EC 3.4.11.24: aminopeptidase S
HydrolasesOpen in Browse
CatLog read 54 records for EC 3.4.11.24 from 5 papers, for 5 organisms. Names in these papers: leucine aminopeptidase (SSAP), Streptomyces griseus aminopeptidase (SgAP), Streptomyces griseus aminopeptidase (sAP), aminopeptidase (SgAP) and 3 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 13 records for EC 3.4.11.24 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.11.24 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Alteration of leucine aminopeptidase from Streptomyces septatus TH-2 to phenylalanine aminopeptidase by site-directed mutagenesis | Streptomyces septatus | 16 |
| Mechanistic role of each metal ion in Streptomyces dinuclear aminopeptidase: PEPTIDE hydrolysis and 7x10(10)-fold rate enhancement of phosphodiester hydrolysis | Streptomyces griseus | 13 |
| doi:10.1039/b004544f | Streptomyces griseus | 9 |
| A "moonlighting" dizinc aminopeptidase from Streptomyces griseus: mechanisms for peptide hydrolysis and the 4 x 10(10)-fold acceleration of the alternative phosphodiester hydrolysis | Streptomyces griseus | 8 |
| Enzymatic degradation of beta- and mixed alpha,beta-oligopeptides | Ochrobactrum anthropi, Sphingosinicella microcystinivorans and 1 more | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| 3-2W4 BapA (beta-peptidyl aminopeptidase BapA of strain 3-2W4) | Sphingosinicella xenopeptidilytica | (R)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide | — | — | 6.81 | pH 8, 25 °C | PMID 17193247 | Open record: 3-2W4 BapA (beta-peptidyl aminopeptidase BapA of strain 3-2W4), (R)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide |
| 3-2W4 BapA (beta-peptidyl aminopeptidase BapA of strain 3-2W4) | Sphingosinicella xenopeptidilytica | (S)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide | 30.2 | 1.23 | 24.6 | pH 8, 25 °C | PMID 17193247 | Open record: 3-2W4 BapA (beta-peptidyl aminopeptidase BapA of strain 3-2W4), (S)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide |
| 3-2W4 BapA (beta-peptidyl aminopeptidase BapA of strain 3-2W4) | Sphingosinicella xenopeptidilytica | (S)-3-amino-N-(4-nitrophenyl)butanamide | 13.2 | 0.7 | 18.7 | pH 8, 25 °C | PMID 17193247 | Open record: 3-2W4 BapA (beta-peptidyl aminopeptidase BapA of strain 3-2W4), (S)-3-amino-N-(4-nitrophenyl)butanamide |
| aminopeptidase (SgAP) | Streptomyces griseus | bis(4-nitrophenyl) phosphate10 mM Ca2+; 100 mM NaCl; Michaelis-Menten nonlinear fit | 0.42 | 3.4 | 0.124 | pH 8, 30 °C | PMID 17105197 | Open record: aminopeptidase (SgAP), bis(4-nitrophenyl) phosphate |
| aminopeptidase (SgAP) | Streptomyces griseus | bis(4-nitrophenyl) phosphate10 mM Ca2+ (Table 2 inhibition-study conditions) | — | 4.5 | — | pH 8, 30 °C | PMID 17105197 | Open record: aminopeptidase (SgAP), bis(4-nitrophenyl) phosphate |
| aminopeptidase (SgAP) | Streptomyces griseus | glycine p-nitroanilide | 1.1 | 1.4 | 0.76 | pH 8, 30 °C | PMID 17105197 | Open record: aminopeptidase (SgAP), glycine p-nitroanilide |
| aminopeptidase (SgAP) | Streptomyces griseus | L-alanine p-nitroanilide | 4.9 | 7.8 | 0.63 | pH 8, 30 °C | PMID 17105197 | Open record: aminopeptidase (SgAP), L-alanine p-nitroanilide |
| aminopeptidase (SgAP) | Streptomyces griseus | L-leucine p-nitroanilide | 657 | 0.45 | 1.5 × 10³ | pH 8, 30 °C | PMID 17105197 | Open record: aminopeptidase (SgAP), L-leucine p-nitroanilide |
| aminopeptidase (SgAP) | Streptomyces griseus | L-lysine p-nitroanilide | 2.8 | 5 | 0.56 | pH 8, 30 °C | PMID 17105197 | Open record: aminopeptidase (SgAP), L-lysine p-nitroanilide |
| aminopeptidase (SgAP) | Streptomyces griseus | L-methionine p-nitroanilide | 43.3 | 0.58 | 750 | pH 8, 30 °C | PMID 17105197 | Open record: aminopeptidase (SgAP), L-methionine p-nitroanilide |
