Values CatLog read from this paper
Effect of modified nucleotides on Escherichia coli tRNAGlu structure and on its aminoacylation by glutamyl-tRNA synthetase. Predominant and distinct roles of the mnm5 and s2 modifications of U34
PMID 10583410doi:10.1046/j.1432-1327.1999.00965.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 5 records (10 values) from this paper: glutamyl-tRNA synthetase (GluRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutamyl-tRNA synthetase (GluRS) | Escherichia coli | Escherichia coli tRNAGlu modivariant-1 (Ψ13, m2A37, mnm5s2U34, T54, Ψ55, extra Ψ (probably Ψ65)) | 6.5 | 6 × 10⁻⁵ | 1.1 × 10⁵ calc. | pH 7.2, 37 °C | Open record: glutamyl-tRNA synthetase (GluRS), Escherichia coli tRNAGlu modivariant-1 (Ψ13, m2A37, mnm5s2U34, T54, Ψ55, extra Ψ (probably Ψ65)) |
| glutamyl-tRNA synthetase (GluRS) | Escherichia coli | Escherichia coli tRNAGlu modivariant-2 (Ψ13, m2A37, mnm5s2U34, T54, Ψ55 (predominant form in wild-type E. coli)) | 8.5 | 1.3 × 10⁻⁴ | 6.5 × 10⁴ calc. | pH 7.2, 37 °C | Open record: glutamyl-tRNA synthetase (GluRS), Escherichia coli tRNAGlu modivariant-2 (Ψ13, m2A37, mnm5s2U34, T54, Ψ55 (predominant form in wild-type E. coli)) |
| glutamyl-tRNA synthetase (GluRS) | Escherichia coli | Escherichia coli tRNAGlu modivariant-3 (Ψ13, A37, U34, T54, Ψ55 (no anticodon-loop modification)) | 1.5 | 0.012 | 120 calc. | pH 7.2, 37 °C | Open record: glutamyl-tRNA synthetase (GluRS), Escherichia coli tRNAGlu modivariant-3 (Ψ13, A37, U34, T54, Ψ55 (no anticodon-loop modification)) |
| glutamyl-tRNA synthetase (GluRS) | Escherichia coli | Escherichia coli tRNAGlu modivariant-4 (U13, A37, s2U34, T54, Ψ55) | 6.2 | 7.2 × 10⁻⁵ | 8.6 × 10⁴ calc. | pH 7.2, 37 °C | Open record: glutamyl-tRNA synthetase (GluRS), Escherichia coli tRNAGlu modivariant-4 (U13, A37, s2U34, T54, Ψ55) |
| glutamyl-tRNA synthetase (GluRS) | Escherichia coli | Escherichia coli tRNAGlu modivariant-5 (Ψ13, m2A37, s2U34, T54, Ψ55) | 9.6 | 1.2 × 10⁻⁴ | 8.0 × 10⁴ calc. | pH 7.2, 37 °C | Open record: glutamyl-tRNA synthetase (GluRS), Escherichia coli tRNAGlu modivariant-5 (Ψ13, m2A37, s2U34, T54, Ψ55) |