Values CatLog read from this paper
Engineered glycolytic glyceraldehyde-3-phosphate dehydrogenase binds the anti conformation of NAD+ nicotinamide but does not experience A-specific hydride transfer
PMID 10190977doi:10.1006/abbi.1999.1116Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (20 values) from this paper: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Escherichia coli | D-glyceraldehyde 3-phosphate | 268 | 0.89 | 300 calc. | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH), D-glyceraldehyde 3-phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Escherichia coli | NAD+ | — | 0.045 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH), NAD+ |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Escherichia coli | phosphate | — | 0.53 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant N313T | Escherichia coli | D-glyceraldehyde 3-phosphate | 4.9 | 0.42 | 12 calc. | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant N313T, D-glyceraldehyde 3-phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant N313T | Escherichia coli | NAD+ | — | 0.19 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant N313T, NAD+ |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant N313T | Escherichia coli | phosphate | — | 20 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant N313T, phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant N313T/Y317G | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.03 | 0.29 | 0.10 calc. | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant N313T/Y317G, D-glyceraldehyde 3-phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant N313T/Y317G | Escherichia coli | NAD+ | — | 0.058 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant N313T/Y317G, NAD+ |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant N313T/Y317G | Escherichia coli | phosphate | — | 6.9 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant N313T/Y317G, phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant Y317A | Escherichia coli | D-glyceraldehyde 3-phosphate | 65 | 0.88 | 74 calc. | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant Y317A, D-glyceraldehyde 3-phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant Y317A | Escherichia coli | NAD+ | — | 0.05 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant Y317A, NAD+ |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant Y317A | Escherichia coli | phosphate | — | 4.8 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant Y317A, phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant Y317G | Escherichia coli | D-glyceraldehyde 3-phosphate | 30 | 2.05 | 15 calc. | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant Y317G, D-glyceraldehyde 3-phosphate |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant Y317G | Escherichia coli | NAD+ | — | 0.19 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant Y317G, NAD+ |
| glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH)variant Y317G | Escherichia coli | phosphate | — | 35.1 | — | pH 8.9, 22 °C | Open record: glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) variant Y317G, phosphate |