Values CatLog read from this paper

Identification of residues of Escherichia coli phosphofructokinase that contribute to nucleotide binding and specificity

PMID 10194349doi:10.1021/bi982940qOpen in Browse

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CatLog read 81 records (81 values) from this paper: phosphofructo-1-kinase (PFK) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.1.11

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
phosphofructo-1-kinase (PFK)	Escherichia coli	adenosine 5'-triphosphate	—	0.21	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK), adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)	Escherichia coli	cytidine 5'-triphosphate	—	11	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK), cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)	Escherichia coli	D-fructose 6-phosphate	—	0.107	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK), D-fructose 6-phosphate
phosphofructo-1-kinase (PFK)	Escherichia coli	guanosine 5'-triphosphate	—	4.3	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK), guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)	Escherichia coli	inosine 5'-triphosphate	—	2.2	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK), inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)	Escherichia coli	uridine 5'-triphosphate	—	5.1	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK), uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76A	Escherichia coli	adenosine 5'-triphosphate	—	13.1	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76A, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76A	Escherichia coli	cytidine 5'-triphosphate	—	3.6	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76A, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76A	Escherichia coli	guanosine 5'-triphosphate	—	9.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76A, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76A	Escherichia coli	inosine 5'-triphosphate	—	7.7	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76A, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76A	Escherichia coli	uridine 5'-triphosphate	—	32	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76A, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76W	Escherichia coli	adenosine 5'-triphosphate	—	0.17	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76W, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76W	Escherichia coli	cytidine 5'-triphosphate	—	1.43	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76W, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76W	Escherichia coli	guanosine 5'-triphosphate	—	7.1	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76W, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76W	Escherichia coli	inosine 5'-triphosphate	—	6.8	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76W, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76W	Escherichia coli	uridine 5'-triphosphate	—	11.9	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76W, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76Y	Escherichia coli	adenosine 5'-triphosphate	—	1.14	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76Y, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76Y	Escherichia coli	cytidine 5'-triphosphate	—	66	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76Y, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76Y	Escherichia coli	guanosine 5'-triphosphate	—	10.7	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76Y, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76Y	Escherichia coli	inosine 5'-triphosphate	—	14.3	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76Y, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant F76Y	Escherichia coli	uridine 5'-triphosphate	—	27	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant F76Y, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111A	Escherichia coli	adenosine 5'-triphosphate	—	0.43	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111A, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111A	Escherichia coli	cytidine 5'-triphosphate	—	2.2	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111A, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111A	Escherichia coli	guanosine 5'-triphosphate	—	0.62	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111A, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111A	Escherichia coli	inosine 5'-triphosphate	—	1.65	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111A, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111A	Escherichia coli	uridine 5'-triphosphate	—	7.7	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111A, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111E	Escherichia coli	adenosine 5'-triphosphate	—	1.69	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111E, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111E	Escherichia coli	cytidine 5'-triphosphate	—	16.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111E, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111E	Escherichia coli	guanosine 5'-triphosphate	—	1.67	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111E, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111E	Escherichia coli	inosine 5'-triphosphate	—	5.4	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111E, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R111E	Escherichia coli	uridine 5'-triphosphate	—	70	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R111E, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77A	Escherichia coli	adenosine 5'-triphosphate	—	0.52	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77A, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77A	Escherichia coli	cytidine 5'-triphosphate	—	60	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77A, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77A	Escherichia coli	guanosine 5'-triphosphate	—	5.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77A, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77A	Escherichia coli	inosine 5'-triphosphate	—	19.7	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77A, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77A	Escherichia coli	uridine 5'-triphosphate	—	21	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77A, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77D	Escherichia coli	adenosine 5'-triphosphate	—	4.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77D, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77D	Escherichia coli	cytidine 5'-triphosphate	—	34	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77D, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77D	Escherichia coli	guanosine 5'-triphosphate	—	8.