Values CatLog read from this paper
The aglycone specificity-determining sites are different in 2, 4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA)-glucosidase (Maize beta -glucosidase) and dhurrinase (Sorghum beta -glucosidase)
PMID 10748038doi:10.1074/jbc.m001609200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 27 records (81 values) from this paper: Glu1/Dhr1 chimeric beta-glucosidase, beta-glucosidase isozyme Glu1 (DIMBOA-glucosidase), Dhr1/Glu1 chimeric beta-glucosidase and 1 more from maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region), Zea mays, sorghum (Dhr1 N-terminal region) and maize (Glu1 C-terminal region) and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-glucosidase isozyme Dhr1 (dhurrinase) | Sorghum bicolor | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 77.7 | 0.05 | 1494 | pH 5.8, 37 °C | Open record: beta-glucosidase isozyme Dhr1 (dhurrinase), p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| beta-glucosidase isozyme Glu1 (DIMBOA-glucosidase) | Zea mays | 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one | 29.16 | 0.098 | 297.6 | pH 5.8, 37 °C | Open record: beta-glucosidase isozyme Glu1 (DIMBOA-glucosidase), 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one |
| beta-glucosidase isozyme Glu1 (DIMBOA-glucosidase) | Zea mays | o-nitrophenyl-beta-D-glucoside | 19.83 | 1.3 | 15.25 | pH 5.8, 25 °C | Open record: beta-glucosidase isozyme Glu1 (DIMBOA-glucosidase), o-nitrophenyl-beta-D-glucoside |
| beta-glucosidase isozyme Glu1 (DIMBOA-glucosidase) | Zea mays | p-nitrophenyl-beta-D-glucoside | 24.16 | 0.38 | 63.57 | pH 5.8, 25 °C | Open record: beta-glucosidase isozyme Glu1 (DIMBOA-glucosidase), p-nitrophenyl-beta-D-glucoside |
| Dhr1/Glu1 chimeric beta-glucosidasevariant chim 17 | sorghum (Dhr1 N-terminal region) and maize (Glu1 C-terminal region) | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 18.68 | 0.15 | 124.3 | pH 5.8, 37 °C | Open record: Dhr1/Glu1 chimeric beta-glucosidase variant chim 17, p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| Dhr1/Glu1 chimeric beta-glucosidasevariant chim 23 | sorghum (Dhr1 N-terminal region) and maize (Glu1 C-terminal region) | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 53.96 | 0.04 | 1350 | pH 5.8, 37 °C | Open record: Dhr1/Glu1 chimeric beta-glucosidase variant chim 23, p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| Dhr1/Glu1 chimeric beta-glucosidasevariant chim 39 | sorghum (Dhr1 N-terminal region) and maize (Glu1 C-terminal region) | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 23.41 | 0.12 | 195 | pH 5.8, 37 °C | Open record: Dhr1/Glu1 chimeric beta-glucosidase variant chim 39, p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 15 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one | 24.02 | 0.106 | 226.6 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 15, 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 15 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | o-nitrophenyl-beta-D-glucoside | 31.19 | 1.39 | 22.43 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 15, o-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 15 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 13.46 | 0.51 | 26.1 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 15, p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 15 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-nitrophenyl-beta-D-glucoside | 34.26 | 0.35 | 97.88 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 15, p-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 16 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one | 19.54 | 0.2 | 97.7 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 16, 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 16 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | o-nitrophenyl-beta-D-glucoside | 54.02 | 1.23 | 43.91 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 16, o-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 16 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 31.83 | 0.1 | 303.1 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 16, p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 16 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-nitrophenyl-beta-D-glucoside | 90.8 | 0.42 | 216.4 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 16, p-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 2 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one | 43.3 | 0.205 | 211.4 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 2, 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 2 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | o-nitrophenyl-beta-D-glucoside | 64.25 | 1.36 | 47.24 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 2, o-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 2 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 22.7 | 0.36 | 62.4 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 2, p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 2 | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-nitrophenyl-beta-D-glucoside | 107.9 | 0.39 | 276.7 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 2, p-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 21 (F466S/A467S/F469Y/Y473F) | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one | 20.15 | 0.104 | 195.2 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 21 (F466S/A467S/F469Y/Y473F), 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 21 (F466S/A467S/F469Y/Y473F) | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | o-nitrophenyl-beta-D-glucoside | 37.05 | 1.4 | 26.46 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 21 (F466S/A467S/F469Y/Y473F), o-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 21 (F466S/A467S/F469Y/Y473F) | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 46.02 | 0.09 | 494.8 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 21 (F466S/A467S/F469Y/Y473F), p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 21 (F466S/A467S/F469Y/Y473F) | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-nitrophenyl-beta-D-glucoside | 87.24 | 0.36 | 242.6 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 21 (F466S/A467S/F469Y/Y473F), p-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 22 (N481E/N483G/T485E/Y487T/E490R) | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one | 25.67 | 0.098 | 261.9 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 22 (N481E/N483G/T485E/Y487T/E490R), 2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 22 (N481E/N483G/T485E/Y487T/E490R) | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | o-nitrophenyl-beta-D-glucoside | 45.65 | 1.02 | 44.75 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 22 (N481E/N483G/T485E/Y487T/E490R), o-nitrophenyl-beta-D-glucoside |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 22 (N481E/N483G/T485E/Y487T/E490R) | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-hydroxy-(S)-mandelonitrile-beta-D-glucose | 27.17 | 0.18 | 150.9 | pH 5.8, 37 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 22 (N481E/N483G/T485E/Y487T/E490R), p-hydroxy-(S)-mandelonitrile-beta-D-glucose |
| Glu1/Dhr1 chimeric beta-glucosidasevariant chim 22 (N481E/N483G/T485E/Y487T/E490R) | maize (Glu1 N-terminal region) and sorghum (Dhr1 C-terminal region) | p-nitrophenyl-beta-D-glucoside | 68.9 | 0.35 | 196.4 | pH 5.8, 25 °C | Open record: Glu1/Dhr1 chimeric beta-glucosidase variant chim 22 (N481E/N483G/T485E/Y487T/E490R), p-nitrophenyl-beta-D-glucoside |