Values CatLog read from this paper
Identification of specific residues involved in substrate discrimination in two plant O-methyltransferases
PMID 10415125doi:10.1006/abbi.1999.1304Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (33 values) from this paper: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT), S-adenosyl-L-methionine:caffeic acid O-methyltransferase (COMT) from Clarkia breweri.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT) | Clarkia breweri | isoeugenol | 0.08 | 0.074 | 1.1 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT), isoeugenol |
| S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT)variant F130L/L131C | Clarkia breweri | isoeugenol | 0.33 | 0.019 | 17.4 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT) variant F130L/L131C, isoeugenol |
| S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT)variant F130L/L131C/N164T/E165A | Clarkia breweri | isoeugenol | 0.002 | 0.008 | 0.25 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT) variant F130L/L131C/N164T/E165A, isoeugenol |
| S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT)variant F130L/L131C/T133M/A134N/T135Q | Clarkia breweri | caffeic acid | 0.016 | 0.109 | 0.15 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT) variant F130L/L131C/T133M/A134N/T135Q, caffeic acid |
| S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT)variant F130L/L131C/T133M/A134N/T135Q/N164T/E165A | Clarkia breweri | caffeic acid | 0.012 | 0.103 | 0.12 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT) variant F130L/L131C/T133M/A134N/T135Q/N164T/E165A, caffeic acid |
| S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT)variant N164T/E165A | Clarkia breweri | isoeugenol | 0.025 | 0.018 | 1.4 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT) variant N164T/E165A, isoeugenol |
| S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT)variant T133M/A134N/T135Q | Clarkia breweri | isoeugenol | 0.002 | 0.087 | 0.02 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT) variant T133M/A134N/T135Q, isoeugenol |
| S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT)variant T133M/A134N/T135Q/N164T/E165A | Clarkia breweri | caffeic acid | 0.001 | 0.145 | 0.007 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase (IEMT) variant T133M/A134N/T135Q/N164T/E165A, caffeic acid |
| S-adenosyl-L-methionine:caffeic acid O-methyltransferase (COMT) | Clarkia breweri | caffeic acid | 0.26 | 0.076 | 3.4 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:caffeic acid O-methyltransferase (COMT), caffeic acid |
| S-adenosyl-L-methionine:caffeic acid O-methyltransferase (COMT)variant L130F/C131L/M133T/N134A/Q135T | Clarkia breweri | eugenol | 0.006 | 0.08 | 0.07 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:caffeic acid O-methyltransferase (COMT) variant L130F/C131L/M133T/N134A/Q135T, eugenol |
| S-adenosyl-L-methionine:caffeic acid O-methyltransferase (COMT)variant L130F/C131L/M133T/N134A/Q135T/T164N/A165E | Clarkia breweri | isoeugenol | 0.006 | 0.056 | 0.01 | pH 7.5, 30 °C | Open record: S-adenosyl-L-methionine:caffeic acid O-methyltransferase (COMT) variant L130F/C131L/M133T/N134A/Q135T/T164N/A165E, isoeugenol |