Values CatLog read from this paper
Crystal structure of the bifunctional N-acetylglucosamine 1-phosphate uridyltransferase from Escherichia coli: a paradigm for the related pyrophosphorylase superfamily
PMID 10428949doi:10.1093/emboj/18.15.4096Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (23 values) from this paper: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) | Escherichia coli | glucosamine 1-phosphate | 1.5 × 10³ | — | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase), glucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) | Escherichia coli | glucosamine 1-phosphateN-terminal His6-tagged (pFP3, Ni affinity purified) | 1.35 × 10³ | — | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase), glucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) | Escherichia coli | N-acetylglucosamine 1-phosphate | 350 | 0.07 | 5.0 × 10³ calc. | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase), N-acetylglucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) | Escherichia coli | N-acetylglucosamine 1-phosphateN-terminal His6-tagged (pFP3, Ni affinity purified) | 330 | 0.07 | 4.7 × 10³ calc. | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase), N-acetylglucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) | Escherichia coli | uridine 5'-triphosphate | — | 0.1 | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase), uridine 5'-triphosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) | Escherichia coli | uridine 5'-triphosphateN-terminal His6-tagged (pFP3, Ni affinity purified) | — | 0.1 | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase), uridine 5'-triphosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant G14A | Escherichia coli | glucosamine 1-phosphate | 811 | — | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant G14A, glucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant G14A | Escherichia coli | N-acetylglucosamine 1-phosphate | 40 | 0.1 | 400 calc. | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant G14A, N-acetylglucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant G14A | Escherichia coli | uridine 5'-triphosphate | — | 0.08 | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant G14A, uridine 5'-triphosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant GlmU-Tr (residues 1-331) | Escherichia coli | N-acetylglucosamine 1-phosphate | 136 | 0.08 | 1.7 × 10³ calc. | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant GlmU-Tr (residues 1-331), N-acetylglucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant GlmU-Tr (residues 1-331) | Escherichia coli | uridine 5'-triphosphate | — | 0.1 | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant GlmU-Tr (residues 1-331), uridine 5'-triphosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant K25A | Escherichia coli | glucosamine 1-phosphate | 1167 | — | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant K25A, glucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant K25A | Escherichia coli | N-acetylglucosamine 1-phosphate | 46 | 0.075 | 610 calc. | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant K25A, N-acetylglucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant K25A | Escherichia coli | uridine 5'-triphosphate | — | 0.17 | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant K25A, uridine 5'-triphosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant R18A | Escherichia coli | glucosamine 1-phosphate | 1045 | — | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant R18A, glucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant R18A | Escherichia coli | N-acetylglucosamine 1-phosphate | 0.06 | 0.06 | 1.0 calc. | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant R18A, N-acetylglucosamine 1-phosphate |
| GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase)variant R18A | Escherichia coli | uridine 5'-triphosphate | — | 0.26 | — | pH 8.2, 37 °C | Open record: GlmU (bifunctional glucosamine-1-phosphate acetyltransferase / N-acetylglucosamine-1-phosphate uridyltransferase) variant R18A, uridine 5'-triphosphate |