Values CatLog read from this paper
Electron transfer from quinohemoprotein alcohol dehydrogenase to blue copper protein azurin in the alcohol oxidase respiratory chain of Pseudomonas putida HK5
PMID 10320337doi:10.1021/bi990121fOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 5 records (10 values) from this paper: quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH) from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH) | Pseudomonas putida | azurin from Pseudomonas aeruginosa (commercial, Sigma) | 25.6 | 0.202 | 127 calc. | pH 8, 25 °C | Open record: quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH), azurin from Pseudomonas aeruginosa (commercial, Sigma) |
| quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH) | Pseudomonas putida | azurin from Pseudomonas putida HK5 (blue copper protein)30 mM Tris-HCl (pH 8.0); no KCl | 71.8 | 0.1 | 720 calc. | pH 8, 25 °C | Open record: quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH), azurin from Pseudomonas putida HK5 (blue copper protein) |
| quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH) | Pseudomonas putida | azurin from Pseudomonas putida HK5 (blue copper protein)30 mM Tris-HCl (pH 8.0) and 0.1 M KCl | 60.5 | 0.051 | 1.2 × 10³ calc. | pH 8, 25 °C | Open record: quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH), azurin from Pseudomonas putida HK5 (blue copper protein) |
| quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH) | Pseudomonas putida | azurin from Pseudomonas putida HK5 (blue copper protein)30 mM Tris-HCl (pH 8.0) and 0.5 M KCl | 48 | 0.025 | 1.9 × 10³ calc. | pH 8, 25 °C | Open record: quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH), azurin from Pseudomonas putida HK5 (blue copper protein) |
| quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH) | Pseudomonas putida | potassium ferricyanide (potassium hexacyanoferrate(III)) | 52.3 | 0.095 | 550 calc. | pH 8, 25 °C | Open record: quinohemoprotein alcohol dehydrogenase ADH IIB (type II ADH), potassium ferricyanide (potassium hexacyanoferrate(III)) |