CatLogenzyme kinetics catalog

EC 3.2.2.27: uracil-DNA glycosylase

HydrolasesOpen in Browse

CatLog read 22 records for EC 3.2.2.27 from 2 papers, for 3 organisms. Names in these papers: UDGa (family 4 uracil DNA glycosylase), uracil-DNA N-glycosylase (cUNG), uracil-DNA N-glycosylase (hUNG).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 37 records for EC 3.2.2.27 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.2.27 in Browse.

Papers

PaperOrganismsRecords
Thermal unfolding studies of cold adapted uracil-DNA N-glycosylase (UNG) from Atlantic cod (Gadus morhua). A comparative study with human UNGGadus morhua, Homo sapiens12
Correlated Mutation in the Evolution of Catalysis in Uracil DNA Glycosylase SuperfamilyThermus thermophilus10

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
UDGa (family 4 uracil DNA glycosylase)Thermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)0.239.7 × 10⁻⁴240pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant E41A/G42DThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)——0.43pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant E41QThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)——0.27pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant E41Q/G42DThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)0.00751.7 × 10⁻⁴45pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant E47AThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)——2pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant F54AThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)——14pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant G42DThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)——2.7pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant H155SThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)7.2 × 10⁻⁵7.6 × 10⁻⁴0.094pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant N80AThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)——5.5pH 7.6, 60 °CPMID 28397787Open
UDGa (family 4 uracil DNA glycosylase)variant N89AThermus thermophilusdouble-stranded 69-mer oligodeoxynucleotide containing a deoxyuridine paired with guanine (G/U base pair)0.0257.4 × 10⁻⁴35pH 7.6, 60 °CPMID 28397787Open
uracil-DNA N-glycosylase (cUNG)variant G183DGadus morhuauracil (3H labeled) incorporated calf thymus DNA9.88.3 × 10⁻⁴1.188 × 10⁴pH 7.5, 37 °CPMID 21959147Open
uracil-DNA N-glycosylase (cUNG)variant G183DGadus morhuauracil (3H labeled) incorporated calf thymus DNA4.087.4 × 10⁻⁴5.48 × 10³pH 7.5, 22 °CPMID 21959147Open
uracil-DNA N-glycosylase (cUNG)variant G183DGadus morhuauracil (3H labeled) incorporated calf thymus DNA20.001131.78 × 10³pH 7.5, 15 °CPMID 21959147Open
uracil-DNA N-glycosylase (cUNG)variant G183D/R302KGadus morhuauracil (3H labeled) incorporated calf thymus DNA13.64 × 10⁻⁴3.6 × 10⁴pH 7.5, 37 °CPMID 21959147Open
uracil-DNA N-glycosylase (cUNG)variant G183D/R302KGadus morhuauracil (3H labeled) incorporated calf thymus DNA4.76 × 10⁻⁴7.82 × 10³pH 7.5, 22 °CPMID 21959147Open
uracil-DNA N-glycosylase (cUNG)variant G183D/R302KGadus morhuauracil (3H labeled) incorporated calf thymus DNA3.030.0013.15 × 10³pH 7.5, 15 °CPMID 21959147Open
uracil-DNA N-glycosylase (hUNG)variant D183GHomo sapiensuracil (3H labeled) incorporated calf thymus DNA7.730.001574.92 × 10³pH 7.5, 37 °CPMID 21959147Open
uracil-DNA N-glycosylase (hUNG)variant D183GHomo sapiensuracil (3H labeled) incorporated calf thymus DNA4.550.002771.63 × 10³pH 7.5, 22 °CPMID 21959147Open
uracil-DNA N-glycosylase (hUNG)variant D183GHomo sapiensuracil (3H labeled) incorporated calf thymus DNA1.450.00226650pH 7.5, 15 °CPMID 21959147Open
uracil-DNA N-glycosylase (hUNG)variant D183G/K302RHomo sapiensuracil (3H labeled) incorporated calf thymus DNA16.850.002656.37 × 10³pH 7.5, 37 °CPMID 21959147Open
uracil-DNA N-glycosylase (hUNG)variant D183G/K302RHomo sapiensuracil (3H labeled) incorporated calf thymus DNA4.070.002761.47 × 10³pH 7.5, 22 °CPMID 21959147Open
uracil-DNA N-glycosylase (hUNG)variant D183G/K302RHomo sapiensuracil (3H labeled) incorporated calf thymus DNA1.650.00238700pH 7.5, 15 °CPMID 21959147Open