Values CatLog read from this paper

Mutational analysis of Asp51 of Anabaena azollae glutamine synthetase. D51E mutation confers resistance to the active site inhibitors L-methionine-DL-sulfoximine and phosphinothricin

PMID 10583418doi:10.1046/j.1432-1327.1999.00992.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (25 values) from this paper: glutamine synthetase from Anabaena azollae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.1.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
glutamine synthetase	Anabaena azollae	adenosine 5'-triphosphateTable 2	—	0.21	—	pH 7.5, 30 °C	Open record: glutamine synthetase, adenosine 5'-triphosphate
glutamine synthetase	Anabaena azollae	adenosine 5'-triphosphatein the text	—	—	193	pH 7.5, 30 °C	Open record: glutamine synthetase, adenosine 5'-triphosphate
glutamine synthetase	Anabaena azollae	ammoniumTable 2	—	0.05	—	pH 7.5, 30 °C	Open record: glutamine synthetase, ammonium
glutamine synthetase	Anabaena azollae	ammoniumin the text	—	—	435	pH 7.5, 30 °C	Open record: glutamine synthetase, ammonium
glutamine synthetase	Anabaena azollae	L-glutamateTable 2	42	1.5	28 calc.	pH 7.5, 30 °C	Open record: glutamine synthetase, L-glutamate
glutamine synthetase	Anabaena azollae	L-glutamatein the text	—	—	27.8	pH 7.5, 30 °C	Open record: glutamine synthetase, L-glutamate
glutamine synthetasevariant D51A	Anabaena azollae	adenosine 5'-triphosphate	—	0.05	—	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51A, adenosine 5'-triphosphate
glutamine synthetasevariant D51A	Anabaena azollae	ammonium	—	12.45	—	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51A, ammonium
glutamine synthetasevariant D51A	Anabaena azollae	L-glutamate	2.6	0.32	8.1 calc.	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51A, L-glutamate
glutamine synthetasevariant D51E	Anabaena azollae	adenosine 5'-triphosphateTable 2	—	10.6	—	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51E, adenosine 5'-triphosphate
glutamine synthetasevariant D51E	Anabaena azollae	adenosine 5'-triphosphatein the text	—	—	0.03	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51E, adenosine 5'-triphosphate
glutamine synthetasevariant D51E	Anabaena azollae	ammonium	—	0.55	—	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51E, ammonium
glutamine synthetasevariant D51E	Anabaena azollae	L-glutamateTable 2	0.33	16	0.021 calc.	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51E, L-glutamate
glutamine synthetasevariant D51E	Anabaena azollae	L-glutamatein the text	—	—	0.02	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51E, L-glutamate
glutamine synthetasevariant D51N	Anabaena azollae	adenosine 5'-triphosphate	—	0.12	—	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51N, adenosine 5'-triphosphate
glutamine synthetasevariant D51N	Anabaena azollae	ammonium	—	0.92	—	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51N, ammonium
glutamine synthetasevariant D51N	Anabaena azollae	L-glutamate	0.96	0.85	1.1 calc.	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51N, L-glutamate
glutamine synthetasevariant D51S	Anabaena azollae	adenosine 5'-triphosphate	—	0.07	—	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51S, adenosine 5'-triphosphate
glutamine synthetasevariant D51S	Anabaena azollae	ammonium	—	5.95	—	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51S, ammonium
glutamine synthetasevariant D51S	Anabaena azollae	L-glutamate	13.1	0.37	35 calc.	pH 7.5, 30 °C	Open record: glutamine synthetase variant D51S, L-glutamate