Values CatLog read from this paper
Exploring substrate binding and discrimination in fructose1, 6-bisphosphate and tagatose 1,6-bisphosphate aldolases
PMID 10712619doi:10.1046/j.1432-1327.2000.01191.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 37 records (95 values) from this paper: fructose 1,6-bisphosphate aldolase (Class II), tagatose 1,6-bisphosphate aldolase (AgaY) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| fructose 1,6-bisphosphate aldolase (Class II) | Escherichia coli | D-fructose 1,6-bisphosphate | 1.8 | 0.021 | 86 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II), D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II) | Escherichia coli | D-fructose 1,6-bisphosphate | 10.5 | 0.17 | 62 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II), D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II) | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.015 | 0.35 | 0.043 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II), D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II) | Escherichia coli | dihydroxyacetone phosphate | 2.77 | 0.03 | 92 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II), dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D288A | Escherichia coli | D-fructose 1,6-bisphosphate | — | — | 2.17 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D288A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D288A | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.0068 | 0.36 | 0.018 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D288A, D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant F67H | Escherichia coli | D-fructose 1,6-bisphosphate | 13.3 | 0.21 | 63.5 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant F67H, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant F67H | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.0072 | 0.32 | 0.022 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant F67H, D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant G64T | Escherichia coli | D-fructose 1,6-bisphosphate | 2.67 | 0.2 | 13 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant G64T, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant G64T | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.0128 | 0.14 | 0.092 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant G64T, D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant K325A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.15 | 0.04 | 3.8 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant K325A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant K325A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.58 | 0.38 | 1.53 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant K325A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant K325A | Escherichia coli | dihydroxyacetone phosphate | 0.38 | 0.04 | 9.6 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant K325A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant N35A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.162 | 0.9 | 0.18 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant N35A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant N35A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.1 | 0.032 | 3.1 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant N35A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant N35A | Escherichia coli | dihydroxyacetone phosphate | 0.85 | 0.46 | 1.8 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant N35A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.75 | 0.043 | 17 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A | Escherichia coli | D-fructose 1,6-bisphosphate | 8.5 | 0.22 | 38.7 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.0102 | 0.38 | 0.027 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A, D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A | Escherichia coli | dihydroxyacetone phosphate | 1.97 | 0.21 | 9.4 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A/F67H | Escherichia coli | D-fructose 1,6-bisphosphate | 7.33 | 0.55 | 13 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A/F67H, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A/F67H | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.0132 | 0.16 | 0.082 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A/F67H, D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A/G64T/F67H | Escherichia coli | D-fructose 1,6-bisphosphate | 0.18 | 5.9 | 0.032 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A/G64T/F67H, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A/G64T/F67H | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.0063 | 0.19 | 0.033 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A/G64T/F67H, D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A/S61T/D288A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.048 | 1.7 | 0.028 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A/S61T/D288A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant Q59A/S61T/D288A | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.0092 | 0.23 | 0.04 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant Q59A/S61T/D288A, D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.85 | 2.7 | 0.31 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.37 | 1.4 | 0.26 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61A | Escherichia coli | dihydroxyacetone phosphate | 1.27 | 0.39 | 3.2 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61T | Escherichia coli | D-fructose 1,6-bisphosphate | 1.58 | 0.082 | 19 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61T, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61T | Escherichia coli | D-fructose 1,6-bisphosphate | 6.33 | 0.43 | 14.7 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61T, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61T | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.013 | 0.4 | 0.033 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61T, D-tagatose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61T | Escherichia coli | dihydroxyacetone phosphate | 2.02 | 0.055 | 37 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61T, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61T/G64T/F67H | Escherichia coli | D-fructose 1,6-bisphosphate | 0.22 | 0.2 | 1.08 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61T/G64T/F67H, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant S61T/G64T/F67H | Escherichia coli | D-tagatose 1,6-bisphosphate | 0.022 | 0.18 | 0.12 | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant S61T/G64T/F67H, D-tagatose 1,6-bisphosphate |
| tagatose 1,6-bisphosphate aldolase (AgaY) | Escherichia coli | D-fructose 1,6-bisphosphate | 0.068 | 1.3 | 0.053 | 30 °C | Open record: tagatose 1,6-bisphosphate aldolase (AgaY), D-fructose 1,6-bisphosphate |
| tagatose 1,6-bisphosphate aldolase (AgaY) | Escherichia coli | D-tagatose 1,6-bisphosphate | 4.7 | 0.26 | 18 | 30 °C | Open record: tagatose 1,6-bisphosphate aldolase (AgaY), D-tagatose 1,6-bisphosphate |