CatLogenzyme kinetics catalog

Values CatLog read from this paper

Steady-state kinetic characterization of RB69 DNA polymerase mutants that affect dNTP incorporation

PMID 10387055doi:10.1021/bi990653wOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 39 records (78 values) from this paper: RB69 DNA polymerase (gp43) from bacteriophage RB69.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 39 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.7.7

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
RB69 DNA polymerase (gp43)variant D222A/D327Abacteriophage RB692'-deoxyadenosine 5'-triphosphate0.223.4 × 10⁻⁴650 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant D222A/D327Abacteriophage RB692'-deoxycytidine 5'-triphosphate15.7 × 10⁻⁴1.8 × 10³ calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-triphosphate0.371.7 × 10⁻⁴2.2 × 10³ calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant D222A/D327Abacteriophage RB692'-deoxyuridine 5'-triphosphate0.780.0013600 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant D222A/D327Abacteriophage RB69thymidine 5'-triphosphate0.570.0024240 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K483A/D222A/D327Abacteriophage RB692'-deoxyadenosine 5'-triphosphate0.233.3 × 10⁻⁴700 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K483A/D222A/D327Abacteriophage RB692'-deoxycytidine 5'-triphosphate0.485.3 × 10⁻⁴910 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K483A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-triphosphate0.473.7 × 10⁻⁴1.3 × 10³ calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K483A/D222A/D327Abacteriophage RB692'-deoxyuridine 5'-triphosphate0.330.0023140 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K483A/D222A/D327Abacteriophage RB69thymidine 5'-triphosphate0.50.0042120 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K486A/D222A/D327Abacteriophage RB692'-deoxyadenosine 5'-triphosphate0.0060.0061.0 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K486A/D222A/D327Abacteriophage RB692'-deoxycytidine 5'-triphosphate0.0930.0175.5 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K486A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-diphosphate1.4 × 10⁻⁴0.00220.064 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K486A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-triphosphate0.00190.00660.29 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K486A/D222A/D327Abacteriophage RB692'-deoxyuridine 5'-triphosphate2.2 × 10⁻⁴0.00220.10 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K486A/D222A/D327Abacteriophage RB69thymidine 5'-triphosphate4.4 × 10⁻⁴0.00580.076 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K560A/D222A/D327Abacteriophage RB692'-deoxyadenosine 5'-triphosphate0.20.12.0 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K560A/D222A/D327Abacteriophage RB692'-deoxycytidine 5'-triphosphate0.0650.0193.4 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K560A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-diphosphate7.8 × 10⁻⁵0.0030.026 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K560A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-triphosphate0.170.0513.3 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K560A/D222A/D327Abacteriophage RB692'-deoxyuridine 5'-triphosphate0.30.380.79 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant K560A/D222A/D327Abacteriophage RB69thymidine 5'-triphosphate2.13.30.64 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant N564A/D222A/D327Abacteriophage RB692'-deoxyadenosine 5'-triphosphate0.0830.018.3 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant N564A/D222A/D327Abacteriophage RB692'-deoxycytidine 5'-triphosphate0.160.006325 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant N564A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-diphosphate2.7 × 10⁻⁴8.3 × 10⁻⁴0.33 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant N564A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-triphosphate0.180.0017110 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant N564A/D222A/D327Abacteriophage RB692'-deoxyuridine 5'-triphosphate0.330.02513 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant N564A/D222A/D327Abacteriophage RB69thymidine 5'-triphosphate0.420.0646.6 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant R482A/D222A/D327Abacteriophage RB692'-deoxyadenosine 5'-triphosphate0.00530.00640.83 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant R482A/D222A/D327Abacteriophage RB692'-deoxycytidine 5'-triphosphate0.0340.0152.3 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant R482A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-diphosphate3.2 × 10⁻⁴0.00170.19 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant R482A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-triphosphate0.00530.00511.0 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant R482A/D222A/D327Abacteriophage RB692'-deoxyuridine 5'-triphosphate0.00110.00270.41 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant R482A/D222A/D327Abacteriophage RB69thymidine 5'-triphosphate0.00180.00520.35 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant Y567A/D222A/D327Abacteriophage RB692'-deoxyadenosine 5'-triphosphate0.065.9 × 10⁻⁴100 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant Y567A/D222A/D327Abacteriophage RB692'-deoxycytidine 5'-triphosphate0.154.2 × 10⁻⁴360 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant Y567A/D222A/D327Abacteriophage RB692'-deoxyguanosine 5'-triphosphate0.622 × 10⁻⁴3.1 × 10³ calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant Y567A/D222A/D327Abacteriophage RB692'-deoxyuridine 5'-triphosphate10.0024420 calc.pH 8.8Open
RB69 DNA polymerase (gp43)variant Y567A/D222A/D327Abacteriophage RB69thymidine 5'-triphosphate1.30.004320 calc.pH 8.8Open