Values CatLog read from this paper
The RadA protein from a hyperthermophilic archaeon Pyrobaculum islandicum is a DNA-dependent ATPase that exhibits two disparate catalytic modes, with a transition temperature at 75 degrees C
PMID 10672022doi:10.1046/j.1432-1327.2000.01108.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (30 values) from this paper: RadA protein (DNA-dependent ATPase) from Pyrobaculum islandicum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate1 µM RadA; various concentrations of ATP; no DNA cofactor | 0.018 | — | — | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate1 µM RadA; various concentrations of ATP; 10 mM MgCl2 | 0.059 | 0.065 | 0.91 | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate1 µM RadA; various concentrations of ATP; 10 mM MgCl2 | 0.067 | — | — | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate1 µM RadA; various concentrations of ATP; 10 mM MgCl2 | 0.04 | — | — | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate1 µM RadA; various concentrations of ATP; 10 mM MgCl2 | 0.052 | 0.054 | 0.96 | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate1 µM RadA; various concentrations of ATP; 10 mM MgCl2 | 0.013 | — | — | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate1 µM RadA; various concentrations of ATP; Table 1 | 0.187 | 2.7 | 0.07 | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate | 0.015 | — | — | pH 7.7, 37 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate | 0.025 | 0.409 | 0.062 | pH 7.7, 55 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate | 0.042 | 0.123 | 0.34 | pH 7.7, 65 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate | 0.048 | 0.096 | 0.5 | pH 7.7, 70 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate | 0.064 | — | — | pH 7.7, 80 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate | 0.065 | — | — | pH 7.7, 82 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate | 0.068 | — | — | pH 7.7, 85 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphate | 0.028 | — | — | pH 7.7, 90 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphateFig. 5A assay: 1 µM RadA; 10 µM poly(dT); 0.2 µCi [α-32P]ATP | 0.056 | — | — | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphateFig. 5A assay: 1 µM RadA; 10 µM poly(dT); 0.2 µCi [α-32P]ATP | 0.057 | — | — | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |
| RadA protein (DNA-dependent ATPase) | Pyrobaculum islandicum | adenosine 5'-triphosphateFig. 5A assay: 1 µM RadA; 10 µM poly(dT); 0.2 µCi [α-32P]ATP | 0.059 | — | — | pH 7.7, 75 °C | Open record: RadA protein (DNA-dependent ATPase), adenosine 5'-triphosphate |