CatLogenzyme kinetics catalog

EC 3.1.4.1: phosphodiesterase I

HydrolasesOpen in Browse

CatLog read 25 records for EC 3.1.4.1 from 3 papers, for 2 organisms. Names in these papers: NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3), ENPP1 (ectonucleotide pyrophosphatase phosphodiesterase 1), Enpp1 (ectonucleotide pyrophosphatase/phosphodiesterase 1).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 55 records for EC 3.1.4.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.4.1 in Browse.

Papers

PaperOrganismsRecords
Structural basis for nucleotide recognition by the ectoenzyme CD203cHomo sapiens15
A direct, real-time, spectrophotometric assay for measuring ENPP1-catalyzed cGAMP hydrolysisHomo sapiens6
Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signalingMus musculus4

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
ENPP1 (ectonucleotide pyrophosphatase phosphodiesterase 1)Homo sapiens2′3′-cyclic GMP-AMPENPP1 assay buffer: 20 mM Tris-HCl pH 7.41.20.0065200pH 7.4, 25 °CPMID 42055331Open
ENPP1 (ectonucleotide pyrophosphatase phosphodiesterase 1)Homo sapiens2′3′-cyclic GMP-AMPENPP1 assay buffer: 20 mM Tris-HCl (pH 7.4); glycosylated1.20.0065200pH 7.4, 25 °CPMID 42055331Open
ENPP1 (ectonucleotide pyrophosphatase phosphodiesterase 1)Homo sapiens3′–5′ adenylyl-guanosine dinucleotideENPP1 assay buffer: 20 mM Tris-HCl pH 7.45.24 × 10⁻⁴1.3 × 10⁴pH 7.4, 25 °CPMID 42055331Open
ENPP1 (ectonucleotide pyrophosphatase phosphodiesterase 1)Homo sapiens3′–5′ adenylyl-guanosine dinucleotideENPP1 assay buffer: 20 mM Tris-HCl (pH 7.4); glycosylated5.24 × 10⁻⁴1.3 × 10⁴pH 7.4, 25 °CPMID 42055331Open
ENPP1 (ectonucleotide pyrophosphatase phosphodiesterase 1)Homo sapiensp-nitrophenyl 5′-thymidine monophosphateENPP1 assay buffer: 20 mM Tris-HCl pH 7.4—0.0066—pH 7.4, 25 °CPMID 42055331Open
ENPP1 (ectonucleotide pyrophosphatase phosphodiesterase 1)Homo sapiensp-nitrophenyl 5′-thymidine monophosphateENPP1 assay buffer: 20 mM Tris-HCl (pH 7.4); 10 nM ENPP1—0.0066—pH 7.4, 25 °CPMID 42055331Open
Enpp1 (ectonucleotide pyrophosphatase/phosphodiesterase 1)Mus musculusadenosine 5'-triphosphate160.046350 calc.PMID 23027977Open
Enpp1 (ectonucleotide pyrophosphatase/phosphodiesterase 1)Mus musculuscytidine 5'-triphosphate8.71.27.2 calc.PMID 23027977Open
Enpp1 (ectonucleotide pyrophosphatase/phosphodiesterase 1)Mus musculusguanosine 5'-triphosphate8204.2200 calc.PMID 23027977Open
Enpp1 (ectonucleotide pyrophosphatase/phosphodiesterase 1)Mus musculusuridine 5'-triphosphate2004.347 calc.PMID 23027977Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)Homo sapiensadenosine 5'-diphosphate1.970.074726.4 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)Homo sapiensadenosine 5'-triphosphate1.520.061524.7 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)Homo sapienscytidine 5'-triphosphate9.140.120276.0 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)Homo sapiensguanosine 5'-triphosphate3.370.123727.2 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)Homo sapiensuridine 5'-triphosphate6.360.120352.9 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant E275AHomo sapiensadenosine 5'-triphosphate0.330.03329.9 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant E275AHomo sapienscytidine 5'-triphosphate3.010.05159 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant E275AHomo sapiensguanosine 5'-triphosphate0.920.052717 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant E275AHomo sapiensuridine 5'-triphosphate0.770.070311 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant K204AHomo sapiensadenosine 5'-diphosphate0.891.1240.79 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant K204AHomo sapiensadenosine 5'-triphosphate0.550.8260.67 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant N227AHomo sapiensadenosine 5'-diphosphate2.360.070733.4 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant N227AHomo sapiensadenosine 5'-triphosphate1.460.054526.8 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant T179C/A336CHomo sapiensadenosine 5'-diphosphate2.360.080329.4 calc.pH 7.5, 37 °CPMID 29717535Open
NPP3 (CD203c, ENPP3; ecto-nucleotide pyrophosphatase/phosphodiesterase 3)variant T179C/A336CHomo sapiensadenosine 5'-triphosphate1.220.049124.8 calc.pH 7.5, 37 °CPMID 29717535Open