EC 4.2.99.20: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
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CatLog read 11 records for EC 4.2.99.20 from 2 papers, for 1 organism. Names in these papers: (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH), SHCHC synthase (MenH).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 13 records for EC 4.2.99.20 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.99.20 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Molecular basis of the general base catalysis of an α/β-hydrolase catalytic triad | Escherichia coli | 6 |
| Identification and characterization of (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase in the menaquinone biosynthesis of Escherichia coli | Escherichia coli | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH) | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 8.5 | 0.013 | 530 | pH 7 | PMID 24737327 | Open record: (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH), (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH)variant F153A | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 3.3 | 0.07 | 48 | pH 7 | PMID 24737327 | Open record: (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH) variant F153A, (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH)variant V152A | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 0.128 | 0.3 | 0.43 | pH 7 | PMID 24737327 | Open record: (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH) variant V152A, (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH)variant V152G | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 0.2 | 0.16 | 1.22 | pH 7 | PMID 24737327 | Open record: (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH) variant V152G, (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH)variant Y148A | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 0.125 | 0.05 | 2.7 | pH 7 | PMID 24737327 | Open record: (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH) variant Y148A, (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH)variant Y148F | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 0.58 | 0.036 | 16.3 | pH 7 | PMID 24737327 | Open record: (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (MenH) variant Y148F, (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| SHCHC synthase (MenH) | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 167 | 0.0083 | 2 × 10⁴ | pH 7, 37 °C | PMID 18284213 | Open record: SHCHC synthase (MenH), (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| SHCHC synthase (MenH) | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 147 | 0.0101 | 1.4 × 10⁴ | pH 7, 25 °C | PMID 18284213 | Open record: SHCHC synthase (MenH), (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| SHCHC synthase (MenH)variant D210A | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 0.18 | 0.118 | 1.5 | pH 7, 25 °C | PMID 18284213 | Open record: SHCHC synthase (MenH) variant D210A, (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| SHCHC synthase (MenH)variant H232A | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 5.8 × 10⁻⁴ | 0.0086 | 0.067 | pH 7, 25 °C | PMID 18284213 | Open record: SHCHC synthase (MenH) variant H232A, (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
| SHCHC synthase (MenH)variant S86A | Escherichia coli | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | 0.29 | 0.028 | 10 | pH 7, 25 °C | PMID 18284213 | Open record: SHCHC synthase (MenH) variant S86A, (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |