CatLogenzyme kinetics catalog

EC 4.1.99.5: aldehyde oxygenase (deformylating)

LyasesOpen in Browse

CatLog read 57 records for EC 4.1.99.5 from 2 papers, for 2 organisms. Names in these papers: cADO-1593 (aldehyde-deformylating oxygenase, ADO), aldehyde-deformylating oxygenase cADO-1593, aldehyde-deformylating oxygenase cADO-sll0208.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 10 records for EC 4.1.99.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.99.5 in Browse.

Papers

PaperOrganismsRecords
Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain lengthSynechococcus elongatus29
Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationshipSynechococcus elongatus, Synechocystis sp.28

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
aldehyde-deformylating oxygenase cADO-1593Synechococcus elongatusn-heptanal0.0075——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant D143ASynechococcus elongatusn-heptanal0.006——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant F150YSynechococcus elongatusn-heptanal5 µM cADO0.0127——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant F150YSynechococcus elongatusn-heptanalKm and Vmax from Michaelis-Menten fit (GraphPad Prism 5)0.01250.20.0625pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant L146TSynechococcus elongatusn-heptanal5 µM cADO0.0145——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant L146TSynechococcus elongatusn-heptanalKm and Vmax from Michaelis-Menten fit (GraphPad Prism 5)0.01570.340.046pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant N123HSynechococcus elongatusn-heptanal0.0072——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant N149ASynechococcus elongatusn-heptanal0.0052——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant Q110LSynechococcus elongatusn-heptanal0.0038——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant Q49H/F150YSynechococcus elongatusn-heptanal5 µM cADO0.012——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant Q49H/F150YSynechococcus elongatusn-heptanalKm and Vmax from Michaelis-Menten fit (GraphPad Prism 5)0.01450.330.044pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant Q49H/N123H/F150YSynechococcus elongatusn-heptanal5 µM cADO0.0145——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant Q49H/N123H/F150YSynechococcus elongatusn-heptanalKm and Vmax from Michaelis-Menten fit (GraphPad Prism 5)0.01730.350.0495pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant R62ASynechococcus elongatusn-heptanal6.7 × 10⁻⁴——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant W178RSynechococcus elongatusn-heptanal5 µM cADO0.0245——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant W178RSynechococcus elongatusn-heptanalKm and Vmax from Michaelis-Menten fit (GraphPad Prism 5)0.03020.340.0887pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant Y122FSynechococcus elongatusn-heptanal0.0025——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-1593variant Y39FSynechococcus elongatusn-heptanal0.0062——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208Synechocystis sp.n-heptanal5 µM cADO0.0073——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208Synechocystis sp.n-heptanalKm and Vmax from Michaelis-Menten fit (GraphPad Prism 5)0.00980.350.0282pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208variant D49HSynechocystis sp.n-heptanal0.0037——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208variant D49H/N123HSynechocystis sp.n-heptanal5 µM cADO0.0122——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208variant D49H/N123HSynechocystis sp.n-heptanalKm and Vmax from Michaelis-Menten fit (GraphPad Prism 5)0.01380.380.0363pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208variant L148RSynechocystis sp.n-heptanal5 µM cADO0.0125——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208variant L148RSynechocystis sp.n-heptanalKm and Vmax from Michaelis-Menten fit (GraphPad Prism 5)0.01520.320.0473pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208variant N123HSynechocystis sp.n-heptanal0.0023——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208variant T146LSynechocystis sp.n-heptanal0.0028——pH 7.2PMID 28302170Open
aldehyde-deformylating oxygenase cADO-sll0208variant Y150FSynechocystis sp.n-heptanal0.007——pH 7.2PMID 28302170Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-heptanal2 mM aldehyde; Table 10.01320.590.022pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-heptanal2 mM C_{7,8,9} aldehyde substrate; no competition substrate0.0133——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-heptanal2 mM C_{7,8,9} aldehyde substrate; apparent kcat; Table 20.0133——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-heptanal2 mM C_{7,8,9} aldehyde substrate; apparent kcat; Table 20.0128——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-hexanal0.030.930.032pH 7.2PMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-nonanal2 mM aldehyde; Table 10.01020.0690.15pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-nonanal2 mM C_{7,8,9} aldehyde substrate; no competition substrate0.004——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-nonanal2 mM C_{7,8,9} aldehyde substrate; apparent kcat; Table 20.0038——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-nonanal2 mM C_{7,8,9} aldehyde substrate; apparent kcat; Table 20.003——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-octanal2 mM aldehyde; Table 10.0230.180.13pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-octanal2 mM C_{7,8,9} aldehyde substrate; no competition substrate0.0055——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-octanal2 mM C_{7,8,9} aldehyde substrate; apparent kcat; Table 20.0058——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)Synechococcus elongatusn-octanal2 mM C_{7,8,9} aldehyde substrate; apparent kcat; Table 20.004——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant A121FSynechococcus elongatusn-heptanal2 mM aldehyde; Table 10.0250.560.045pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant A121FSynechococcus elongatusn-heptanal2 mM C_{7,8,9} aldehyde substrate; no competition substrate0.0235——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant A121FSynechococcus elongatusn-heptanal2 mM C_{7,8,9} aldehyde substrate; apparent kcat; Table 20.0125——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant A121FSynechococcus elongatusn-heptanal2 mM C_{7,8,9} aldehyde substrate; apparent kcat; Table 20.0115——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant A121FSynechococcus elongatusn-hexanal0.1150.960.12pH 7.2PMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant A121FSynechococcus elongatusn-nonanal0.01280.140.09pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant A121FSynechococcus elongatusn-octanal0.01630.180.09pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant I24YSynechococcus elongatusn-heptanalno competition substrate0.0175——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant I24YSynechococcus elongatusn-heptanal0.0137——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant I24YSynechococcus elongatusn-heptanal0.0065——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant L198FSynechococcus elongatusn-nonanalno competition substrate0.0083——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant L198FSynechococcus elongatusn-nonanallike another record0.0042——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant L198FSynechococcus elongatusn-nonanallike another record0.0042——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant M193YSynechococcus elongatusn-octanalno competition substrate0.0175——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant M193YSynechococcus elongatusn-octanal0.0175——pH 7.2, 37 °CPMID 27588038Open
cADO-1593 (aldehyde-deformylating oxygenase, ADO)variant M193YSynechococcus elongatusn-octanal0.0087——pH 7.2, 37 °CPMID 27588038Open