: β-propeller phytase (BPP), myo-inositol hexakisphosphate |
| β-propeller phytase (BPP)variant D308A | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | 8.33 | 0.167 | 49.9 | pH 7 | PMID 22775008 | Open record: β-propeller phytase (BPP) variant D308A, myo-inositol hexakisphosphate |
| β-propeller phytase (BPP)variant D308E | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | 15.55 | 0.156 | 99.70 | pH 7 | PMID 22775008 | Open record: β-propeller phytase (BPP) variant D308E, myo-inositol hexakisphosphate |
| β-propeller phytase (BPP)variant D56A | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | 0.067 | 0.248 | 0.2687 | pH 7 | PMID 22775008 | Open record: β-propeller phytase (BPP) variant D56A, myo-inositol hexakisphosphate |
| β-propeller phytase (BPP)variant D56E | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | 3.34 | 0.189 | 0.4422 | pH 7 | PMID 22775008 | Open record: β-propeller phytase (BPP) variant D56E, myo-inositol hexakisphosphate |
| β-propeller phytase (BPP)variant D56L | Bacillus amyloliquefaciens | myo-inositol hexakisphosphate | 0.1 | 0.218 | 17.67 | pH 7 | PMID 22775008 | Open record: β-propeller phytase (BPP) variant D56L, myo-inositol hexakisphosphate |