EC 3.4.22.51: cruzipain
HydrolasesOpen in Browse
CatLog read 180 records for EC 3.4.22.51 from 2 papers, for 1 organism. Names in these papers: cruzain, cruzipain 2.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 14 records for EC 3.4.22.51 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.22.51 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Catalytic Mechanism of Cruzain from Trypanosoma cruzi As Determined from Solvent Kinetic Isotope Effects of Steady-State and Pre-Steady-State Kinetics | Trypanosoma cruzi | 157 |
| The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi | Trypanosoma cruzi | 23 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.89 | 0.038 | 23 | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO in H2O | 0.88 | — | 24 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO | 0.51 | — | 22 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.003 | 0.05 | 0.06 | pH 3.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.012 | 0.076 | 0.16 | pH 4.1, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.023 | 0.037 | 0.62 | pH 4.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.076 | 0.052 | 1.5 | pH 5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.21 | 0.056 | 3.8 | pH 5.6, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.44 | 0.052 | 8.2 | pH 6, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.58 | 0.035 | 17 | pH 6.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.67 | 0.029 | 23 | pH 7, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.81 | 0.032 | 25 | pH 8, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.83 | 0.032 | 25 | pH 8.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 1 | 0.058 | 17 | pH 9, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.86 | 0.036 | 24 | pH 9.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.68 | 0.031 | 22 | pH 9.9, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 3.5 | 8.5 × 10⁻⁴ | 0.016 | 0.053 calc. | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 4.0 | 0.0014 | 0.038 | 0.04 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 4.5 | 0.002 | 0.018 | 0.11 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 5.0 | 0.014 | 0.023 | 0.6 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 5.6 | 0.043 | 0.027 | 1.6 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 6.0 | 0.12 | 0.042 | 2.9 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 6.5 | 0.3 | 0.042 | 7.1 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 7.0 | 0.41 | 0.029 | 14 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 7.5 | 0.47 | 0.024 | 20 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 7.9 | 0.49 | 0.02 | 25 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 8.5 | 0.49 | 0.025 | 20 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 9.0 | 0.49 | 0.028 | 18 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 9.5 | 0.47 | 0.029 | 16 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin2% (v/v) DMSO; initial rates fitted to eq 1; assay at pD 9.9 | 0.39 | 0.022 | 18 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin50 mM MES; 1 mM EDTA; 5 mM DTT; 2% (v/v) DMSO in H2O | 7.2 | 0.0037 | 1.9 × 10³ | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO in H2O; Table 2 | 7.4 | — | 230 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 4.2 | — | 200 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin1 mM Na2EDTA; 10% (v/v) DMSO; 5 mM DTT in H2O | 15.2 | 0.007 | 2.2 × 10³ calc. | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin50 mM TAPSO; 100 mM DEA; 10% (v/v) DMSO; Table 3 | 9.5 | 0.0055 | 1.7 × 10³ calc. | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.1 | 0.078 | 2.2 | pH 3.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.4 | 0.068 | 5.9 | pH 4.1, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.7 | 0.056 | 13 | pH 4.4, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 1.9 | 0.028 | 70 | pH 5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 3.7 | 0.017 | 220 | pH 5.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 5.9 | 0.011 | 540 | pH 6, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 6.9 | 0.0066 | 1 × 10³ | pH 6.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 7.4 | 0.0046 | 1.6 × 10³ | pH 7, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 8 | 0.0043 | 1.9 × 10³ | pH 8, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 6.9 | 0.0029 | 2.4 × 10³ | pH 8.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 7.1 | 0.0036 | 2 × 10³ | pH 9, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 5.8 | 0.0041 | 1.5 × 10³ | pH 9.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 5.8 | 0.012 | 480 | pH 9.9, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | — | — | 0.23 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | — | — | 0.84 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | — | — | 2.6 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 0.3 | 0.036 | 8.3 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 1.1 | 0.022 | 50 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 2.2 | 0.016 | 140 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 3.5 | 0.013 | 270 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 4.1 | 0.0075 | 550 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 4.1 | 0.0063 | 650 | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 4 | 0.0026 | 1.5 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 3.9 | 0.0022 | 1.8 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 3.6 | 0.0021 | 1.7 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 3.5 | 0.002 | 1.8 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 2.8 | 0.0023 | 1.2 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin50 mM MES; 1 mM EDTA; 5 mM DTT; 2% (v/v) DMSO in H2O | 17 | 6.2 × 10⁻⁴ | 2.7 × 10⁴ | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO in H2O; Table 2 | 14.5 | — | — | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO in H2O; Table 2 | — | — | 2.4 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO in H2O; Table 2 | — | — | 2.6 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 7.7 | — | — | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | — | — | 1.7 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | — | — | 1.74 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin1 mM Na2EDTA; 10% (v/v) DMSO; 5 mM DTT in H2O; Table 3 | 30.5 | 0.0023 | 1.3 × 10⁴ calc. | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin50 mM TAPSO; 100 mM DEA; 10% (v/v) DMSO; Table 3 | 14.7 | 8.6 × 10⁻⁴ | 1.7 × 10⁴ calc. | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin1 mM Na2EDTA; 10% (v/v) DMSO; 5 mM DTT in H2O; Figure 7 | 34.2 | 0.0039 | 8.8 × 10³ calc. | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin50 mM TAPSO; 100 mM DEA; 10% (v/v) DMSO; Figure 7 | 14.6 | 0.0014 | 1.0 × 10⁴ calc. | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin1 mM Na2EDTA; 10% (v/v) DMSO; 5 mM DTT in H2O; Figure 7 | 34 | 0.0011 | 3.1 × 10⁴ calc. | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin50 mM TAPSO; 100 mM DEA; 10% (v/v) DMSO; Figure 7 | 14.6 | 6 × 10⁻⁴ | 2.4 × 10⁴ calc. | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 13 | 0.0052 | 2.5 × 10³ | pH 3.