CatLogenzyme kinetics catalog

EC 3.3.2.8: limonene-1,2-epoxide hydrolase

HydrolasesOpen in Browse

CatLog read 31 records for EC 3.3.2.8 from 2 papers, for 1 organism. Names in these papers: limonene epoxide hydrolase (LEH).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 22 records for EC 3.3.2.8 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.3.2.8 in Browse.

Papers

PaperOrganismsRecords
doi:10.1021/acscatal.6b01113Rhodococcus erythropolis19
Computational Design of Enantiocomplementary Epoxide Hydrolases for Asymmetric Synthesis of Aliphatic and Aromatic Diols—12

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
limonene epoxide hydrolase (LEH)variant L35W/L74F/I80G/I116V/F139L (32A)—cis-2,3-butene oxide0.0632252.8 × 10⁻⁴PMID 31961471Open
limonene epoxide hydrolase (LEH)variant L74I/L103V/F134Y/F139W (43A)—cyclopentene oxide0.017354.8 × 10⁻⁴PMID 31961471Open
limonene epoxide hydrolase (LEH)variant LEH-P (thermostable template)—cis-2,3-butene oxide0.056180.0031PMID 31961471Open
limonene epoxide hydrolase (LEH)variant LEH-P (thermostable template)—cis-stilbene oxide0.1470.370.406PMID 31961471Open
limonene epoxide hydrolase (LEH)variant LEH-P (thermostable template)—cyclopentene oxide0.0354.20.0079PMID 31961471Open
limonene epoxide hydrolase (LEH)variant M32A/M78I/I80F/L103I/I116V/F139L (24A)—cyclopentene oxide0.00530.0019PMID 31961471Open
limonene epoxide hydrolase (LEH)variant M32L/L103V/L114A/I116F/F139W (46C)—cyclopentene oxide0.0223446 × 10⁻⁵PMID 31961471Open
limonene epoxide hydrolase (LEH)variant M32L/L35G/I80W/L103V/F139L (60A)—cis-stilbene oxide0.0520.060.89PMID 31961471Open
limonene epoxide hydrolase (LEH)variant M32L/L35M/L103I/L114M/I116F/F139L (41B)—cis-stilbene oxide0.0020.190.0105PMID 31961471Open
limonene epoxide hydrolase (LEH)variant M32L/L35M/M78I/L103I/L114M/I116F (52A)—cis-stilbene oxide0.0030.160.019PMID 31961471Open
limonene epoxide hydrolase (LEH)variant RR8—cyclopentene oxide0.0391892 × 10⁻⁴PMID 31961471Open
limonene epoxide hydrolase (LEH)variant SS16—cyclopentene oxide0.062540.00114PMID 31961471Open
limonene epoxide hydrolase (LEH)Rhodococcus erythropoliscyclohexene oxide0.826.70.1224pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant I5C/S15P/A19K/M32V/T76K/M78V/I80V/E84C/T85V/G89C/S91C/N92K/Y96F/L114F/E124DRhodococcus erythropoliscyclohexene oxide0.5117.70.02881pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant I5C/S15P/A19K/T76K/E84C/T85V/G89C/S91C/N92K/Y96F/E124DRhodococcus erythropoliscyclohexene oxide1.318.790.149pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant I5C/S15P/A19K/T76K/I80Y/E84C/T85V/G89C/S91C/N92K/Y96F/L114V/I116V/E124DRhodococcus erythropoliscyclohexene oxide0.1919.320.00984pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant I80Y/L114V/I116VRhodococcus erythropoliscyclohexene oxide0.1119.110.00575pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant M32V/M78V/I80V/L114FRhodococcus erythropoliscyclohexene oxide0.1810.120.01778pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant S15P/A19K/E45K/T76K/T85V/N92K/Y96F/E124DRhodococcus erythropoliscyclohexene oxide1.037.740.1331pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant S15P/A19K/E45K/T76K/T85V/N92K/Y96F/E124D/I80Y/L114V/I116VRhodococcus erythropoliscyclohexene oxide0.1315.30.0085pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant S15P/A19K/M32V/E45K/T76K/M78V/I80V/T85V/N92K/Y96F/L114F/E124DRhodococcus erythropoliscyclohexene oxide0.4116.260.02521pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant S15P/M78FRhodococcus erythropoliscyclohexene oxide1.2516.970.07366pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant S15P/M78F/N92K/F139VRhodococcus erythropoliscyclohexene oxide0.8616.060.05354pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant S15P/M78F/N92K/F139V/T76K/T85KRhodococcus erythropoliscyclohexene oxide0.598.230.07169pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant S15P/M78F/N92K/F139V/T76K/T85K/E45D/I80V/E124DRhodococcus erythropoliscyclohexene oxide0.646.390.1002pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant S15P/M78F/N92K/F139V/T76K/T85K/E45D/I80V/E124DRhodococcus erythropoliscyclohexene oxide0.846.960.1207pH 7.4, 37 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant T76K/L114V/I116VRhodococcus erythropoliscyclohexene oxide1.5514.410.1076pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant T76K/L114V/I116V/N92K/F139V/L147FRhodococcus erythropoliscyclohexene oxide0.7615.440.04922pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant T76K/L114V/I116V/N92K/F139V/L147F/S15D/A19K/L74F/M78FRhodococcus erythropoliscyclohexene oxide0.8219.410.04225pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant T76K/L114V/I116V/N92K/F139V/L147F/S15D/A19K/L74F/M78F/E45DRhodococcus erythropoliscyclohexene oxide0.8416.10.05217pH 7.4, 30 °Cdoi:10.1021/acscatal.6b01113Open
limonene epoxide hydrolase (LEH)variant T76K/L114V/I116V/N92K/F139V/L147F/S15D/A19K/L74F/M78F/E45DRhodococcus erythropoliscyclohexene oxide0.9313.090.07105pH 7.4, 37 °Cdoi:10.1021/acscatal.6b01113Open