Values CatLog read from this paper
Kinetic studies on the enzyme (S)-hydroxynitrile lyase from hevea brasiliensis using initial rate methods and progress curve analysis
PMID 10099509doi:10.1002/(sici)1097-0290(19990105)62:1<20::aid-bit3>3.0.co;2-iOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (10 values) from this paper: (S)-hydroxynitrile lyase (HbHnl) from Hevea brasiliensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| (S)-hydroxynitrile lyase (HbHnl) | Hevea brasiliensis | benzaldehyde | 3436 | 5.2 | 660 calc. | pH 5, 25 °C | Open record: (S)-hydroxynitrile lyase (HbHnl), benzaldehyde |
| (S)-hydroxynitrile lyase (HbHnl) | Hevea brasiliensis | benzaldehydekcat calculated from the enzyme concentration | 2111 | 4.51 | 468 calc. | pH 5, 25 °C | Open record: (S)-hydroxynitrile lyase (HbHnl), benzaldehyde |
| (S)-hydroxynitrile lyase (HbHnl) | Hevea brasiliensis | hydrogen cyanide | — | 350 | — | pH 5, 25 °C | Open record: (S)-hydroxynitrile lyase (HbHnl), hydrogen cyanide |
| (S)-hydroxynitrile lyase (HbHnl) | Hevea brasiliensis | hydrogen cyanideKm calculated from the enzyme concentration | — | 294.8 | — | pH 5, 25 °C | Open record: (S)-hydroxynitrile lyase (HbHnl), hydrogen cyanide |
| (S)-hydroxynitrile lyase (HbHnl) | Hevea brasiliensis | racemic mandelonitrile | 64 | 3.1 | 21 calc. | pH 5, 25 °C | Open record: (S)-hydroxynitrile lyase (HbHnl), racemic mandelonitrile |
| (S)-hydroxynitrile lyase (HbHnl) | Hevea brasiliensis | racemic mandelonitrilekcat calculated from the enzyme concentration | 131 | 2.93 | 44.7 calc. | pH 5, 25 °C | Open record: (S)-hydroxynitrile lyase (HbHnl), racemic mandelonitrile |