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 13 | 0.0019 | 6.8 × 10³ | pH 4.1, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 14 | 0.0025 | 5.6 × 10³ | pH 4.4, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 14.1 | 0.0013 | 1.1 × 10⁴ | pH 5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 13.6 | 8.7 × 10⁻⁴ | 1.6 × 10⁴ | pH 5.6, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 13.5 | 8.8 × 10⁻⁴ | 1.5 × 10⁴ | pH 6, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 13.5 | 7 × 10⁻⁴ | 1.9 × 10⁴ | pH 6.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 14 | 6.8 × 10⁻⁴ | 2.1 × 10⁴ | pH 7, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 16 | 6.1 × 10⁻⁴ | 2.6 × 10⁴ | pH 8, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 15 | 5.6 × 10⁻⁴ | 2.7 × 10⁴ | pH 8.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 14 | 5.4 × 10⁻⁴ | 2.6 × 10⁴ | pH 9, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 13 | 7.7 × 10⁻⁴ | 1.5 × 10⁴ | pH 9.5, 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 5.5 | 0.004 | 1.4 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 5.3 | 0.0029 | 1.8 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 7.6 | 0.0022 | 3.5 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 8 | 0.0017 | 4.7 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 8.4 | 0.0013 | 6.5 × 10³ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 8.3 | 8.2 × 10⁻⁴ | 1 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 8 | 5.4 × 10⁻⁴ | 1.5 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 7.6 | 4.4 × 10⁻⁴ | 1.7 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 7.5 | 4.5 × 10⁻⁴ | 1.7 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 7.2 | 4.1 × 10⁻⁴ | 1.8 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 5.8 | 3.2 × 10⁻⁴ | 1.8 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 5.2 | 3 × 10⁻⁴ | 1.7 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzain | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin5 mM DTT; 2% (v/v) DMSO; fluorimetric assay of AMC release | 4.7 | 2.9 × 10⁻⁴ | 1.6 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.017 | 0.163 | 0.1 | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.0016 | 0.149 | 0.011 | pH 4.2, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.0024 | 0.152 | 0.016 | pH 4.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.004 | 0.123 | 0.033 | pH 4.8, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.006 | 0.162 | 0.037 | pH 5.1, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.0076 | 0.155 | 0.049 | pH 5.4, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.01 | 0.166 | 0.06 | pH 5.7, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.012 | 0.162 | 0.074 | pH 6, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.014 | 0.16 | 0.088 | pH 6.3, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.014 | 0.159 | 0.088 | pH 6.6, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.013 | 0.136 | 0.096 | pH 6.9, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.016 | 0.166 | 0.096 | pH 7.2, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.0095 | 0.113 | 0.084 | pH 8, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.0099 | 0.132 | 0.075 | pH 8.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.0088 | 0.145 | 0.061 | pH 9, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.0076 | 0.146 | 0.052 | pH 9.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.0053 | 0.195 | 0.027 | pH 10, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin | 0.015 | — | 0.089 | 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-alanine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin50 mM MES; 50 mM TAPSO; 100 mM DEA; 1 mM EDTA | 0.27 | 0.045 | 6 | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.085 | 0.105 | 0.81 | pH 4.2, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.1 | 0.083 | 1.2 | pH 4.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.12 | 0.067 | 1.8 | pH 4.8, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.14 | 0.058 | 2.4 | pH 5.1, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.15 | 0.047 | 3.2 | pH 5.4, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.18 | 0.047 | 3.8 | pH 5.7, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.22 | 0.048 | 4.6 | pH 6, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.26 | 0.052 | 5 | pH 6.3, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.27 | 0.049 | 5.5 | pH 6.6, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.27 | 0.047 | 5.8 | pH 6.9, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.27 | 0.047 | 5.8 | pH 7.2, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin1 mM Na2EDTA; fluorimetric assay of AMC release | 0.27 | 0.044 | 6.2 | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.24 | 0.044 | 5.5 | pH 8, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.23 | 0.037 | 6.2 | pH 8.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.23 | 0.049 | 4.7 | pH 9, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.18 | 0.05 | 3.6 | pH 9.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.14 | 0.074 | 1.9 | pH 10, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin | 0.25 | — | 5.7 | 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-arginine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 21 | 0.0016 | 1.3 × 10⁴ | pH 7.