Values CatLog read from this paper
Identification of the reactive cysteine residue (Cys227) in human carbonyl reductase
PMID 10091578doi:10.1046/j.1432-1327.1999.00089.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 26 records (44 values) from this paper: carbonyl reductase from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 18 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| carbonyl reductase | Homo sapiens | 2-methyl-1,4-naphthoquinone | 6.7 | 0.022 | 300 calc. | pH 7, 25 °C | Open record: carbonyl reductase, 2-methyl-1,4-naphthoquinone |
| carbonyl reductase | Homo sapiens | daunorubicin | 1.6 | 0.16 | 10 calc. | pH 7, 25 °C | Open record: carbonyl reductase, daunorubicin |
| carbonyl reductase | Homo sapiens | NADPH | — | 0.003 | — | pH 7, 25 °C | Open record: carbonyl reductase, NADPH |
| carbonyl reductase | Homo sapiens | phenyl(pyridin-4-yl)methanone | 6.3 | 0.29 | 22 calc. | pH 7, 25 °C | Open record: carbonyl reductase, phenyl(pyridin-4-yl)methanone |
| carbonyl reductasevariant C122A | Homo sapiens | 2-methyl-1,4-naphthoquinone | 5.9 | 0.022 | 270 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C122A, 2-methyl-1,4-naphthoquinone |
| carbonyl reductasevariant C122A | Homo sapiens | daunorubicin | 1.7 | 0.16 | 11 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C122A, daunorubicin |
| carbonyl reductasevariant C122A | Homo sapiens | NADPH | — | 0.003 | — | pH 7, 25 °C | Open record: carbonyl reductase variant C122A, NADPH |
| carbonyl reductasevariant C122A | Homo sapiens | phenyl(pyridin-4-yl)methanone | 4.7 | 0.33 | 14 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C122A, phenyl(pyridin-4-yl)methanone |
| carbonyl reductasevariant C150A | Homo sapiens | 2-methyl-1,4-naphthoquinone | 6.6 | 0.024 | 280 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C150A, 2-methyl-1,4-naphthoquinone |
| carbonyl reductasevariant C150A | Homo sapiens | daunorubicin | 1.4 | 0.18 | 7.8 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C150A, daunorubicin |
| carbonyl reductasevariant C150A | Homo sapiens | NADPH | — | 0.003 | — | pH 7, 25 °C | Open record: carbonyl reductase variant C150A, NADPH |
| carbonyl reductasevariant C150A | Homo sapiens | phenyl(pyridin-4-yl)methanone | 5.4 | 0.27 | 20 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C150A, phenyl(pyridin-4-yl)methanone |
| carbonyl reductasevariant C226A | Homo sapiens | 2-methyl-1,4-naphthoquinone | 8.9 | 0.2 | 44 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C226A, 2-methyl-1,4-naphthoquinone |
| carbonyl reductasevariant C226A | Homo sapiens | daunorubicin | 0.7 | 0.45 | 1.6 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C226A, daunorubicin |
| carbonyl reductasevariant C226A | Homo sapiens | NADPH | — | 0.009 | — | pH 7, 25 °C | Open record: carbonyl reductase variant C226A, NADPH |
| carbonyl reductasevariant C226A | Homo sapiens | phenyl(pyridin-4-yl)methanone | 5 | — | — | pH 7, 25 °C | Open record: carbonyl reductase variant C226A, phenyl(pyridin-4-yl)methanone |
| carbonyl reductasevariant C227A | Homo sapiens | 2-methyl-1,4-naphthoquinone | 0.5 | 0.87 | 0.57 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C227A, 2-methyl-1,4-naphthoquinone |
| carbonyl reductasevariant C227A | Homo sapiens | daunorubicin | 0.12 | 0.44 | 0.27 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C227A, daunorubicin |
| carbonyl reductasevariant C227A | Homo sapiens | NADPH | — | 0.013 | — | pH 7, 25 °C | Open record: carbonyl reductase variant C227A, NADPH |
| carbonyl reductasevariant C227S | Homo sapiens | 2-methyl-1,4-naphthoquinone | 0.8 | 0.8 | 1.0 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C227S, 2-methyl-1,4-naphthoquinone |
| carbonyl reductasevariant C227S | Homo sapiens | daunorubicin | 0.55 | 0.47 | 1.2 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C227S, daunorubicin |
| carbonyl reductasevariant C227S | Homo sapiens | NADPH | — | 0.012 | — | pH 7, 25 °C | Open record: carbonyl reductase variant C227S, NADPH |
| carbonyl reductasevariant C26A | Homo sapiens | 2-methyl-1,4-naphthoquinone | 8.4 | 0.025 | 340 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C26A, 2-methyl-1,4-naphthoquinone |
| carbonyl reductasevariant C26A | Homo sapiens | daunorubicin | 1.2 | 0.14 | 8.6 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C26A, daunorubicin |
| carbonyl reductasevariant C26A | Homo sapiens | NADPH | — | 0.003 | — | pH 7, 25 °C | Open record: carbonyl reductase variant C26A, NADPH |
| carbonyl reductasevariant C26A | Homo sapiens | phenyl(pyridin-4-yl)methanone | 3.7 | 0.23 | 16 calc. | pH 7, 25 °C | Open record: carbonyl reductase variant C26A, phenyl(pyridin-4-yl)methanone |