CatLogenzyme kinetics catalog

Values CatLog read from this paper

Site-directed mutagenesis of the conserved residues in component I of Bacillus subtilis heptaprenyl diphosphate synthase

PMID 10545188doi:10.1021/bi9913653Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (42 values) from this paper: heptaprenyl diphosphate synthase (HepPP synthase; component I + component II) from Bacillus subtilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.5.1.30

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)Bacillus subtilis(E,E)-farnesyl diphosphate—0.0072—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)Bacillus subtilisisopentenyl diphosphate18.30.01671.10 × 10³ calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant D73ABacillus subtilis(E,E)-farnesyl diphosphate—0.0064—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant D73ABacillus subtilisisopentenyl diphosphate20.80.01611.29 × 10³ calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant D97ABacillus subtilis(E,E)-farnesyl diphosphate—0.0069—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant D97ABacillus subtilisisopentenyl diphosphate26.220.01561.68 × 10³ calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant E128VBacillus subtilis(E,E)-farnesyl diphosphate—0.0056—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant E128VBacillus subtilisisopentenyl diphosphate20.80.01721.21 × 10³ calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant K130IBacillus subtilis(E,E)-farnesyl diphosphate—0.0072—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant K130IBacillus subtilisisopentenyl diphosphate180.01341.3 × 10³ calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant L102SBacillus subtilis(E,E)-farnesyl diphosphate—0.0072—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant L102SBacillus subtilisisopentenyl diphosphate220.02031.1 × 10³ calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant L107SBacillus subtilis(E,E)-farnesyl diphosphate—0.0053—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant L107SBacillus subtilisisopentenyl diphosphate16.50.0175943 calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant L94SBacillus subtilis(E,E)-farnesyl diphosphate—0.0508—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant L94SBacillus subtilisisopentenyl diphosphate20.0194100 calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant N127ABacillus subtilis(E,E)-farnesyl diphosphate—0.0082—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant N127ABacillus subtilisisopentenyl diphosphate18.50.0194954 calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant S100GBacillus subtilis(E,E)-farnesyl diphosphate—0.0103—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant S100GBacillus subtilisisopentenyl diphosphate14.330.01421.01 × 10³ calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant T76VBacillus subtilis(E,E)-farnesyl diphosphate—0.0084—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant T76VBacillus subtilisisopentenyl diphosphate16.420.0183897 calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant V93GBacillus subtilis(E,E)-farnesyl diphosphate—0.0741—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant V93GBacillus subtilisisopentenyl diphosphate1.170.022652.0 calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant Y103SBacillus subtilis(E,E)-farnesyl diphosphate—0.0098—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant Y103SBacillus subtilisisopentenyl diphosphate17.370.0188924 calc.pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant Y104SBacillus subtilis(E,E)-farnesyl diphosphate—0.02—pH 8.5, 37 °COpen
heptaprenyl diphosphate synthase (HepPP synthase; component I + component II)variant Y104SBacillus subtilisisopentenyl diphosphate3.580.0167215 calc.pH 8.5, 37 °COpen