| aminopeptidase (SgAP) | Streptomyces griseus | L-valine p-nitroanilide | 0.28 | 0.18 | 1.6 | pH 8, 30 °C | PMID 17105197 | Open record: aminopeptidase (SgAP), L-valine p-nitroanilide |
| L-aminopeptidase-D-amidase/esterase DmpA | Ochrobactrum anthropi | (S)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide | 0.03 | 1.1 | 0.027 | pH 8, 25 °C | PMID 17193247 | Open record: L-aminopeptidase-D-amidase/esterase DmpA, (S)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide |
| L-aminopeptidase-D-amidase/esterase DmpA | Ochrobactrum anthropi | (S)-3-amino-N-(4-nitrophenyl)butanamide | 51.4 | 0.019 | 2.6 × 10³ | pH 8, 25 °C | PMID 17193247 | Open record: L-aminopeptidase-D-amidase/esterase DmpA, (S)-3-amino-N-(4-nitrophenyl)butanamide |
| leucine aminopeptidase (SSAP) | Streptomyces septatus | L-leucinamide | 104 | 15.6 | 6.67 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP), L-leucinamide |
| leucine aminopeptidase (SSAP) | Streptomyces septatus | L-leucine methyl ester | 64.2 | 15.8 | 4.06 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP), L-leucine methyl ester |
| leucine aminopeptidase (SSAP) | Streptomyces septatus | L-leucine p-nitroanilide | 65.9 | 0.36 | 184 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP), L-leucine p-nitroanilide |
| leucine aminopeptidase (SSAP) | Streptomyces septatus | L-phenylalaninamide | 43.8 | 6.98 | 6.28 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP), L-phenylalaninamide |
| leucine aminopeptidase (SSAP) | Streptomyces septatus | L-phenylalanine methyl ester | 54.3 | 6.9 | 7.87 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP), L-phenylalanine methyl ester |
| leucine aminopeptidase (SSAP) | Streptomyces septatus | L-phenylalanine p-nitroanilide | 17.6 | 0.34 | 51.4 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP), L-phenylalanine p-nitroanilide |
| leucine aminopeptidase (SSAP)variant F221A | Streptomyces septatus | L-leucinamide | 31.7 | 42.7 | 0.74 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221A, L-leucinamide |
| leucine aminopeptidase (SSAP)variant F221A | Streptomyces septatus | L-leucine p-nitroanilide | 34 | 0.68 | 49.9 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221A, L-leucine p-nitroanilide |
| leucine aminopeptidase (SSAP)variant F221A | Streptomyces septatus | L-phenylalaninamide | 115 | 7.68 | 15 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221A, L-phenylalaninamide |
| leucine aminopeptidase (SSAP)variant F221A | Streptomyces septatus | L-phenylalanine methyl ester | 224 | 12.8 | 17.5 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221A, L-phenylalanine methyl ester |
| leucine aminopeptidase (SSAP)variant F221A | Streptomyces septatus | L-phenylalanine p-nitroanilide | 229 | 0.44 | 525 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221A, L-phenylalanine p-nitroanilide |
| leucine aminopeptidase (SSAP)variant F221I | Streptomyces septatus | L-leucinamide | 155 | 8.31 | 18.7 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221I, L-leucinamide |
| leucine aminopeptidase (SSAP)variant F221I | Streptomyces septatus | L-leucine methyl ester | 169 | 16.1 | 10.5 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221I, L-leucine methyl ester |
| leucine aminopeptidase (SSAP)variant F221I | Streptomyces septatus | L-leucine p-nitroanilide | 108 | 0.25 | 428 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221I, L-leucine p-nitroanilide |
| leucine aminopeptidase (SSAP)variant F221I | Streptomyces septatus | L-phenylalaninamide | 10.7 | 24.2 | 0.44 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221I, L-phenylalaninamide |
