EC 3.2.1.74: glucan 1,4-beta-glucosidase
HydrolasesOpen in Browse
CatLog read 19 records for EC 3.2.1.74 from 2 papers, for 1 organism. Names in these papers: beta-glucosidase 1A (TnBgl1A), beta-glucan glucohydrolase (GghA).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 28 records for EC 3.2.1.74 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.74 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Crystal structure of β-glucosidase 1A from Thermotoga neapolitana and comparison of active site mutants for hydrolysis of flavonoid glucosides | Thermotoga neapolitana | 14 |
| Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana | Thermotoga neapolitana | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| beta-glucan glucohydrolase (GghA) | Thermotoga neapolitana | cellobiose | 285.0 | 28.6 | 9.96 | pH 7.2, 75 °C | PMID 10960102 | Open record: beta-glucan glucohydrolase (GghA), cellobiose |
| beta-glucan glucohydrolase (GghA) | Thermotoga neapolitana | cellotetraose | 333.7 | 2.15 | 155.2 | pH 7.2, 75 °C | PMID 10960102 | Open record: beta-glucan glucohydrolase (GghA), cellotetraose |
| beta-glucan glucohydrolase (GghA) | Thermotoga neapolitana | cellotriose | 345.7 | 4.55 | 75.98 | pH 7.2, 75 °C | PMID 10960102 | Open record: beta-glucan glucohydrolase (GghA), cellotriose |
| beta-glucan glucohydrolase (GghA) | Thermotoga neapolitana | p-nitrophenyl beta-D-glucosidelow [S] range 0 to 2 mM pNPG (row labelled Hydrolysis) | 512.5 | 0.28 | 1831 | pH 6.4, 85 °C | PMID 10960102 | Open record: beta-glucan glucohydrolase (GghA), p-nitrophenyl beta-D-glucoside |
| beta-glucan glucohydrolase (GghA) | Thermotoga neapolitana | p-nitrophenyl beta-D-glucoside | 883.9 | 1.93 | 458.0 | pH 6.4, 85 °C | PMID 10960102 | Open record: beta-glucan glucohydrolase (GghA), p-nitrophenyl beta-D-glucoside |
| beta-glucosidase 1A (TnBgl1A) | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 485 | 0.24 | 2 × 10³ | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A), 4-nitrophenyl beta-D-glucopyranoside |
| beta-glucosidase 1A (TnBgl1A) | Thermotoga neapolitana | quercetin 3-beta-D-glucoside | 13.6 | 0.13 | 101 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A), quercetin 3-beta-D-glucoside |
| beta-glucosidase 1A (TnBgl1A)variant F224I | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 803 | 0.64 | 1247 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant F224I, 4-nitrophenyl beta-D-glucopyranoside |
| beta-glucosidase 1A (TnBgl1A)variant F224I | Thermotoga neapolitana | quercetin 3-beta-D-glucoside | 14.4 | 0.15 | 95 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant F224I, quercetin 3-beta-D-glucoside |
| beta-glucosidase 1A (TnBgl1A)variant F310E | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 508 | 0.19 | 2714 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant F310E, 4-nitrophenyl beta-D-glucopyranoside |
| beta-glucosidase 1A (TnBgl1A)variant F310L | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 371 | 0.17 | 2144 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant F310L, 4-nitrophenyl beta-D-glucopyranoside |
| beta-glucosidase 1A (TnBgl1A)variant N220F | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 849 | 0.3 | 2857 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant N220F, 4-nitrophenyl beta-D-glucopyranoside |
| beta-glucosidase 1A (TnBgl1A)variant N220S | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 757 | 0.31 | 2433 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant N220S, 4-nitrophenyl beta-D-glucopyranoside |
| beta-glucosidase 1A (TnBgl1A)variant N220S | Thermotoga neapolitana | quercetin 3-beta-D-glucoside | 39.8 | 0.093 | 428 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant N220S, quercetin 3-beta-D-glucoside |
| beta-glucosidase 1A (TnBgl1A)variant N221F | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 22 | 0.24 | 93 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant N221F, 4-nitrophenyl beta-D-glucopyranoside |
| beta-glucosidase 1A (TnBgl1A)variant N221S | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 1.17 × 10³ | 0.43 | 2724 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant N221S, 4-nitrophenyl beta-D-glucopyranoside |
| beta-glucosidase 1A (TnBgl1A)variant N221S | Thermotoga neapolitana | quercetin 3-beta-D-glucoside | 40.8 | 0.092 | 446 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant N221S, quercetin 3-beta-D-glucoside |
| beta-glucosidase 1A (TnBgl1A)variant N221S/P342L | Thermotoga neapolitana | quercetin 3-beta-D-glucoside | 45.6 | 0.074 | 618 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant N221S/P342L, quercetin 3-beta-D-glucoside |
| beta-glucosidase 1A (TnBgl1A)variant W322A | Thermotoga neapolitana | 4-nitrophenyl beta-D-glucopyranoside | 61 | 0.22 | 282 | pH 5.6, 80 °C | PMID 28142197 | Open record: beta-glucosidase 1A (TnBgl1A) variant W322A, 4-nitrophenyl beta-D-glucopyranoside |