EC 4.1.2.10: (R)-mandelonitrile lyase
LyasesOpen in Browse
CatLog read 45 records for EC 4.1.2.10 from 5 papers, for 5 organisms. Names in these papers: R-hydroxynitrile lyase (ChuaHNL), hydroxynitrile lyase (ChuaHNL), (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), PeHNL-Ny and 5 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 39 records for EC 4.1.2.10 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.2.10 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| R-hydroxynitrile lyase from the cyanogenic millipede, Chamberlinius hualienensis-A new entry to the carrier protein family Lipocalines | Chamberlinius hualienensis | 10 |
| Purification and characterization of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (Loquat) | Eriobotrya japonica | 9 |
| Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis | Chamberlinius hualienensis | 9 |
| Stabilization of Hydroxynitrile Lyases from Two Variants of Passion Fruit, Passiflora edulis Sims and Passiflora edulis Forma flavicarpa, by C-Terminal Truncation | Passiflora edulis Sims, Passiflora edulis forma flavicarpa | 9 |
| doi:10.1002/adsc.201601332 | Prunus communis | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | (R,S)-2-hydroxy-2-phenylacetonitrile (racemic mandelonitrile) | 56 | 0.161 | 348 | pH 5.5 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), (R,S)-2-hydroxy-2-phenylacetonitrile (racemic mandelonitrile) |
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | 1,3-benzodioxole-5-carbaldehyde | 334 | 2.6 | 29.2 | pH 4 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), 1,3-benzodioxole-5-carbaldehyde |
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | 2,2-dimethylpropanal | 164 | 39.3 | 14.3 | pH 4 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), 2,2-dimethylpropanal |
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | 2-methylpropanal | 110 | 41.8 | 9.6 | pH 4 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), 2-methylpropanal |
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | 4-methoxybenzaldehyde | 582 | 3.5 | 50.8 | pH 4 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), 4-methoxybenzaldehyde |
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | Benzaldehyde | 1.7 × 10³ | 11.5 | 148 | pH 4 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), Benzaldehyde |
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | naphthalene-2-carbaldehyde | 1.16 × 10³ | 1.1 | 101 | pH 4 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), naphthalene-2-carbaldehyde |
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | propanal | 223 | 99.9 | 19.4 | pH 4 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), propanal |
| (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase) | Eriobotrya japonica | thiophene-2-carbaldehyde | 1.84 × 10³ | 35.7 | 161 | pH 4 | PMID 18540101 | Open record: (R)-hydroxynitrile lyase (EjHNL, mandelonitrile lyase), thiophene-2-carbaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | 2,4-dimethylbenzaldehyde | 300 | 1.3 | 230 | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), 2,4-dimethylbenzaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | 3-methoxybenzaldehyde | 1.68 × 10³ | 7.7 | 220 | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), 3-methoxybenzaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | 3-methylbenzaldehyde | 6.89 × 10³ | 10.5 | 660 | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), 3-methylbenzaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | 4-bromobenzaldehyde | 8.61 × 10³ | 10 | 860 | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), 4-bromobenzaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | 4-methoxybenzaldehyde | 3.48 × 10³ | 7.5 | 460 | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), 4-methoxybenzaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | 4-methylbenzaldehyde | 5.52 × 10³ | 5.4 | 1 × 10³ | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), 4-methylbenzaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | benzaldehyde | 3.39 × 10³ | 3 | 1.1 × 10³ | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), benzaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | biphenyl-4-carbaldehyde | 49 | 10.3 | 4.7 | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), biphenyl-4-carbaldehyde |
| hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | thiophene-2-carbaldehyde | 1.49 × 10³ | 18 | 83 | 22 °C | PMID 26261304 | Open record: hydroxynitrile lyase (ChuaHNL), thiophene-2-carbaldehyde |
| hydroxynitrile lyase isozyme PcHNL1 | Prunus communis | 2-hydroxy-2-phenylacetonitrile | 240 | 1.16 | 207 | pH 5 | doi:10.1002/adsc.201601332 | Open record: hydroxynitrile lyase isozyme PcHNL1, 2-hydroxy-2-phenylacetonitrile |
| hydroxynitrile lyase isozyme PcHNL1 | Prunus communis | 4-(methylsulfanyl)benzaldehyde | 139 | 2.29 | 61 | pH 4.2, 25 °C | doi:10.1002/adsc.201601332 | Open record: hydroxynitrile lyase isozyme PcHNL1, 4-(methylsulfanyl)benzaldehyde |
