Values CatLog read from this paper
Effects on substrate profile by mutational substitutions at positions 164 and 179 of the class A TEM(pUC19) beta-lactamase from Escherichia coli
PMID 10438473doi:10.1074/jbc.274.33.23052Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 45 records (135 values) from this paper: TEM(pUC19) β-lactamase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| TEM(pUC19) β-lactamase | Escherichia coli | Ampicillin | 1.04 × 10³ | 0.027 | 3.9 × 10⁴ | Open record: TEM(pUC19) β-lactamase, Ampicillin | |
| TEM(pUC19) β-lactamase | Escherichia coli | Aztreonam | 0.4 | 0.55 | 0.74 | Open record: TEM(pUC19) β-lactamase, Aztreonam | |
| TEM(pUC19) β-lactamase | Escherichia coli | benzylpenicillin | 1.57 × 10³ | 0.12 | 1.3 × 10⁴ | Open record: TEM(pUC19) β-lactamase, benzylpenicillin | |
| TEM(pUC19) β-lactamase | Escherichia coli | Cefotaxime | 0.18 | 0.23 | 0.78 | Open record: TEM(pUC19) β-lactamase, Cefotaxime | |
| TEM(pUC19) β-lactamase | Escherichia coli | Ceftazidime | 0.008 | 0.2 | 0.04 | Open record: TEM(pUC19) β-lactamase, Ceftazidime | |
| TEM(pUC19) β-lactamase | Escherichia coli | Cephaloridine | 780 | 0.67 | 1.2 × 10³ | Open record: TEM(pUC19) β-lactamase, Cephaloridine | |
| TEM(pUC19) β-lactamase | Escherichia coli | Cephalosporin C | 33 | 0.95 | 35 | Open record: TEM(pUC19) β-lactamase, Cephalosporin C | |
| TEM(pUC19) β-lactamase | Escherichia coli | Cephalothin | 41 | 0.21 | 190 | Open record: TEM(pUC19) β-lactamase, Cephalothin | |
| TEM(pUC19) β-lactamase | Escherichia coli | Piperacillin | 770 | 0.07 | 1.1 × 10⁴ | Open record: TEM(pUC19) β-lactamase, Piperacillin | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | Ampicillin | 13 | 1.1 | 12 | Open record: TEM(pUC19) β-lactamase variant D179G, Ampicillin | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | Aztreonam | 0.06 | 0.34 | 0.18 | Open record: TEM(pUC19) β-lactamase variant D179G, Aztreonam | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | benzylpenicillin | 0.72 | 0.038 | 19 | Open record: TEM(pUC19) β-lactamase variant D179G, benzylpenicillin | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | Cefotaxime | 0.007 | 0.102 | 0.069 | Open record: TEM(pUC19) β-lactamase variant D179G, Cefotaxime | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | Ceftazidime | 0.041 | 0.096 | 0.43 | Open record: TEM(pUC19) β-lactamase variant D179G, Ceftazidime | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | Cephaloridine | 6 | 0.87 | 6.9 | Open record: TEM(pUC19) β-lactamase variant D179G, Cephaloridine | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | Cephalosporin C | 0.69 | 0.12 | 5.9 | Open record: TEM(pUC19) β-lactamase variant D179G, Cephalosporin C | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | Cephalothin | 0.31 | 0.041 | 7.4 | Open record: TEM(pUC19) β-lactamase variant D179G, Cephalothin | |
| TEM(pUC19) β-lactamasevariant D179G | Escherichia coli | Piperacillin | 0.43 | 0.031 | 14 | Open record: TEM(pUC19) β-lactamase variant D179G, Piperacillin | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | Ampicillin | 1.6 | 0.085 | 18 | Open record: TEM(pUC19) β-lactamase variant D179N, Ampicillin | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | Aztreonam | 0.06 | 0.22 | 0.25 | Open record: TEM(pUC19) β-lactamase variant D179N, Aztreonam | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | benzylpenicillin | 1.52 | 0.016 | 95 | Open record: TEM(pUC19) β-lactamase variant D179N, benzylpenicillin | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | Cefotaxime | 0.023 | 0.029 | 0.79 | Open record: TEM(pUC19) β-lactamase