CatLogenzyme kinetics catalog

EC 6.1.1.11: serine-tRNA ligase

LigasesOpen in Browse

CatLog read 22 records for EC 6.1.1.11 from 1 paper, for 1 organism. Names in these papers: seryl-tRNA synthetase (mMbSerRS).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 75 records for EC 6.1.1.11 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.1.1.11 in Browse.

Papers

PaperOrganismsRecords
Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognitionMethanosarcina barkeri22

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
seryl-tRNA synthetase (mMbSerRS)Methanosarcina barkeriEscherichia coli tRNASer in total E. coli tRNA0.01750.002347.479pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)Methanosarcina barkeriL-serineATP-PPi exchange assay at 37 °C: 100 mM Hepes pH 7.6; ATP2.060.00661311.4pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)Methanosarcina barkeriL-serineaminoacylation assay at 37 °C: 50 mM Hepes/KOH pH 7.60.4730.0111342.47pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)Methanosarcina barkeriMethanococcus jannaschii tRNASec (UCA) in vitro transcript0.0050.002412.075pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)Methanosarcina barkeriMethanococcus maripaludis tRNASer (GCU) in vitro transcript0.0790.00331623.82pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)Methanosarcina barkeriMethanosarcina barkeri tRNASer (CGA isoacceptor) expressed in vivo in E. coli0.4670.00307152.1pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)Methanosarcina barkeriMethanosarcina barkeri tRNASer (CGA isoacceptor) in vitro transcript0.4390.010143.47pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)Methanosarcina barkeriMethanosarcina barkeri tRNASer (GGA isoacceptor) expressed in vivo in E. coli0.4540.00299152.1pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant E338AMethanosarcina barkeriMethanosarcina barkeri tRNASer (GGA isoacceptor) expressed in vivo in E. coli0.0590.0021727.22pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant G340V/G341AMethanosarcina barkeriMethanosarcina barkeri tRNASer (GGA isoacceptor) expressed in vivo in E. coli0.00110.006760.171pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant N435AMethanosarcina barkeriL-serineATP-PPi exchange assay at 37 °C: 100 mM Hepes pH 7.6; ATP0.1720.070282.45pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant N435AMethanosarcina barkeriL-serineaminoacylation assay at 37 °C: 50 mM Hepes/KOH pH 7.60.4350.28311.54pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant N435A/S437AMethanosarcina barkeriL-serineATP-PPi exchange assay at 37 °C: 100 mM Hepes pH 7.6; ATP0.5760.68950.84pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant N435A/S437AMethanosarcina barkeriL-serineaminoacylation assay at 37 °C: 50 mM Hepes/KOH pH 7.60.3610.50990.71pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant N435A/S437A/W396AMethanosarcina barkeriL-serineATP-PPi exchange assay at 37 °C: 100 mM Hepes pH 7.6; ATP1.322.8790.46pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant N435A/S437A/W396AMethanosarcina barkeriL-serineaminoacylation assay at 37 °C: 50 mM Hepes/KOH pH 7.60.1751.1910.15pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant R267AMethanosarcina barkeriMethanosarcina barkeri tRNASer (GGA isoacceptor) expressed in vivo in E. coli0.0320.00349.436pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant R347AMethanosarcina barkeriMethanosarcina barkeri tRNASer (GGA isoacceptor) expressed in vivo in E. coli0.00260.006380.403pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant S437AMethanosarcina barkeriL-serineATP-PPi exchange assay at 37 °C: 100 mM Hepes pH 7.6; ATP0.7290.57381.27pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant S437AMethanosarcina barkeriL-serineaminoacylation assay at 37 °C: 50 mM Hepes/KOH pH 7.60.3960.41290.96pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant W396AMethanosarcina barkeriL-serineATP-PPi exchange assay at 37 °C: 100 mM Hepes pH 7.6; ATP0.2870.26451.08pH 7.6, 37 °CPMID 18422966Open
seryl-tRNA synthetase (mMbSerRS)variant W396AMethanosarcina barkeriL-serineaminoacylation assay at 37 °C: 50 mM Hepes/KOH pH 7.60.2390.76020.32pH 7.6, 37 °CPMID 18422966Open