EC 3.2.1.41: pullulanase
HydrolasesOpen in Browse
CatLog read 15 records for EC 3.2.1.41 from 2 papers, for 2 organisms. Names in these papers: pullulanase, amylopullulanase TPApu.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 47 records for EC 3.2.1.41 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.41 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Random mutagenesis of pullulanase from Klebsiella aerogenes for studies of the structure and function of the enzyme | Klebsiella aerogenes | 8 |
| Sequence analysis and biochemical properties of an acidophilic and hyperthermophilic amylopullulanase from Thermofilum pendens | Thermofilum pendens | 7 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| amylopullulanase TPApu | Thermofilum pendens | alpha-cyclodextrin | 0.09 | 0.25 | 0.36 | pH 3.5, 90 °C | PMID 29551507 | Open record: amylopullulanase TPApu, alpha-cyclodextrin |
| amylopullulanase TPApu | Thermofilum pendens | amylopectin | 1.69 | — | — | pH 3.5, 90 °C | PMID 29551507 | Open record: amylopullulanase TPApu, amylopectin |
| amylopullulanase TPApu | Thermofilum pendens | amylose (potato) | 0.11 | — | — | pH 3.5, 90 °C | PMID 29551507 | Open record: amylopullulanase TPApu, amylose (potato) |
| amylopullulanase TPApu | Thermofilum pendens | beta-cyclodextrin | 0.11 | 5.16 | 0.02 | pH 3.5, 90 °C | PMID 29551507 | Open record: amylopullulanase TPApu, beta-cyclodextrin |
| amylopullulanase TPApu | Thermofilum pendens | gamma-cyclodextrin | 0.03 | 0.71 | 0.04 | pH 3.5, 90 °C | PMID 29551507 | Open record: amylopullulanase TPApu, gamma-cyclodextrin |
| amylopullulanase TPApu | Thermofilum pendens | pullulan | 0.73 | — | — | pH 3.5, 90 °C | PMID 29551507 | Open record: amylopullulanase TPApu, pullulan |
| amylopullulanase TPApu | Thermofilum pendens | soluble starch (potato) | 1.67 | — | — | pH 3.5, 90 °C | PMID 29551507 | Open record: amylopullulanase TPApu, soluble starch (potato) |
| pullulanase | Klebsiella aerogenes | pullulan | 33 | 0.045 | 730 | pH 6, 50 °C | PMID 7706211 | Open record: pullulanase, pullulan |
| pullulanasevariant A159E/S332R | Klebsiella aerogenes | pullulan | 35 | 0.167 | 210 | pH 6, 50 °C | PMID 7706211 | Open record: pullulanase variant A159E/S332R, pullulan |
| pullulanasevariant A515S/T617I | Klebsiella aerogenes | pullulan | 18 | 0.313 | 60 | pH 6, 50 °C | PMID 7706211 | Open record: pullulanase variant A515S/T617I, pullulan |
| pullulanasevariant F562I | Klebsiella aerogenes | pullulan | 34 | 0.031 | 1.1 × 10³ | pH 6, 50 °C | PMID 7706211 | Open record: pullulanase variant F562I, pullulan |
| pullulanasevariant L409Q/F562I | Klebsiella aerogenes | pullulan | 31 | 0.035 | 890 | pH 6, 50 °C | PMID 7706211 | Open record: pullulanase variant L409Q/F562I, pullulan |
| pullulanasevariant P403Q/L482Q | Klebsiella aerogenes | pullulan | 49 | 0.13 | 380 | pH 6, 50 °C | PMID 7706211 | Open record: pullulanase variant P403Q/L482Q, pullulan |
| pullulanasevariant Q317L/T484S | Klebsiella aerogenes | pullulan | 32 | 0.087 | 370 | pH 6, 50 °C | PMID 7706211 | Open record: pullulanase variant Q317L/T484S, pullulan |
| pullulanasevariant V441L/I600F | Klebsiella aerogenes | pullulan | 47 | 0.097 | 480 | pH 6, 50 °C | PMID 7706211 | Open record: pullulanase variant V441L/I600F, pullulan |