Values CatLog read from this paper
Kinetic mechanism of uracil phosphoribosyltransferase from Escherichia coli and catalytic importance of the conserved proline in the PRPP binding site
PMID 10079076doi:10.1021/bi982279qOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (14 values) from this paper: uracil phosphoribosyltransferase (UPRTase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| uracil phosphoribosyltransferase (UPRTase) | Escherichia coli | 5-phosphoribosyl-α-1-pyrophosphate20 mM Tris/HCl; uracil | — | 0.058 | 47 | pH 8.5, 37 °C | Open record: uracil phosphoribosyltransferase (UPRTase), 5-phosphoribosyl-α-1-pyrophosphate |
| uracil phosphoribosyltransferase (UPRTase) | Escherichia coli | 5-phosphoribosyl-α-1-pyrophosphate20 mM Tris-HCl; Michaelis-Menten fit (eq 5) | — | 0.073 | — | pH 8.5, 37 °C | Open record: uracil phosphoribosyltransferase (UPRTase), 5-phosphoribosyl-α-1-pyrophosphate |
| uracil phosphoribosyltransferase (UPRTase) | Escherichia coli | UMP | 0.024 | — | — | pH 8.5, 37 °C | Open record: uracil phosphoribosyltransferase (UPRTase), UMP |
| uracil phosphoribosyltransferase (UPRTase) | Escherichia coli | uracil20 mM Tris/HCl; PRPP | 2.7 | 5.3 × 10⁻⁴ | 5.1 × 10³ | pH 8.5, 37 °C | Open record: uracil phosphoribosyltransferase (UPRTase), uracil |
| uracil phosphoribosyltransferase (UPRTase) | Escherichia coli | uracil20 mM Tris-HCl; Michaelis-Menten fit (eq 5) | — | 6.3 × 10⁻⁴ | — | pH 8.5, 37 °C | Open record: uracil phosphoribosyltransferase (UPRTase), uracil |
| uracil phosphoribosyltransferase (UPRTase)variant P131D | Escherichia coli | 5-phosphoribosyl-α-1-pyrophosphate | — | 0.0013 | 38 | pH 8.5, 37 °C | Open record: uracil phosphoribosyltransferase (UPRTase) variant P131D, 5-phosphoribosyl-α-1-pyrophosphate |
| uracil phosphoribosyltransferase (UPRTase)variant P131D | Escherichia coli | UMP | 4.1 × 10⁻⁴ | — | — | pH 8.5, 37 °C | Open record: uracil phosphoribosyltransferase (UPRTase) variant P131D, UMP |
| uracil phosphoribosyltransferase (UPRTase)variant P131D | Escherichia coli | uracil | 0.05 | 0.06 | 0.83 | pH 8.5, 37 °C | Open record: uracil phosphoribosyltransferase (UPRTase) variant P131D, uracil |