| leucine aminopeptidase (SSAP)variant F221I | Streptomyces septatus | L-phenylalanine p-nitroanilide | 19.3 | 1.85 | 10.4 | pH 8, 37 °C | PMID 16269763 | Open record: leucine aminopeptidase (SSAP) variant F221I, L-phenylalanine p-nitroanilide |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | bis(p-nitrophenyl)phosphateMnMn-sAP (di-Mn2+-substituted derivative) | 0.21 | 12 | 0.018 | pH 8, 30 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), bis(p-nitrophenyl)phosphate |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | bis(p-nitrophenyl)phosphateCoCo-sAP (di-Co2+-substituted derivative) | 0.74 | 9.5 | 0.078 | pH 8, 30 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), bis(p-nitrophenyl)phosphate |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | bis(p-nitrophenyl)phosphateNiNi-sAP (di-Ni2+-substituted derivative) | 0.01 | 10.6 | 9.4 × 10⁻⁴ | pH 8, 30 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), bis(p-nitrophenyl)phosphate |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | bis(p-nitrophenyl)phosphateCdCd-sAP (di-Cd2+-substituted derivative) | 0.043 | 9.7 | 0.0044 | pH 8, 30 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), bis(p-nitrophenyl)phosphate |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | p-nitrophenyl phenylphosphonateZnZn-sAP (native di-Zn2+ enzyme); excess Zn2+ present | 0.014 | 14.9 | 9.4 × 10⁻⁴ | pH 8, 50 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), p-nitrophenyl phenylphosphonate |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | p-nitrophenyl phenylphosphonateMnMn-sAP (di-Mn2+-substituted derivative) | 0.01 | 4.9 | 0.002 | pH 8, 50 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), p-nitrophenyl phenylphosphonate |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | p-nitrophenyl phenylphosphonateCoCo-sAP (di-Co2+-substituted derivative) | 0.022 | 7.9 | 0.0028 | pH 8, 50 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), p-nitrophenyl phenylphosphonate |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | p-nitrophenyl phenylphosphonateNiNi-sAP (di-Ni2+-substituted derivative) | 0.0033 | 3 | 0.0011 | pH 8, 50 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), p-nitrophenyl phenylphosphonate |
| Streptomyces griseus aminopeptidase (sAP) | Streptomyces griseus | p-nitrophenyl phenylphosphonateCdCd-sAP (di-Cd2+-substituted derivative) | 0.017 | 2.4 | 0.0071 | pH 8, 50 °C | doi:10.1039/b004544f | Open record: Streptomyces griseus aminopeptidase (sAP), p-nitrophenyl phenylphosphonate |
| Streptomyces griseus aminopeptidase (SgAP)variant CdCd-SgAP (di-Cd2+ metal derivative) | Streptomyces griseus | L-leucine p-nitroanilide | 1.68 | 0.213 | 7.89 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant CdCd-SgAP (di-Cd2+ metal derivative), L-leucine p-nitroanilide |
| Streptomyces griseus aminopeptidase (SgAP)variant CoCo-SgAP (di-Co2+ metal derivative) | Streptomyces griseus | L-leucine p-nitroanilide | 41 | 0.093 | 441 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant CoCo-SgAP (di-Co2+ metal derivative), L-leucine p-nitroanilide |
| Streptomyces griseus aminopeptidase (SgAP)variant MnCd-SgAP (Mn2+ (first site)/Cd2+ (second site) metal derivative) | Streptomyces griseus | bis(p-nitrophenyl) phosphate | 0.016 | 11 | 0.00142 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnCd-SgAP (Mn2+ (first site)/Cd2+ (second site) metal derivative), bis(p-nitrophenyl) phosphate |
| Streptomyces griseus aminopeptidase (SgAP)variant MnCd-SgAP (Mn2+ (first site)/Cd2+ (second site) metal derivative) | Streptomyces griseus | L-leucine p-nitroanilide | 1.5 | 0.19 | 7.89 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnCd-SgAP (Mn2+ (first site)/Cd2+ (second site) metal derivative), L-leucine p-nitroanilide |
