Values CatLog read from this paper
Differences in substrate specificity and kinetic properties of the recombinant hexokinases HXK1 and HXK2 from Entamoeba histolytica
PMID 10613700doi:10.1016/s0166-6851(99)00164-4Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (30 values) from this paper: hexokinase HXK1, hexokinase HXK2 from Entamoeba histolytica.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| hexokinase HXK1 | Entamoeba histolytica | adenosine 5'-triphosphate | — | 0.183 | — | pH 8, 30 °C | Open record: hexokinase HXK1, adenosine 5'-triphosphate |
| hexokinase HXK1 | Entamoeba histolytica | D-glucoseoptimal preparation | 284 | 0.059 | 4.8 × 10³ calc. | pH 8, 30 °C | Open record: hexokinase HXK1, D-glucose |
| hexokinase HXK1 | Entamoeba histolytica | D-glucose2 mM ATP; triplicate | 200 | 0.067 | 3.0 × 10³ calc. | pH 8, 30 °C | Open record: hexokinase HXK1, D-glucose |
| hexokinase HXK1 | Entamoeba histolytica | D-mannose | — | 1.03 | — | pH 8, 30 °C | Open record: hexokinase HXK1, D-mannose |
| hexokinase HXK1 | Entamoeba histolytica | D-mannose | 180 | — | — | pH 8, 30 °C | Open record: hexokinase HXK1, D-mannose |
| hexokinase HXK1variant I270S (B2) | Entamoeba histolytica | D-glucose | 153 | 0.134 | 1.14 × 10³ calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant I270S (B2), D-glucose |
| hexokinase HXK1variant R265T/Y266F (C) | Entamoeba histolytica | D-glucose | 110 | 0.094 | 1.2 × 10³ calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant R265T/Y266F (C), D-glucose |
| hexokinase HXK1variant S144T (A) | Entamoeba histolytica | D-glucose | 165 | 0.047 | 3.5 × 10³ calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant S144T (A), D-glucose |
| hexokinase HXK1variant S144T/R265T/Y266F (A+C) | Entamoeba histolytica | D-glucose | 145 | 0.12 | 1.2 × 10³ calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant S144T/R265T/Y266F (A+C), D-glucose |
| hexokinase HXK1variant S144T/V183T/I270S (A+B1+B2) | Entamoeba histolytica | D-glucose | 102 | 1.06 | 96.2 calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant S144T/V183T/I270S (A+B1+B2), D-glucose |
| hexokinase HXK1variant S144T/V183T/R265T/Y266F/I270S (A+B1+B2+C) | Entamoeba histolytica | D-glucose | 40 | 1.4 | 29 calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant S144T/V183T/R265T/Y266F/I270S (A+B1+B2+C), D-glucose |
| hexokinase HXK1variant V183T (B1) | Entamoeba histolytica | D-glucose | 186 | 0.231 | 805 calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant V183T (B1), D-glucose |
| hexokinase HXK1variant V183T/I270S (B1+B2) | Entamoeba histolytica | D-glucose | 133 | 0.99 | 130 calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant V183T/I270S (B1+B2), D-glucose |
| hexokinase HXK1variant V183T/R265T/Y266F/I270S (B1+B2+C) | Entamoeba histolytica | D-glucose | 83 | 1.95 | 43 calc. | pH 8, 30 °C | Open record: hexokinase HXK1 variant V183T/R265T/Y266F/I270S (B1+B2+C), D-glucose |
| hexokinase HXK2 | Entamoeba histolytica | adenosine 5'-triphosphate | — | 0.654 | — | pH 8, 30 °C | Open record: hexokinase HXK2, adenosine 5'-triphosphate |
| hexokinase HXK2 | Entamoeba histolytica | D-glucoseoptimal preparation | 237 | 0.047 | 5.0 × 10³ calc. | pH 8, 30 °C | Open record: hexokinase HXK2, D-glucose |
| hexokinase HXK2 | Entamoeba histolytica | D-glucose2 mM ATP; triplicate | 189 | 0.033 | 5.7 × 10³ calc. | pH 8, 30 °C | Open record: hexokinase HXK2, D-glucose |