Values CatLog read from this paper
Threonine 201 in the diiron enzyme toluene 4-monooxygenase is not required for catalysis
PMID 10651645doi:10.1021/bi992187gOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (12 values) from this paper: toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complex from Pseudomonas mendocina.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complex | Pseudomonas mendocina | toluene | 1.3 | 0.005 | 300 | pH 7.5, 25 °C | Open record: toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complex, toluene |
| toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complexvariant T201A | Pseudomonas mendocina | toluene | 1 | 0.006 | 170 | pH 7.5, 25 °C | Open record: toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complex variant T201A, toluene |
| toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complexvariant T201G | Pseudomonas mendocina | toluene | 0.6 | 0.009 | 70 | pH 7.5, 25 °C | Open record: toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complex variant T201G, toluene |
| toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complexvariant T201S | Pseudomonas mendocina | toluene | 1.2 | 0.006 | 200 | pH 7.5, 25 °C | Open record: toluene 4-monooxygenase hydroxylase (T4MOH), assayed in the four-protein toluene 4-monooxygenase (T4MO) complex variant T201S, toluene |