variant D179N, Cefotaxime | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | Ceftazidime | 0.042 | 0.11 | 0.38 | Open record: TEM(pUC19) β-lactamase variant D179N, Ceftazidime | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | Cephaloridine | 1.3 | 0.9 | 1.4 | Open record: TEM(pUC19) β-lactamase variant D179N, Cephaloridine | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | Cephalosporin C | 0.062 | 0.038 | 1.6 | Open record: TEM(pUC19) β-lactamase variant D179N, Cephalosporin C | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | Cephalothin | 0.21 | 0.048 | 4.2 | Open record: TEM(pUC19) β-lactamase variant D179N, Cephalothin | |
| TEM(pUC19) β-lactamasevariant D179N | Escherichia coli | Piperacillin | 0.74 | 0.055 | 13 | Open record: TEM(pUC19) β-lactamase variant D179N, Piperacillin | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | Ampicillin | 79 | 0.0071 | 1.1 × 10⁴ | Open record: TEM(pUC19) β-lactamase variant R164N, Ampicillin | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | Aztreonam | 6.7 | 0.54 | 12 | Open record: TEM(pUC19) β-lactamase variant R164N, Aztreonam | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | benzylpenicillin | 153 | 0.037 | 4.1 × 10³ | Open record: TEM(pUC19) β-lactamase variant R164N, benzylpenicillin | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | Cefotaxime | 5.9 | 0.257 | 23 | Open record: TEM(pUC19) β-lactamase variant R164N, Cefotaxime | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | Ceftazidime | 8 | 0.296 | 27 | Open record: TEM(pUC19) β-lactamase variant R164N, Ceftazidime | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | Cephaloridine | 65 | 0.089 | 730 | Open record: TEM(pUC19) β-lactamase variant R164N, Cephaloridine | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | Cephalosporin C | 10 | 0.85 | 12 | Open record: TEM(pUC19) β-lactamase variant R164N, Cephalosporin C | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | Cephalothin | 41 | 0.455 | 90 | Open record: TEM(pUC19) β-lactamase variant R164N, Cephalothin | |
| TEM(pUC19) β-lactamasevariant R164N | Escherichia coli | Piperacillin | 109 | 0.047 | 2.4 × 10³ | Open record: TEM(pUC19) β-lactamase variant R164N, Piperacillin | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | Ampicillin | 15 | 0.037 | 420 | Open record: TEM(pUC19) β-lactamase variant R164S, Ampicillin | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | Aztreonam | 1.5 | 0.635 | 2.4 | Open record: TEM(pUC19) β-lactamase variant R164S, Aztreonam | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | benzylpenicillin | 21.9 | 0.024 | 910 | Open record: TEM(pUC19) β-lactamase variant R164S, benzylpenicillin | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | Cefotaxime | 0.2 | 0.174 | 1.1 | Open record: TEM(pUC19) β-lactamase variant R164S, Cefotaxime | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | Ceftazidime | 1.4 | 0.27 | 5.2 | Open record: TEM(pUC19) β-lactamase variant R164S, Ceftazidime | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | Cephaloridine | 22 | 0.239 | 92 | Open record: TEM(pUC19) β-lactamase variant R164S, Cephaloridine | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | Cephalosporin C | 1.2 | 0.44 | 2.7 | Open record: TEM(pUC19) β-lactamase variant R164S, Cephalosporin C | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | Cephalothin | 3.5 | 0.18 | 19 | Open record: TEM(pUC19) β-lactamase variant R164S, Cephalothin | |
| TEM(pUC19) β-lactamasevariant R164S | Escherichia coli | Piperacillin | 11 | 0.022 | 500 | Open record: TEM(pUC19) β-lactamase variant R164S, Piperacillin |