EC 3.5.1.86: mandelamide amidase
HydrolasesOpen in Browse
CatLog read 12 records for EC 3.5.1.86 from 1 paper, for 1 organism. Names in these papers: mandelamide hydrolase (MAH).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 12 records for EC 3.5.1.86 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.1.86 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Specificity and mechanism of mandelamide hydrolase catalysis | Pseudomonas putida | 12 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| mandelamide hydrolase (MAH) | Pseudomonas putida | (S)-2-hydroxy-2-phenylacetamide | 150 | 0.018 | 8.5 × 10³ | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH), (S)-2-hydroxy-2-phenylacetamide |
| mandelamide hydrolase (MAH) | Pseudomonas putida | 2-(4-nitrophenyl)acetamide | 43 | 0.036 | 1.2 × 10³ | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH), 2-(4-nitrophenyl)acetamide |
| mandelamide hydrolase (MAH) | Pseudomonas putida | 4-nitrophenyl acetate | 5.1 | 2.3 | 2.2 | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH), 4-nitrophenyl acetate |
| mandelamide hydrolase (MAH) | Pseudomonas putida | 4-nitrophenyl butanoate | — | — | 0.012 | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH), 4-nitrophenyl butanoate |
| mandelamide hydrolase (MAH) | Pseudomonas putida | ethyl 4-nitrophenyl carbonate | — | — | 0.017 | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH), ethyl 4-nitrophenyl carbonate |
| mandelamide hydrolase (MAH) | Pseudomonas putida | methyl 2-(4-nitrophenyl)acetate | 44 | 0.4 | 110 | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH), methyl 2-(4-nitrophenyl)acetate |
| mandelamide hydrolase (MAH) | Pseudomonas putida | methyl 4-nitrophenyl carbonate | 0.52 | 0.47 | 1.1 | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH), methyl 4-nitrophenyl carbonate |
| mandelamide hydrolase (MAH) | Pseudomonas putida | phenyl acetate | — | — | 0.49 | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH), phenyl acetate |
| mandelamide hydrolase (MAH)variant F150W | Pseudomonas putida | (S)-2-hydroxy-2-phenylacetamide | 43 | 0.009 | 4.6 × 10³ | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH) variant F150W, (S)-2-hydroxy-2-phenylacetamide |
| mandelamide hydrolase (MAH)variant F433A | Pseudomonas putida | (S)-2-hydroxy-2-phenylacetamide | 20 | 1.53 | 13 | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH) variant F433A, (S)-2-hydroxy-2-phenylacetamide |
| mandelamide hydrolase (MAH)variant F433L | Pseudomonas putida | (S)-2-hydroxy-2-phenylacetamide | 105 | 0.135 | 780 | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH) variant F433L, (S)-2-hydroxy-2-phenylacetamide |
| mandelamide hydrolase (MAH)variant F433W | Pseudomonas putida | (S)-2-hydroxy-2-phenylacetamide | 90 | 0.043 | 2.3 × 10³ | pH 7.8, 25 °C | PMID 28129982 | Open record: mandelamide hydrolase (MAH) variant F433W, (S)-2-hydroxy-2-phenylacetamide |