Values CatLog read from this paper
Histidine 295 and histidine 510 are crucial for the enzymatic degradation of heparan sulfate by heparinase III
PMID 10747789doi:10.1021/bi992514kOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (24 values) from this paper: heparinase III from Flavobacterium heparinum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| heparinase III | Flavobacterium heparinum | heparan sulfatephosphate buffer without imidazole (Table 2 footnote b) | 94 | 0.143 | 660 calc. | pH 7.6, 35 °C | Open record: heparinase III, heparan sulfate |
| heparinase III | Flavobacterium heparinum | heparan sulfaterecombinant; without its putative signal sequence | 78 | 0.08 | 980 calc. | pH 7.6, 35 °C | Open record: heparinase III, heparan sulfate |
| heparinase IIIvariant H110A | Flavobacterium heparinum | heparan sulfate | 37 | 0.009 | 4.1 × 10³ calc. | pH 7.6, 35 °C | Open record: heparinase III variant H110A, heparan sulfate |
| heparinase IIIvariant H139A | Flavobacterium heparinum | heparan sulfate | 68 | 0.191 | 360 calc. | pH 7.6, 35 °C | Open record: heparinase III variant H139A, heparan sulfate |
| heparinase IIIvariant H152A | Flavobacterium heparinum | heparan sulfate | 83 | 0.058 | 1.4 × 10³ calc. | pH 7.6, 35 °C | Open record: heparinase III variant H152A, heparan sulfate |
| heparinase IIIvariant H225A | Flavobacterium heparinum | heparan sulfate | 22 | 0.08 | 280 calc. | pH 7.6, 35 °C | Open record: heparinase III variant H225A, heparan sulfate |
| heparinase IIIvariant H234A | Flavobacterium heparinum | heparan sulfate | 23 | 0.075 | 310 calc. | pH 7.6, 35 °C | Open record: heparinase III variant H234A, heparan sulfate |
| heparinase IIIvariant H241A | Flavobacterium heparinum | heparan sulfate | 5 | 0.016 | 310 calc. | pH 7.6, 35 °C | Open record: heparinase III variant H241A, heparan sulfate |
| heparinase IIIvariant H36A | Flavobacterium heparinum | heparan sulfate | 86 | 0.098 | 880 calc. | pH 7.6, 35 °C | Open record: heparinase III variant H36A, heparan sulfate |
| heparinase IIIvariant H424A | Flavobacterium heparinum | heparan sulfate | 24 | 0.059 | 410 calc. | pH 7.6, 35 °C | Open record: heparinase III variant H424A, heparan sulfate |
| heparinase IIIvariant H469A | Flavobacterium heparinum | heparan sulfate | 100 | 0.071 | 1.4 × 10³ calc. | pH 7.6, 35 °C | Open record: heparinase III variant H469A, heparan sulfate |
| heparinase IIIvariant H539A | Flavobacterium heparinum | heparan sulfate | 132 | 0.092 | 1.4 × 10³ calc. | pH 7.6, 35 °C | Open record: heparinase III variant H539A, heparan sulfate |