EC 3.4.21.50: lysyl endopeptidase
HydrolasesOpen in Browse
CatLog read 35 records for EC 3.4.21.50 from 1 paper, for 1 organism. Names in these papers: Achromobacter protease I (API, lysyl endopeptidase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 6 records for EC 3.4.21.50 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.50 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Contribution of an imidazole-indole stack to high catalytic potency of a lysine-specific serine protease, Achromobacter protease I | Achromobacter lyticus | 35 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| Achromobacter protease I (API, lysyl endopeptidase) | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 98 | 0.0028 | 3.5 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase), t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase) | Achromobacter lyticus | t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide | 46 | 0.0037 | 1.2 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase), t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase) | Achromobacter lyticus | t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide | 8.4 | 0.038 | 220 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase), t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase) | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 93 | 0.0021 | 4.4 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase), t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant H210A | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 15 | 0.0061 | 2.5 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant H210A, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant H210A | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 45 | 0.0013 | 3.5 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant H210A, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant H210F | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 51 | 0.01 | 5 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant H210F, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant H210F | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 96 | 0.0073 | 1.3 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant H210F, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant H210S | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 18 | 0.006 | 3 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant H210S, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant H210S | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 52 | 7 × 10⁻⁴ | 7.4 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant H210S, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169A | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 2.6 | 0.023 | 110 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169A, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169A | Achromobacter lyticus | t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide | 1.1 | 0.034 | 32 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169A, t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169A | Achromobacter lyticus | t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide | 0.4 | 0.066 | 6 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169A, t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169A | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 4.6 | 0.02 | 230 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169A, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169F | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 48 | 0.004 | 1.2 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169F, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169F | Achromobacter lyticus | t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide | 24 | 0.0058 | 4.1 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169F, t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169F | Achromobacter lyticus | t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide | 6 | 0.029 | 210 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169F, t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169F | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 71 | 0.0036 | 2 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169F, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169G | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 0.8 | 0.032 | 30 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169G, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169H | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 23 | 0.024 | 940 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169H, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169H | Achromobacter lyticus | t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide | 11 | 0.034 | 310 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169H, t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169H | Achromobacter lyticus | t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide | 2.7 | 0.074 | 40 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169H, t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169H | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 67 | 0.017 | 4 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169H, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169L | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 37 | 0.0046 | 8 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169L, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169L | Achromobacter lyticus | t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide | 31 | 0.0099 | 3.2 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169L, t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169L | Achromobacter lyticus | t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide | 4.5 | 0.046 | 100 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169L, t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169L | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 36 | 0.0034 | 1.1 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169L, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169V | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 20 | 0.012 | 1.7 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169V, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169V | Achromobacter lyticus | t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide | 7 | 0.042 | 170 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169V, t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169V | Achromobacter lyticus | t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide | 2.3 | 0.081 | 30 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169V, t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169V | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 28 | 0.01 | 2.8 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169V, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169Y | Achromobacter lyticus | t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide | 40 | 0.0063 | 6.3 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169Y, t-butoxycarbonyl-Ala-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169Y | Achromobacter lyticus | t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide | 22 | 0.0072 | 3.1 × 10³ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169Y, t-butoxycarbonyl-Ala-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169Y | Achromobacter lyticus | t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide | 5.2 | 0.037 | 140 | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169Y, t-butoxycarbonyl-Lys-4-methylcoumaryl-7-amide |
| Achromobacter protease I (API, lysyl endopeptidase)variant W169Y | Achromobacter lyticus | t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide | 83 | 0.0043 | 1.9 × 10⁴ | pH 9, 37 °C | PMID 11820934 | Open record: Achromobacter protease I (API, lysyl endopeptidase) variant W169Y, t-butoxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide |