Values CatLog read from this paper

Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution

PMID 10736164doi:10.1021/bi991483qOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (84 values) from this paper: beta-glucosidase CelB from Pyrococcus furiosus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
beta-glucosidase CelB	Pyrococcus furiosus	cellobiose	16	23	0.69	pH 4.8, 20 °C	Open record: beta-glucosidase CelB, cellobiose
beta-glucosidase CelB	Pyrococcus furiosus	cellobiose	670	14	48	pH 4.8, 90 °C	Open record: beta-glucosidase CelB, cellobiose
beta-glucosidase CelB	Pyrococcus furiosus	lactose	13	52	0.25	pH 4.8, 20 °C	Open record: beta-glucosidase CelB, lactose
beta-glucosidase CelB	Pyrococcus furiosus	lactose	1.3 × 10³	120	11	pH 4.8, 90 °C	Open record: beta-glucosidase CelB, lactose
beta-glucosidase CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	9.1	0.7	13	pH 4.8, 20 °C	Open record: beta-glucosidase CelB, p-nitrophenyl-β-D-galactopyranoside
beta-glucosidase CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	2.4 × 10³	5	480	pH 4.8, 90 °C	Open record: beta-glucosidase CelB, p-nitrophenyl-β-D-galactopyranoside
beta-glucosidase CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	13	0.53	25	pH 4.8, 20 °C	Open record: beta-glucosidase CelB, p-nitrophenyl-β-D-glucopyranoside
beta-glucosidase CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	1.6 × 10³	0.42	3.9 × 10³	pH 4.8, 90 °C	Open record: beta-glucosidase CelB, p-nitrophenyl-β-D-glucopyranoside
beta-glucosidase CelBvariant A419T	Pyrococcus furiosus	cellobiose	19	25	0.75	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant A419T, cellobiose
beta-glucosidase CelBvariant A419T	Pyrococcus furiosus	lactose	15	83	0.18	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant A419T, lactose
beta-glucosidase CelBvariant A419T	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	14	4.5	3.1	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant A419T, p-nitrophenyl-β-D-galactopyranoside
beta-glucosidase CelBvariant A419T	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	40	1.3	31	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant A419T, p-nitrophenyl-β-D-glucopyranoside
beta-glucosidase CelBvariant M424V	Pyrococcus furiosus	cellobiose	18	16	1.1	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant M424V, cellobiose
beta-glucosidase CelBvariant M424V	Pyrococcus furiosus	lactose	21	97	0.22	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant M424V, lactose
beta-glucosidase CelBvariant M424V	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	12	3.4	3.4	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant M424V, p-nitrophenyl-β-D-galactopyranoside
beta-glucosidase CelBvariant M424V	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	40	0.92	43	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant M424V, p-nitrophenyl-β-D-glucopyranoside
beta-glucosidase CelBvariant N415S	Pyrococcus furiosus	cellobiose	20	20	1	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant N415S, cellobiose
beta-glucosidase CelBvariant N415S	Pyrococcus furiosus	cellobiose	240	38	6.4	pH 4.8, 90 °C	Open record: beta-glucosidase CelB variant N415S, cellobiose
beta-glucosidase CelBvariant N415S	Pyrococcus furiosus	lactose	21	95	0.22	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant N415S, lactose
beta-glucosidase CelBvariant N415S	Pyrococcus furiosus	lactose	530	220	2.4	pH 4.8, 90 °C	Open record: beta-glucosidase CelB variant N415S, lactose
beta-glucosidase CelBvariant N415S	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	4.2	1.2	3.5	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant N415S, p-nitrophenyl-β-D-galactopyranoside
beta-glucosidase CelBvariant N415S	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	1 × 10³	8.5	120	pH 4.8, 90 °C	Open record: beta-glucosidase CelB variant N415S, p-nitrophenyl-β-D-galactopyranoside
beta-glucosidase CelBvariant N415S	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	47	1.1	43	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant N415S, p-nitrophenyl-β-D-glucopyranoside
beta-glucosidase CelBvariant N415S	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	1.4 × 10³	1.4	1 × 10³	pH 4.8, 90 °C	Open record: beta-glucosidase CelB variant N415S, p-nitrophenyl-β-D-glucopyranoside
beta-glucosidase CelBvariant T371A	Pyrococcus furiosus	cellobiose	15	11	1.4	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant T371A, cellobiose
beta-glucosidase CelBvariant T371A	Pyrococcus furiosus	lactose	17	52	0.33	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant T371A, lactose
beta-glucosidase CelBvariant T371A	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	11	1.3	8.2	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant T371A, p-nitrophenyl-β-D-galactopyranoside
beta-glucosidase CelBvariant T371A	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	26	0.57	45	pH 4.8, 20 °C	Open record: beta-glucosidase CelB variant T371A, p-nitrophenyl-β-D-glucopyranoside