CatLogenzyme kinetics catalog

EC 6.2.1.71: 2,3-dihydroxybenzoate-[aryl-carrier protein] ligase

LigasesOpen in Browse

CatLog read 22 records for EC 6.2.1.71 from 2 papers, for 1 organism. Names in these papers: EntE, dihydroxybenzoate-AMP ligase (EntE).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 14 records for EC 6.2.1.71 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.2.1.71 in Browse.

Papers

PaperOrganismsRecords
Precise Probing of Residue Roles by NRPS Code Swapping: Mutation, Enzymatic Characterization, Modeling, and Substrate Promiscuity of Aryl Acid Adenylation DomainsEscherichia coli15
Kinetic and inhibition studies of dihydroxybenzoate-AMP ligase from Escherichia coliEscherichia coli7

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
dihydroxybenzoate-AMP ligase (EntE)Escherichia coli2,3-dihydroxybenzoic acid2.80.0025880pH 7.8, 25 °CPMID 20359185Open
dihydroxybenzoate-AMP ligase (EntE)Escherichia coli2-hydroxybenzoic acid0.80.0711pH 7.8, 25 °CPMID 20359185Open
dihydroxybenzoate-AMP ligase (EntE)Escherichia coli3-hydroxybenzoic acid0.30.074.6pH 7.8, 25 °CPMID 20359185Open
dihydroxybenzoate-AMP ligase (EntE)Escherichia coli4-amino-2-hydroxybenzoic acid4.43.115pH 7.8, 25 °CPMID 20359185Open
dihydroxybenzoate-AMP ligase (EntE)Escherichia coliadenosine 5'-triphosphate2.80.43390pH 7.8, 25 °CPMID 20359185Open
dihydroxybenzoate-AMP ligase (EntE)Escherichia coliD-pantetheine0.934.20.026pH 7.8, 25 °CPMID 20359185Open
dihydroxybenzoate-AMP ligase (EntE)Escherichia coliphosphopantetheinylated EntB aryl carrier protein domain (EntB residues 188-285, N-terminal His6 tag)2.80.0029980pH 7.8, 25 °CPMID 20359185Open
EntEvariant N340CEscherichia coli2,3-dihydroxybenzoic acid0.01620.00276.08PMID 31894971Open
EntEvariant N340CEscherichia colisalicylic acid0.130.008914.65PMID 31894971Open
EntEvariant V339IEscherichia coli2,3-dihydroxybenzoic acid0.030.00614.97PMID 31894971Open
EntEvariant V339IEscherichia colisalicylic acid0.1480.005526.93PMID 31894971Open
EntEvariant V339LEscherichia coli2,3-dihydroxybenzoic acid0.0250.00495.3PMID 31894971Open
EntEvariant V339LEscherichia colisalicylic acid0.1550.00819.5PMID 31894971Open
EntEvariant Y236FEscherichia coli2,3-dihydroxybenzoic acid0.0270.00713.63PMID 31894971Open
EntEvariant Y236FEscherichia colisalicylic acid0.1150.006118.98PMID 31894971Open
EntEvariant Y236F/S240CEscherichia coli2,3-dihydroxybenzoic acid0.0820.4080.4PMID 31894971Open
EntEvariant Y236F/S240CEscherichia colisalicylic acid0.1380.0393.6PMID 31894971Open
EntEvariant Y236F/S240C/N340CEscherichia coli2,3-dihydroxybenzoic acid0.1330.3180.42PMID 31894971Open
EntEvariant Y236F/S240C/N340CEscherichia colisalicylic acid0.0920.0312.93PMID 31894971Open
EntEvariant Y236F/S240C/V339I/N340CEscherichia coli2,3-dihydroxybenzoic acid0.1470.4170.35PMID 31894971Open
EntEvariant Y236F/S240C/V339I/N340CEscherichia colisalicylic acid0.1170.0244.88PMID 31894971Open
EntEvariant Y236F/S240C/V339L/N340CEscherichia colisalicylic acid0.1020.5330.18PMID 31894971Open