| hydroxynitrile lyase isozyme PcHNL2 | Prunus communis | 2-hydroxy-2-phenylacetonitrile | 380 | 1.47 | 258 | pH 5 | doi:10.1002/adsc.201601332 | Open record: hydroxynitrile lyase isozyme PcHNL2, 2-hydroxy-2-phenylacetonitrile |
| hydroxynitrile lyase isozyme PcHNL2 | Prunus communis | 4-(methylsulfanyl)benzaldehyde | 40.3 | 1.09 | 37 | pH 4.2, 25 °C | doi:10.1002/adsc.201601332 | Open record: hydroxynitrile lyase isozyme PcHNL2, 4-(methylsulfanyl)benzaldehyde |
| hydroxynitrile lyase isozyme PcHNL4 | Prunus communis | 2-hydroxy-2-phenylacetonitrile | 818 | 1.91 | 428 | pH 5 | doi:10.1002/adsc.201601332 | Open record: hydroxynitrile lyase isozyme PcHNL4, 2-hydroxy-2-phenylacetonitrile |
| hydroxynitrile lyase isozyme PcHNL4 | Prunus communis | 4-(methylsulfanyl)benzaldehyde | 26.3 | 1.54 | 17 | pH 4.2, 25 °C | doi:10.1002/adsc.201601332 | Open record: hydroxynitrile lyase isozyme PcHNL4, 4-(methylsulfanyl)benzaldehyde |
| hydroxynitrile lyase isozyme PcHNL5 | Prunus communis | 2-hydroxy-2-phenylacetonitrile | 817 | 6.36 | 128 | pH 5 | doi:10.1002/adsc.201601332 | Open record: hydroxynitrile lyase isozyme PcHNL5, 2-hydroxy-2-phenylacetonitrile |
| hydroxynitrile lyase isozyme PcHNL5 | Prunus communis | 4-(methylsulfanyl)benzaldehyde | 142 | 2.97 | 48 | pH 4.2, 25 °C | doi:10.1002/adsc.201601332 | Open record: hydroxynitrile lyase isozyme PcHNL5, 4-(methylsulfanyl)benzaldehyde |
| PeHNL-Np | Passiflora edulis Sims | benzaldehyde | 35.7 | 19.2 | 1.86 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Np, benzaldehyde |
| PeHNL-Np | Passiflora edulis Sims | benzaldehydeexpressed in E. coli; nonglycosylated | 33.6 | 25.2 | 1.33 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Np, benzaldehyde |
| PeHNL-Ny | Passiflora edulis forma flavicarpa | benzaldehyde | 50.4 | 13.7 | 3.7 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Ny, benzaldehyde |
| PeHNL-Ny | Passiflora edulis forma flavicarpa | benzaldehydeexpressed in E. coli from pColdI-PeHNL; nonglycosylated | 36.4 | 26.3 | 1.4 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Ny, benzaldehyde |
| PeHNL-Nyvariant PeHNLΔ105 | Passiflora edulis forma flavicarpa | benzaldehyde | 24.1 | 33 | 0.73 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Ny variant PeHNLΔ105, benzaldehyde |
| PeHNL-Nyvariant PeHNLΔ107 | Passiflora edulis forma flavicarpa | benzaldehyde | 37.2 | 26.6 | 1.4 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Ny variant PeHNLΔ107, benzaldehyde |
| PeHNL-Nyvariant PeHNLΔ109 | Passiflora edulis forma flavicarpa | benzaldehyde | 39 | 26.1 | 1.5 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Ny variant PeHNLΔ109, benzaldehyde |
| PeHNL-Nyvariant PeHNLΔ112 | Passiflora edulis forma flavicarpa | benzaldehyde | 63.1 | 25 | 2.53 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Ny variant PeHNLΔ112, benzaldehyde |
| PeHNL-Nyvariant PeHNLΔ117 | Passiflora edulis forma flavicarpa | benzaldehyde | 53.4 | 23.4 | 2.3 | pH 4, 25 °C | PMID 31562666 | Open record: PeHNL-Ny variant PeHNLΔ117, benzaldehyde |
| R-hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | (R)-mandelonitrile | 142 | 8.7 | 16.4 | pH 5 | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL), (R)-mandelonitrile |
| R-hydroxynitrile lyase (ChuaHNL) | Chamberlinius hualienensis | benzaldehyde | 2.11 × 10³ | 4.9 | 430 | pH 4.2, 22 °C | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL), benzaldehyde |
| R-hydroxynitrile lyase (ChuaHNL)variant D56E | Chamberlinius hualienensis | (R)-mandelonitrile | 6 | 1.3 | 4.8 | pH 5 | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL) variant D56E, (R)-mandelonitrile |
| R-hydroxynitrile lyase (ChuaHNL)variant D56E | Chamberlinius hualienensis | benzaldehyde | 102 | 7.1 | 14.6 | pH 4.2, 22 °C | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL) variant D56E, benzaldehyde |
| R-hydroxynitrile lyase (ChuaHNL)variant K117R | Chamberlinius hualienensis | (R)-mandelonitrile | 0.44 | 2.93 | 0.15 | pH 5 | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL) variant K117R, (R)-mandelonitrile |
| R-hydroxynitrile lyase (ChuaHNL)variant K117R | Chamberlinius hualienensis | benzaldehyde | 141 | 40 | 3.6 | pH 4.2, 22 °C | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL) variant K117R, benzaldehyde |
| R-hydroxynitrile lyase (ChuaHNL)variant Y103F | Chamberlinius hualienensis | (R)-mandelonitrile | 1.4 | 1 | 1.4 | pH 5 | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL) variant Y103F, (R)-mandelonitrile |
| R-hydroxynitrile lyase (ChuaHNL)variant Y103F | Chamberlinius hualienensis | benzaldehyde | 14 | 4.9 | 3 | pH 4.2, 22 °C | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL) variant Y103F, benzaldehyde |
| R-hydroxynitrile lyase (ChuaHNL)variant Y40F | Chamberlinius hualienensis | (R)-mandelonitrile | 18 | 0.9 | 20.4 | pH 5 | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL) variant Y40F, (R)-mandelonitrile |
| R-hydroxynitrile lyase (ChuaHNL)variant Y40F | Chamberlinius hualienensis | benzaldehyde | 66 | 1.6 | 40 | pH 4.2, 22 °C | PMID 32679618 | Open record: R-hydroxynitrile lyase (ChuaHNL) variant Y40F, benzaldehyde |