CatLogenzyme kinetics catalog

Values CatLog read from this paper

Systematic mutations of highly conserved His49 and carboxyl-terminal of recombinant porcine liver NADH-cytochrome b5 reductase solubilized domain

PMID 10082957doi:10.1016/s0167-4838(99)00008-4Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 27 records (51 values) from this paper: NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R) from Sus scrofa.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 24 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.6.2.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)Sus scrofaNADH3900.00162.4 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)Sus scrofaNADHrecombinant solubilized catalytic domain (rPb5R)5700.00153.8 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)Sus scrofapotassium ferricyanide10 mM potassium phosphate; 100 µM NADH as electron donor6300.00252.5 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)Sus scrofapotassium ferricyanide10 mM potassium phosphate; 100 µM NADH as electron donor8100.00282.9 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)Sus scrofapotassium ferricyanide100 mM potassium phosphate (higher ionic strength)—0.0091—pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)Sus scrofatrypsin-solubilized porcine liver cytochrome b55100.00885.8 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)Sus scrofatrypsin-solubilized porcine liver cytochrome b5recombinant solubilized catalytic domain (rPb5R)5600.00916.2 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)G273 insertion (G273-Pb5R, Gly273 added after C-terminal Phe272)Sus scrofaNADH536.4 × 10⁻⁴8.3 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)G273 insertion (G273-Pb5R, Gly273 added after C-terminal Phe272)Sus scrofapotassium ferricyanide760.00521.5 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)G273 insertion (G273-Pb5R, Gly273 added after C-terminal Phe272)Sus scrofatrypsin-solubilized porcine liver cytochrome b5570.00252.3 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49A (A49-Pb5R)Sus scrofaNADH4400.00192.3 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49A (A49-Pb5R)Sus scrofapotassium ferricyanide4700.00281.7 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49A (A49-Pb5R)Sus scrofatrypsin-solubilized porcine liver cytochrome b53800.00894.3 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49E (E49-Pb5R)Sus scrofaNADH773 × 10⁻⁴2.6 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49E (E49-Pb5R)Sus scrofapotassium ferricyanide10 mM potassium phosphate; 100 µM NADH as electron donor1100.00225.0 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49E (E49-Pb5R)Sus scrofapotassium ferricyanide100 mM potassium phosphate (higher ionic strength)—0.0089—pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49E (E49-Pb5R)Sus scrofatrypsin-solubilized porcine liver cytochrome b51600.035.3 × 10³ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49K (K49-Pb5R)Sus scrofaNADH7100.017.1 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49K (K49-Pb5R)Sus scrofapotassium ferricyanide10 mM potassium phosphate; 100 µM NADH as electron donor4906 × 10⁻⁴8.2 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49K (K49-Pb5R)Sus scrofapotassium ferricyanide100 mM potassium phosphate (higher ionic strength)—0.0079—pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49K (K49-Pb5R)Sus scrofatrypsin-solubilized porcine liver cytochrome b54200.00311.4 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49Y (Y49-Pb5R)Sus scrofaNADH7500.00272.8 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49Y (Y49-Pb5R)Sus scrofapotassium ferricyanide7300.00262.8 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)variant H49Y (Y49-Pb5R)Sus scrofatrypsin-solubilized porcine liver cytochrome b55800.0115.3 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)ΔF272 (ΔF272-Pb5R, C-terminal Phe272 deleted)Sus scrofaNADH3108.4 × 10⁻⁴3.7 × 10⁵ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)ΔF272 (ΔF272-Pb5R, C-terminal Phe272 deleted)Sus scrofapotassium ferricyanide4000.00527.7 × 10⁴ calc.pH 7, 25 °COpen
NADH-cytochrome b5 reductase (solubilized catalytic domain, Pb5R)ΔF272 (ΔF272-Pb5R, C-terminal Phe272 deleted)Sus scrofatrypsin-solubilized porcine liver cytochrome b54500.0172.6 × 10⁴ calc.pH 7, 25 °COpen