CatLogenzyme kinetics catalog

Values CatLog read from this paper

The N-terminal anchor sequences of 11beta-hydroxysteroid dehydrogenases determine their orientation in the endoplasmic reticulum membrane

PMID 10497248doi:10.1074/jbc.274.40.28762Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (19 values) from this paper: 11beta-HSD1, 11beta-HSD2 from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
11beta-HSD1Homo sapiens11-dehydrocorticosterone—5.94 × 10⁻⁴—37 °COpen
11beta-HSD1Homo sapienscorticosteroneuntagged native sequence—3.88 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1Homo sapienscorticosteroneC-terminally FLAG-tagged (construct 1F)—4.11 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1Homo sapienscorticosteroneN-terminally FLAG-tagged (construct F1)—3.47 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1Homo sapienscorticosterone—1.63 × 10⁻⁴—37 °COpen
11beta-HSD1variant K5RHomo sapienscorticosterone—3.46 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1variant K5R/K6RHomo sapienscorticosterone—3.67 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1variant K5SHomo sapiens11-dehydrocorticosterone—5.45 × 10⁻⁴—37 °COpen
11beta-HSD1variant K5SHomo sapienscorticosterone—4.78 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1variant K5SHomo sapienscorticosterone—1.47 × 10⁻⁴—37 °COpen
11beta-HSD1variant K5S/K6SHomo sapienscorticosterone—4.77 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1variant K6RHomo sapienscorticosterone—6.24 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1variant K6SHomo sapienscorticosterone—4.58 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1variant Y18A/Y19A/Y20A/Y21AHomo sapienscorticosterone—3.28 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1variant Y18F/Y19F/Y20F/Y21FHomo sapienscorticosterone—3.96 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD1variant Y19A/Y21AHomo sapienscorticosterone—3.74 × 10⁻⁴—pH 7.4, 37 °COpen
11beta-HSD2Homo sapienscorticosteroneuntagged native sequence—5.5 × 10⁻⁶—pH 7.4, 37 °COpen
11beta-HSD2Homo sapienscorticosteroneC-terminally FLAG-tagged (construct 2F)—5.1 × 10⁻⁶—pH 7.4, 37 °COpen
11beta-HSD2Homo sapienscorticosteroneN-terminally FLAG-tagged (construct F2)—6.3 × 10⁻⁶—pH 7.4, 37 °COpen