| Streptomyces griseus aminopeptidase (SgAP)variant MnCo-SgAP (Mn2+ (first site)/Co2+ (second site) metal derivative) | Streptomyces griseus | bis(p-nitrophenyl) phosphate | 0.084 | 3.9 | 0.022 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnCo-SgAP (Mn2+ (first site)/Co2+ (second site) metal derivative), bis(p-nitrophenyl) phosphate |
| Streptomyces griseus aminopeptidase (SgAP)variant MnCo-SgAP (Mn2+ (first site)/Co2+ (second site) metal derivative) | Streptomyces griseus | L-leucine p-nitroanilide | 37.4 | 0.15 | 249 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnCo-SgAP (Mn2+ (first site)/Co2+ (second site) metal derivative), L-leucine p-nitroanilide |
| Streptomyces griseus aminopeptidase (SgAP)variant MnMn-SgAP (di-Mn2+ metal derivative) | Streptomyces griseus | bis(p-nitrophenyl) phosphate | 0.081 | 12.3 | 0.00467 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnMn-SgAP (di-Mn2+ metal derivative), bis(p-nitrophenyl) phosphate |
| Streptomyces griseus aminopeptidase (SgAP)variant MnMn-SgAP (di-Mn2+ metal derivative) | Streptomyces griseus | L-leucine p-nitroanilide | 27.5 | 0.88 | 31.4 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnMn-SgAP (di-Mn2+ metal derivative), L-leucine p-nitroanilide |
| Streptomyces griseus aminopeptidase (SgAP)variant MnNi-SgAP (Mn2+ (first site)/Ni2+ (second site) metal derivative) | Streptomyces griseus | bis(p-nitrophenyl) phosphate | 0.0064 | 12.8 | 5 × 10⁻⁴ | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnNi-SgAP (Mn2+ (first site)/Ni2+ (second site) metal derivative), bis(p-nitrophenyl) phosphate |
| Streptomyces griseus aminopeptidase (SgAP)variant MnNi-SgAP (Mn2+ (first site)/Ni2+ (second site) metal derivative) | Streptomyces griseus | L-leucine p-nitroanilide | 0.0505 | 0.00242 | 20.9 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnNi-SgAP (Mn2+ (first site)/Ni2+ (second site) metal derivative), L-leucine p-nitroanilide |
| Streptomyces griseus aminopeptidase (SgAP)variant MnZn-SgAP (Mn2+ (first site)/Zn2+ (second site) metal derivative) | Streptomyces griseus | bis(p-nitrophenyl) phosphate | 0.1 | 3.8 | 0.027 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnZn-SgAP (Mn2+ (first site)/Zn2+ (second site) metal derivative), bis(p-nitrophenyl) phosphate |
| Streptomyces griseus aminopeptidase (SgAP)variant MnZn-SgAP (Mn2+ (first site)/Zn2+ (second site) metal derivative) | Streptomyces griseus | L-leucine p-nitroanilide | 92.8 | 3.31 | 28 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant MnZn-SgAP (Mn2+ (first site)/Zn2+ (second site) metal derivative), L-leucine p-nitroanilide |
| Streptomyces griseus aminopeptidase (SgAP)variant NiNi-SgAP (di-Ni2+ metal derivative) | Streptomyces griseus | L-leucine p-nitroanilide | 0.0433 | 0.00229 | 18.9 | pH 8, 30 °C | PMID 19879003 | Open record: Streptomyces griseus aminopeptidase (SgAP) variant NiNi-SgAP (di-Ni2+ metal derivative), L-leucine p-nitroanilide |
| Y2 BapA (beta-peptidyl aminopeptidase BapA of strain Y2) | Sphingosinicella microcystinivorans | (R)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide | 0.22 | 1.45 | 0.154 | pH 8, 25 °C | PMID 17193247 | Open record: Y2 BapA (beta-peptidyl aminopeptidase BapA of strain Y2), (R)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide |
| Y2 BapA (beta-peptidyl aminopeptidase BapA of strain Y2) | Sphingosinicella microcystinivorans | (S)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide | — | — | 0.77 | pH 8, 25 °C | PMID 17193247 | Open record: Y2 BapA (beta-peptidyl aminopeptidase BapA of strain Y2), (S)-3-amino-N-(4-nitrophenyl)-4-phenylbutanamide |
| Y2 BapA (beta-peptidyl aminopeptidase BapA of strain Y2) | Sphingosinicella microcystinivorans | (S)-3-amino-N-(4-nitrophenyl)butanamide | 2.89 | 0.76 | 3.8 | pH 8, 25 °C | PMID 17193247 | Open record: Y2 BapA (beta-peptidyl aminopeptidase BapA of strain Y2), (S)-3-amino-N-(4-nitrophenyl)butanamide |