CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of catalytically essential residues in Escherichia coli esterase by site-directed mutagenesis

PMID 10431819doi:10.1016/s0014-5793(99)00813-3Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 7 records (14 values) from this paper: Escherichia coli esterase (EcE) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
Escherichia coli esterase (EcE)variant D164AEscherichia colip-nitrophenyl butyrate0.00710.0070 calc.pH 7.1, 25 °COpen
Escherichia coli esterase (EcE)variant D262AEscherichia colip-nitrophenyl butyrate0.0340.40.085 calc.pH 7.1, 25 °COpen
Escherichia coli esterase (EcE)variant D266AEscherichia colip-nitrophenyl butyrate3.40.56.8 calc.pH 7.1, 25 °COpen
Escherichia coli esterase (EcE)variant E128QEscherichia colip-nitrophenyl butyrate7.40.3521 calc.pH 7.1, 25 °COpen
Escherichia coli esterase (EcE)variant G163AEscherichia colip-nitrophenyl butyrate2.90.55.8 calc.pH 7.1, 25 °COpen
Escherichia coli esterase (EcE)variant G167AEscherichia colip-nitrophenyl butyrate49.4225 calc.pH 7.1, 25 °COpen
Escherichia coli esterase (EcE)variant H103NEscherichia colip-nitrophenyl butyrate12.10.2548 calc.pH 7.1, 25 °COpen