Values CatLog read from this paper
Mapping of ATP binding regions in poly(A) polymerases by photoaffinity labeling and by mutational analysis identifies a domain conserved in many nucleotidyltransferases
PMID 10595540doi:10.1110/ps.8.11.2380Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 35 records (70 values) from this paper: poly(A) polymerase from Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 35 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| poly(A) polymerase | Bos taurus | adenosine 5'-triphosphate | 0.19 | 0.26 | 0.73 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase, adenosine 5'-triphosphate |
| poly(A) polymerase | Bos taurus | adenosine 5'-triphosphatebPAP-513: C-terminal deletion; recombinant from E. coli | 0.28 | 0.24 | 1.2 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase, adenosine 5'-triphosphate |
| poly(A) polymerasevariant D208A | Bos taurus | adenosine 5'-triphosphate | 0.31 | 0.42 | 0.74 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant D208A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant D218A | Bos taurus | adenosine 5'-triphosphate | 0.41 | 0.31 | 1.3 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant D218A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant E209A | Bos taurus | adenosine 5'-triphosphate | 0.44 | 0.33 | 1.3 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant E209A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant F100D | Bos taurus | adenosine 5'-triphosphate | 0.0043 | 0.51 | 0.0084 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant F100D, adenosine 5'-triphosphate |
| poly(A) polymerasevariant F220R | Bos taurus | adenosine 5'-triphosphate | 0.36 | 1.04 | 0.35 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant F220R, adenosine 5'-triphosphate |
| poly(A) polymerasevariant F220S | Bos taurus | adenosine 5'-triphosphate | 0.42 | 1.28 | 0.33 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant F220S, adenosine 5'-triphosphate |
| poly(A) polymerasevariant F220Y | Bos taurus | adenosine 5'-triphosphate | 0.62 | 0.31 | 2.0 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant F220Y, adenosine 5'-triphosphate |
| poly(A) polymerasevariant F243A | Bos taurus | adenosine 5'-triphosphate | 0.21 | 0.77 | 0.27 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant F243A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant G203H | Bos taurus | adenosine 5'-triphosphate | 7 × 10⁻⁴ | 0.29 | 0.0024 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant G203H, adenosine 5'-triphosphate |
| poly(A) polymerasevariant H212A | Bos taurus | adenosine 5'-triphosphate | 0.53 | 0.17 | 3.1 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant H212A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant I217T | Bos taurus | adenosine 5'-triphosphate | 0.35 | 0.25 | 1.4 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant I217T, adenosine 5'-triphosphate |
| poly(A) polymerasevariant K191A | Bos taurus | adenosine 5'-triphosphate | 0.81 | 0.3 | 2.7 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant K191A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant K228A | Bos taurus | adenosine 5'-triphosphate | 0.09 | 1.01 | 0.089 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant K228A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant L105A | Bos taurus | adenosine 5'-triphosphate | 1.01 | 1.04 | 0.971 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant L105A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant L193M | Bos taurus | adenosine 5'-triphosphate | 1.09 | 0.33 | 3.3 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant L193M, adenosine 5'-triphosphate |
| poly(A) polymerasevariant L211K | Bos taurus | adenosine 5'-triphosphate | 0.52 | 0.33 | 1.6 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant L211K, adenosine 5'-triphosphate |
| poly(A) polymerasevariant L213K | Bos taurus | adenosine 5'-triphosphate | 0.41 | 0.37 | 1.1 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant L213K, adenosine 5'-triphosphate |
| poly(A) polymerasevariant L229R | Bos taurus | adenosine 5'-triphosphate | 0.26 | 0.46 | 0.57 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant L229R, adenosine 5'-triphosphate |
| poly(A) polymerasevariant N216A | Bos taurus | adenosine 5'-triphosphate | 0.39 | 0.26 | 1.5 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant N216A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant N216S/D218A/F220S | Bos taurus | adenosine 5'-triphosphate | 0.15 | 3.61 | 0.042 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant N216S/D218A/F220S, adenosine 5'-triphosphate |
| poly(A) polymerasevariant N219A | Bos taurus | adenosine 5'-triphosphate | 0.39 | 0.3 | 1.3 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant N219A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant R104A | Bos taurus | adenosine 5'-triphosphate | 2.66 | 0.45 | 5.9 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant R104A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant R172G | Bos taurus | adenosine 5'-triphosphate | 0.71 | 0.3 | 2.4 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant R172G, adenosine 5'-triphosphate |
| poly(A) polymerasevariant R221A | Bos taurus | adenosine 5'-triphosphate | 0.42 | 0.37 | 1.1 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant R221A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant R225A | Bos taurus | adenosine 5'-triphosphate | 0.19 | 0.22 | 0.86 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant R225A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant R225N | Bos taurus | adenosine 5'-triphosphate | 0.11 | 0.15 | 0.73 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant R225N, adenosine 5'-triphosphate |
| poly(A) polymerasevariant R225Y | Bos taurus | adenosine 5'-triphosphate | 0.84 | 0.42 | 2.0 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant R225Y, adenosine 5'-triphosphate |
| poly(A) polymerasevariant S102A | Bos taurus | adenosine 5'-triphosphate | 0.37 | 0.33 | 1.1 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant S102A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant T177M | Bos taurus | adenosine 5'-triphosphate | 0.46 | 0.27 | 1.7 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant T177M, adenosine 5'-triphosphate |
| poly(A) polymerasevariant V214Q | Bos taurus | adenosine 5'-triphosphate | 0.27 | 0.38 | 0.71 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant V214Q, adenosine 5'-triphosphate |
| poly(A) polymerasevariant W230A | Bos taurus | adenosine 5'-triphosphate | 0.12 | 1.31 | 0.092 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant W230A, adenosine 5'-triphosphate |
| poly(A) polymerasevariant Y103G | Bos taurus | adenosine 5'-triphosphate | 0.73 | 0.72 | 1.0 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant Y103G, adenosine 5'-triphosphate |
| poly(A) polymerasevariant Y237A | Bos taurus | adenosine 5'-triphosphate | 0.16 | 0.88 | 0.18 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant Y237A, adenosine 5'-triphosphate |