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 18 | 0.0061 | 3 × 10³ | pH 3.6, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 19 | 0.005 | 3.8 × 10³ | pH 3.9, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 22 | 0.005 | 4.4 × 10³ | pH 4.3, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 23 | 0.0036 | 6.4 × 10³ calc. | pH 4.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 23 | 0.0028 | 8.2 × 10³ calc. | pH 4.8, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 24 | 0.0025 | 9.6 × 10³ calc. | pH 5.1, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 23 | 0.002 | 1.2 × 10⁴ calc. | pH 5.4, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 23 | 0.0017 | 1.4 × 10⁴ calc. | pH 5.7, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 23 | 0.0016 | 1.4 × 10⁴ calc. | pH 6, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 23 | 0.0015 | 1.5 × 10⁴ calc. | pH 6.3, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 22 | 0.0016 | 1.4 × 10⁴ calc. | pH 6.6, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 22 | 0.0016 | 1.4 × 10⁴ calc. | pH 6.9, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 23 | 0.0019 | 1.2 × 10⁴ calc. | pH 7.2, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 21 | 0.0013 | 1.6 × 10⁴ calc. | pH 8, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 19 | 0.0013 | 1.5 × 10⁴ calc. | pH 8.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 20 | 0.0015 | 1.3 × 10⁴ calc. | pH 9, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 19 | 0.0019 | 1.0 × 10⁴ calc. | pH 9.5, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 21 | 0.0036 | 5.8 × 10³ calc. | pH 10, 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzainvariant E208A | Trypanosoma cruzi | carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin | 21.8 | — | 1.5 × 10⁴ | 25 °C | PMID 29336553 | Open record: cruzain variant E208A, carboxybenzyl-L-phenylalanine-L-arginine-7-amino-4-methylcoumarin |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Ala-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 4.5 | 0.0044 | 1023 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Ala-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Arg-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 7.7 | 0.0032 | 2406 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Arg-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-His-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 5.6 | 0.0021 | 2667 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-His-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Leu-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 6.7 | 0.0036 | 1861 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Leu-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Ala-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 1.3 | 0.006 | 227 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Ala-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Arg-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 4.3 | 0.0073 | 589 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Arg-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Ala-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 7.7 | 0.0105 | 733 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Ala-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Arg-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 1.9 | 0.0132 | 144 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Arg-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Leu-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 9.1 | 0.0038 | 2395 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Leu-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ala-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 8.2 | 0.0051 | 1607 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ala-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Arg-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 9.8 | 0.0114 | 860 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Arg-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Phe-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 9.7 | 0.0046 | 2109 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Phe-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Pro-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 2.8 | 0.0079 | 354 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Pro-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ser-Ala-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 15.2 | 0.004 | 3.8 × 10³ | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ser-Ala-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ser-Arg-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 12.5 | 0.0046 | 2717 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ser-Arg-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 6.9 | 0.0022 | 3.09 × 10³ | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ser-Pro-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 13.9 | 0.0078 | 1782 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Arg-Phe-Ser-Pro-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Gly-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 5.6 | 0.0114 | 491 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Gly-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Lys-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 14.2 | 0.0079 | 1797 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Lys-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-Phe-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 2.5 | 0.0028 | 892 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-Phe-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Leu-S-benzyl-Cys-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 10.8 | 0.0058 | 1862 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Leu-S-benzyl-Cys-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Phe-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 1.9 | 0.0051 | 372 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Phe-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |
| cruzipain 2 | Trypanosoma cruzi | ortho-aminobenzoyl-Lys-Pro-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine | 0.5 | 0.0117 | 43 | pH 6.5, 30 °C | PMID 16734782 | Open record: cruzipain 2, ortho-aminobenzoyl-Lys-Pro-Arg-Phe-Ser-Lys-Gln-N-[2,4-dinitrophenyl]ethylenediamine |