Values CatLog read from this paper
Mutational analysis of 4-coumarate:CoA ligase identifies functionally important amino acids and verifies its close relationship to other adenylate-forming enzymes
PMID 10664468doi:10.1016/s0014-5793(00)01133-9Open in Browse
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CatLog read 13 records (13 values) from this paper: 4-coumarate:CoA ligase 2 (At4CL2) from Arabidopsis thaliana.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-coumarate:CoA ligase 2 (At4CL2) | Arabidopsis thaliana | adenosine 5'-triphosphate | — | 0.163 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2), adenosine 5'-triphosphate | |
| 4-coumarate:CoA ligase 2 (At4CL2) | Arabidopsis thaliana | adenosine 5'-triphosphateuntagged At4CL2 (lacking the N-terminal His6 tag) | — | 0.16 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2), adenosine 5'-triphosphate | |
| 4-coumarate:CoA ligase 2 (At4CL2) | Arabidopsis thaliana | caffeate | — | 0.024 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2), caffeate | |
| 4-coumarate:CoA ligase 2 (At4CL2) | Arabidopsis thaliana | caffeateuntagged At4CL2 (lacking the N-terminal His6 tag) | — | 0.025 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2), caffeate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant C403A | Arabidopsis thaliana | adenosine 5'-triphosphate | — | 0.173 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant C403A, adenosine 5'-triphosphate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant C403A | Arabidopsis thaliana | caffeate | — | 0.018 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant C403A, caffeate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant E401Q | Arabidopsis thaliana | adenosine 5'-triphosphate | — | 0.24 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant E401Q, adenosine 5'-triphosphate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant E401Q | Arabidopsis thaliana | caffeate | — | 0.028 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant E401Q, caffeate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant K211S | Arabidopsis thaliana | adenosine 5'-triphosphate | — | 0.151 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant K211S, adenosine 5'-triphosphate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant K211S | Arabidopsis thaliana | caffeate | — | 0.014 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant K211S, caffeate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant K457S | Arabidopsis thaliana | adenosine 5'-triphosphate | — | 0.029 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant K457S, adenosine 5'-triphosphate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant K457S | Arabidopsis thaliana | caffeate | — | 0.005 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant K457S, caffeate | |
| 4-coumarate:CoA ligase 2 (At4CL2)variant R449Q | Arabidopsis thaliana | adenosine 5'-triphosphate | — | 1.507 | — | Open record: 4-coumarate:CoA ligase 2 (At4CL2) variant R449Q, adenosine 5'-triphosphate |