EC 3.2.1.227: N-acetyllactosaminidase

HydrolasesOpen in Browse

CatLog read 8 records for EC 3.2.1.227 from 1 paper, for 4 organisms. Names in these papers: AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

Papers with records for EC 3.2.1.227
Paper	Organisms	Records
Discovery of Lacto-N-Biosidases and a Novel N-Acetyllactosaminidase Activity in the CAZy Family GH20: Functional Diversity and Structural Insights	Aquimarina sp., Paludibaculum fermentans and 2 more	8

Records

Records for EC 3.2.1.227 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Aquimarina sp.	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	2.16	0.507	4.25	pH 6, 35 °C	PMID 39239753	Open record: AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Aquimarina sp.	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	3.9	0.909	4.34	pH 6, 35 °C	PMID 39239753	Open record: AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)
PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Parabacteroides distasonis	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	14.6	6.866	2.12	pH 6, 50 °C	PMID 39239753	Open record: PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Parabacteroides distasonis	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	23.6	1.769	13.4	pH 6, 50 °C	PMID 39239753	Open record: PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)
PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Paludibaculum fermentans	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	—	—	0.666	pH 6, 45 °C	PMID 39239753	Open record: PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Paludibaculum fermentans	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	17.1	1.408	13	pH 6, 45 °C	PMID 39239753	Open record: PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)
PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Planctomycetales bacterium	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	4.6	1.087	4.25	pH 6, 50 °C	PMID 39239753	Open record: PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Planctomycetales bacterium	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	8.9	1.775	4.99	pH 6, 50 °C	PMID 39239753	Open record: PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)