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77D, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77D	Escherichia coli	inosine 5'-triphosphate	—	21	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77D, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77D	Escherichia coli	uridine 5'-triphosphate	—	19	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77D, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77E	Escherichia coli	adenosine 5'-triphosphate	—	2.2	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77E, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77E	Escherichia coli	cytidine 5'-triphosphate	—	12.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77E, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77E	Escherichia coli	guanosine 5'-triphosphate	—	6.3	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77E, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77E	Escherichia coli	inosine 5'-triphosphate	—	2.6	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77E, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77E	Escherichia coli	uridine 5'-triphosphate	—	44	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77E, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77L	Escherichia coli	adenosine 5'-triphosphate	—	0.52	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77L, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77L	Escherichia coli	cytidine 5'-triphosphate	—	9.9	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77L, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77L	Escherichia coli	guanosine 5'-triphosphate	—	8.6	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77L, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77L	Escherichia coli	inosine 5'-triphosphate	—	11.9	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77L, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R77L	Escherichia coli	uridine 5'-triphosphate	—	4.4	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R77L, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82A	Escherichia coli	adenosine 5'-triphosphate	—	5.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82A, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82A	Escherichia coli	cytidine 5'-triphosphate	—	1.64	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82A, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82A	Escherichia coli	guanosine 5'-triphosphate	—	2.1	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82A, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82A	Escherichia coli	inosine 5'-triphosphate	—	14.9	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82A, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82A	Escherichia coli	uridine 5'-triphosphate	—	15	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82A, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82E	Escherichia coli	adenosine 5'-triphosphate	—	4.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82E, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82E	Escherichia coli	cytidine 5'-triphosphate	—	50	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82E, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82E	Escherichia coli	guanosine 5'-triphosphate	—	1.43	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82E, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82E	Escherichia coli	inosine 5'-triphosphate	—	6.2	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82E, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant R82E	Escherichia coli	uridine 5'-triphosphate	—	70	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant R82E, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41A	Escherichia coli	adenosine 5'-triphosphate	—	9.6	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41A, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41A	Escherichia coli	cytidine 5'-triphosphate	—	13	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41A, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41A	Escherichia coli	guanosine 5'-triphosphate	—	8.8	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41A, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41A	Escherichia coli	inosine 5'-triphosphate	—	11	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41A, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41A	Escherichia coli	uridine 5'-triphosphate	—	36	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41A, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41F	Escherichia coli	adenosine 5'-triphosphate	—	0.17	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41F, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41F	Escherichia coli	cytidine 5'-triphosphate	—	7.2	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41F, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41F	Escherichia coli	guanosine 5'-triphosphate	—	3.7	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41F, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41F	Escherichia coli	inosine 5'-triphosphate	—	2.3	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41F, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41F	Escherichia coli	uridine 5'-triphosphate	—	11.6	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41F, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41L	Escherichia coli	adenosine 5'-triphosphate	—	0.38	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41L, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41L	Escherichia coli	cytidine 5'-triphosphate	—	8.9	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41L, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41L	Escherichia coli	guanosine 5'-triphosphate	—	6.4	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41L, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41L	Escherichia coli	inosine 5'-triphosphate	—	5.7	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41L, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41L	Escherichia coli	uridine 5'-triphosphate	—	16.2	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41L, uridine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41W	Escherichia coli	adenosine 5'-triphosphate	—	0.6	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41W, adenosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41W	Escherichia coli	cytidine 5'-triphosphate	—	8.8	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41W, cytidine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41W	Escherichia coli	guanosine 5'-triphosphate	—	5.1	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41W, guanosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41W	Escherichia coli	inosine 5'-triphosphate	—	5.3	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41W, inosine 5'-triphosphate
phosphofructo-1-kinase (PFK)variant Y41W	Escherichia coli	uridine 5'-triphosphate	—	19.5	—	pH 7.2, 30 °C	Open record: phosphofructo-1-kinase (PFK) variant Y41W, uridine 5'-triphosphate