EC 3.5.1.81: N-Acyl-D-amino-acid deacylase
HydrolasesOpen in Browse
CatLog read 15 records for EC 3.5.1.81 from 2 papers, for 2 organisms. Names in these papers: D-aminoacylase (AxD-NAase), N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 18 records for EC 3.5.1.81 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.1.81 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Engineering the substrate specificity of Alcaligenes D-aminoacylase useful for the production of D-amino acids by optical resolution | Alcaligenes xylosoxydans subsp. xylosoxydans | 9 |
| doi:10.1016/j.procbio.2012.06.003 | Variovorax paradoxus | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| D-aminoacylase (AxD-NAase) | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-alanine | 229 | 41 | 5.58 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase), N-acetyl-D-alanine |
| D-aminoacylase (AxD-NAase) | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-methionine | 1826 | 4.8 | 381 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase), N-acetyl-D-methionine |
| D-aminoacylase (AxD-NAase) | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-tryptophan | 89.5 | 6.23 | 14.4 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase), N-acetyl-D-tryptophan |
| D-aminoacylase (AxD-NAase)variant F191W | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-alanine | 330 | 38.6 | 8.56 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase) variant F191W, N-acetyl-D-alanine |
| D-aminoacylase (AxD-NAase)variant F191W | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-methionine | 829 | 2.03 | 407 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase) variant F191W, N-acetyl-D-methionine |
| D-aminoacylase (AxD-NAase)variant F191W | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-tryptophan | 669 | 2.97 | 225 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase) variant F191W, N-acetyl-D-tryptophan |
| D-aminoacylase (AxD-NAase)variant L298A | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-alanine | 5.97 | 330 | 0.02 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase) variant L298A, N-acetyl-D-alanine |
| D-aminoacylase (AxD-NAase)variant L298A | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-methionine | 463 | 56.4 | 8.21 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase) variant L298A, N-acetyl-D-methionine |
| D-aminoacylase (AxD-NAase)variant L298A | Alcaligenes xylosoxydans subsp. xylosoxydans | N-acetyl-D-tryptophan | 404 | 6.36 | 63.5 | pH 7, 30 °C | PMID 21546325 | Open record: D-aminoacylase (AxD-NAase) variant L298A, N-acetyl-D-tryptophan |
| N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase) | Variovorax paradoxus | N-acetyl-D-methionine | 57.53 | 27.89 | 2.06 | 4 °C | doi:10.1016/j.procbio.2012.06.003 | Open record: N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase), N-acetyl-D-methionine |
| N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase)variant M221L | Variovorax paradoxus | N-acetyl-D-methionine | 76.08 | 15.52 | 4.9 | 4 °C | doi:10.1016/j.procbio.2012.06.003 | Open record: N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase) variant M221L, N-acetyl-D-methionine |
| N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase)variant M254L | Variovorax paradoxus | N-acetyl-D-methionine | 54.06 | 13.73 | 3.94 | 4 °C | doi:10.1016/j.procbio.2012.06.003 | Open record: N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase) variant M254L, N-acetyl-D-methionine |
| N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase)variant M352L | Variovorax paradoxus | N-acetyl-D-methionine | 34.56 | 14.36 | 2.41 | 4 °C | doi:10.1016/j.procbio.2012.06.003 | Open record: N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase) variant M352L, N-acetyl-D-methionine |
| N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase)variant M39L | Variovorax paradoxus | N-acetyl-D-methionine | 61.31 | 19.64 | 3.12 | 4 °C | doi:10.1016/j.procbio.2012.06.003 | Open record: N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase) variant M39L, N-acetyl-D-methionine |
| N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase)variant M56L | Variovorax paradoxus | N-acetyl-D-methionine | 25.2 | 26.3 | 0.958 | 4 °C | doi:10.1016/j.procbio.2012.06.003 | Open record: N-acyl-D-amino acid amidohydrolase (N-D-AAase, D-aminoacylase) variant M56L, N-acetyl-D-methionine |